Marker based screening of F1

The present study was undertaken to confirm the hybridity of Brassica juncea L. F1 plants based on the amplification pattern of SSR markers. The complementary banding pattern of the male and female parents helped to confirm the genuineness of F1 plants developed. The seeds of two genotypes of Brassica juncea, RSPR-01 used as female parent and DonskajaIV used as male parent were raised in pots under field conditions and crossed to develop F1 seeds. Out of 20 random SSR primers used for the screening of parental genotypes for polymorphism, 5 primers were found polymorphic.

7p trinhthamhodang1213 30-05-2020 7 0   Download

Scab caused by Venturia inaequalis (Cke.) Wint. is the most important fungal disease of apple. The development and deployment of genetic resistance is reliable and a safe option to combat the dreaded disease with palpable advantage as compared to chemical control. A variety Firdous developed by SKUAST-K has been known for its resistance, however, genetic basis of resistance has not been proved or established till date.

6p nguaconbaynhay4 22-03-2020 11 0   Download

Cytoplasmic-genic male sterile based F1 hybrid is a revolutionary approach to break yield stagnation in pigeonpea. Hybrid vigor is positively correlated to genetic distance and allelic divergence of the parents. This study estimated genetic divergence of 8 ‘A’ lines, 2 ‘B’ lines and 19 ‘R’ lines of A2 and A4 cytoplasmic sources, and predicted best parental combinations using molecular markers. Among 390 EST-SSR markers screened, only 60 markers found polymorphic indicating very low degree of polymorphism (15.4%). Average allelic count was 2.26 with a range of 2-4 per loci. Mean PIC was 0.

12p cothumenhmong1 10-12-2019 14 1   Download