Mammalian genomes
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The heart is one of the least regenerative organs in the human body; adult cardiac myocytes divide at extremely low frequency. Therefore, meaningful induction of cardiac regeneration requires in-depth understanding of myocyte cell-cycle control. Recent insights into how myocytes can be coaxed into duplicating in vivo might inform emerging therapeutics.
3p vibransone 28-03-2024 2 2 Download
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Circadian clocks are endogenous oscillators that control 24-h physiological and behavioral processes. The central circadian clock exerts control over myriad aspects of mammalian physiology, including the regulation of sleep, metabolism, and the immune system.
16p vibransone 28-03-2024 2 1 Download
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Single-cell genomics is a rapidly advancing field; however, most techniques are designed for mammalian cells. We present a single-cell sequencing pipeline for an intracellular parasite, Plasmodium falciparum, with a small genome of extreme base content.
23p vibransone 28-03-2024 1 1 Download
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Ebook "Genomics and systems biology of mammalian cell culture (Advances in biochemical engineering/biotechnology, Volume 127)" have gathered nine chapters written by authors from both academia and industry which present recent development and different facets of genomic and systems-biological approaches applied to mammalian cell cultures. The book will give some impulse and inspiration to the genomics and systems biology of mammalian cell cultures.
305p manmanthanhla0201 26-02-2024 1 1 Download
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Ebook "Genetic engineering: Principles and methods (Volume 28)" presents state-of-the-art discussions in modern genetics and genetic engineering. This focus affirms a commitment to publish important reviews of the broadest interest to geneticists and their colleagues in affiliated disciplines. Recent volumes have covered gene therapy research, genetic mapping, plant science and technology, transport protein biochemistry, and viral vectors in gene therapy, among other topics.
206p manmanthanhla0201 27-02-2024 7 1 Download
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Ebook "Zygotic genome activation: Methods and protocols" provides an overview of zygotic genome activation (ZGA) and the use of recent tools to elucidate the events that occur during ZGA. The chapters in this book cover topics such as: clearance of maternal RNAs, detection of miRNA in mammalian oocytes and embryos, RNA FISH to study ZGA in early mouse embryos, and CRISPR/Cas9-mediated gene targeting during embryogenesis in the mouse and pig.
271p lucchinguyen 28-12-2023 6 2 Download
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Neuregulin 3 (NRG3) plays a key role in central nervous system development and is a strong candidate for human mental disorders. Thus, genetic variation in NRG3 may have some impact on a variety of phenotypes in non-mammalian vertebrates. Recently, genome-wide screening for short insertions and deletions in chicken (Gallus gallus) genomes has provided useful information about structural variation in functionally important genes.
10p vinarcissa 21-03-2023 7 1 Download
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Formation of the vertebral column is a critical developmental stage in mammals. The strict control of this process has resulted in little variation in number of vertebrae across mammalian species and no variation within most mammalian species.
9p vinarcissa 21-03-2023 6 1 Download
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Genome-wide association studies (GWAS) have been successfully implemented in cattle research and breeding. However, moving from the associations to identify the causal variants and reveal underlying mechanisms have proven complicated.
12p vihagrid 30-01-2023 6 3 Download
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(BQ) Ebook Chemical and functional genomic approaches to stem cell biology and regenerative medicine: Part 1 presents the following content: Embryonic stem cells; adult stem cells; genomewide expression analysis technologies; genomic cdna and rnai functional profiling and its potential application to the study of mammalian stem cells; chemical technologies: probing biology with small molecules.
162p runordie7 30-08-2022 3 2 Download
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Alternative polyadenylation (APA) affects most mammalian genes. The genome-wide investigation of APA has been hampered by an inability to reliably profile it using conventional RNA-seq. We describe ‘Quantification of APA’ (QAPA), a method that infers APA from conventional RNA-seq data.
18p vimichaelfaraday 25-03-2022 15 1 Download
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Organogenesis is crucial for proper organ formation during mammalian embryonic development. However, the similarities and shared features between different organs and the cellular heterogeneity during this process at single-cell resolution remain elusive.
20p vimichaelfaraday 25-03-2022 16 1 Download
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N6 -methyladenosine (m6 A) is an important epitranscriptomic mark with high abundance in the brain. Recently, it has been found to be involved in the regulation of memory formation and mammalian cortical neurogenesis.
18p vimichaelfaraday 25-03-2022 13 1 Download
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Histone lysine acylations by short-chain fatty acids are distinct from the widely studied histone lysine acetylation in chromatin, although both modifications are regulated by primary metabolism in mammalian cells. It remains unknown whether and how histone acylation and acetylation interact to regulate gene expression in plants that have distinct regulatory pathways of primary metabolism.
14p vigalileogalilei 27-02-2022 8 1 Download
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Some exciting biological questions require quantifying thousands of proteins in single cells. To achieve this goal, we develop Single Cell ProtEomics by Mass Spectrometry (SCoPE-MS) and validate its ability to identify distinct human cancer cell types based on their proteomes. We use SCoPE-MS to quantify over a thousand proteins in differentiating mouse embryonic stem cells.
12p vigalileogalilei 27-02-2022 10 1 Download
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Co-transcriptional R-loops are abundant non-B DNA structures in mammalian genomes. DNA Topoisomerase I (Top1) is often thought to regulate R-loop formation owing to its ability to resolve both positive and negative supercoils. How Top1 regulates R-loop structures at a global level is unknown.
18p vigalileogalilei 27-02-2022 12 1 Download
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CTCF binding to DNA helps partition the mammalian genome into discrete structural and regulatory domains. Complete removal of CTCF from mammalian cells causes catastrophic genome dysregulation, likely due to widespread collapse of 3D chromatin looping and alterations to inter- and intra-TAD interactions within the nucleus.
17p vigalileogalilei 27-02-2022 13 1 Download
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DNA methylation is a heritable epigenetic mark, enabling stable but reversible gene repression. In mammalian cells, DNA methyltransferases (DNMTs) are responsible for modifying cytosine to 5-methylcytosine (5mC), which can be further oxidized by the TET dioxygenases to ultimately cause DNA demethylation.
15p vigalileogalilei 27-02-2022 17 1 Download
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CRISPR-Cas12a/Cpf1, a single RNA-guided endonuclease system, provides a promising tool for genome engineering. However, only three Cas12a orthologs have been employed for mammalian genome editing, and the editing efficiency as well as targeting coverage still requires improvements.
6p vigalileogalilei 27-02-2022 15 1 Download
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Transcription factor (TF) binding to regulatory DNA sites is a key determinant of cell identity within multi-cellular organisms and has been studied extensively in relation to site affinity and chromatin modifications. There has been a strong focus on the inference of TF-gene regulatory networks and TF-TF physical interaction networks.
23p vigalileogalilei 27-02-2022 15 1 Download