Mature embryo
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To evaluate the correlation between the concentration of AMH (Anti-mullerian hormone) with the number of oocytes and embryos obtained in general and in each maternal age group, in particular on infertile patients undergoing in vitro fertilization (IVF) combined with preimplantation genetic testing for aneuploidy (PGT-A).
9p videadpool 06-05-2023 3 2 Download
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Growing oocytes derived from small antral follicles (2-3 mm) have poor maturation rates and developmental competence compared to fully grown oocytes collected from large antral follicles (4-6 mm). Hence, a pre-in vitro maturation (Pre-IVM) subculture with in vitro maturation (IVM) was applied to support small oocytes growth.
8p vimalfoy 08-02-2023 3 1 Download
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This study examined the effects of Scriptaid, a histone deacetylase inhibitor (HDAC) during the first mitotic cell cycle on preimplantation development of mouse embryos generated by injection of round spermatid into mature oocytes.
8p vimalfoy 08-02-2023 4 1 Download
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GATA1 is a key transcription factor in the GATA family, and promotes the differentiation and maturation of red blood cell, which is essential for normal hematopoiesis. Our study provides cues for further studying the roles of GATA1 gene in fish development, and suggested a potential molecular mechanism by which NP leads to abnormal development of fish embryos.
13p vihagrid 30-01-2023 7 3 Download
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RNA-directed DNA methylation (RdDM) initiates cytosine methylation in all contexts and maintains asymmetric CHH methylation. Mature plant embryos show one of the highest levels of CHH methylation, and it has been suggested that RdDM is responsible for this hypermethylation.
14p viarchimedes 26-01-2022 13 1 Download
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The present paper was conducted to study the desiccation effect of poly ethylene glycol (PEG) in relation to embryo maturation, and germination. Abnormal malformed embryo explants were cultured on germination medium supplemented with different concentrations of PEG (15, 20 and 25 g l1 ) for two periods (15, 30 days) as desiccation treatments.
12p schindler 30-12-2021 9 1 Download
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The highest significant values of total soluble sugars and their fractions were noted in explant stage. Embryogenic callus stage induced a significant increase in total soluble sugars, free amino acids, phenols and indoles concentrations as compared to callus and mature somatic embryo.
8p schindler 30-12-2021 13 0 Download
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This callus was initiated from different zones: the basal part of the flower, the surface of the perianth segments, carpels and the receptacle. Meanwhile, direct shoot and somatic embryos were only obtained by cultured explants on different concentrations of ABA which contained the highest concentration of total soluble sugars and their fractions.
9p schindler 30-12-2021 7 0 Download
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The collapsed outer layer of the pericarp in mature fruit could reshaped and regain turgor pressure after soaking in water, this may help for rapid germination. Although quinoa has thin seed coat layers however it have thick cell walls could maintain the moisture of embryo and perisperm and increase seed hardness as response to the arid habitats.
9p schindler 30-12-2021 5 0 Download
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Phalaenopsis is one of the most remarkable commercial genera in Orchidaceae family. Using immature explants for tissue culture of orchid results in undesired characteristics such as genetic variation and long-lasting juvenility. In this study, we optimized a protocol for somatic embryogenesis of Phalaenopsis amabilis cv. Jinan, by using mature thin cell layer (TCL) explants to minimize immature explant limitations. We examined somatic embryogenesis from two types of mature explants, viz.
9p hanthienngao 30-11-2021 9 1 Download
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To establish an efficient in vitro regeneration protocol for orchardgrass (Dactylis glomerata L.), type of medium, several plant growth regulators and their various concentrations were evaluated in a factorial experiment for callus induction and plant regeneration. Endosperm supported mature embryo were used as explants and optimum response in callus induction was observed on Chu medium with 12 mg/L dicamba (3.6-dichloro-2-methoxybenzoic acid). The highest responding embryogenic callus (15%) was found with Murashige and Skoog medium and 8 mg/L 2.4-D (2,4-dichlorophenoxy acetic acid).
11p hanthienngao 30-11-2021 9 1 Download
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The results showed that penetration and normal fertilization rates in both TCM-199 groups are both higher than that of NCSU-37 group. Moreover, the cleavage and blastocyst rates, and cell numbers of blastocysts which is a criterion for embryo quality were all higher in TCM-199 groups, especially in the group supplemented with pFF. It might be concluded that TCM-199 media supplemented with either pFF or FBS are suitable for effective in vitro maturation of Landrace porcine oocytes collected in Vietnam.
7p spiritedaway36 25-11-2021 7 0 Download
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The liver is mainly hematopoietic in the embryo, and converts into a major metabolic organ in the adult. Therefore, it is intensively remodeled after birth to adapt and perform adult functions. Long non-coding RNAs (lncRNAs) are involved in organ development and cell differentiation, likely they have potential roles in regulating postnatal liver development.
13p vibeauty 23-10-2021 14 1 Download
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Research is conducted on the Ban Pig model with goals: To determine the potential of Ban Pig's oocyte exploitation; to determine cultivate conditions of the oocyte mature in Ban Pig; success in establishing create Pig’s embryo system through in vitro fertilization (IVF) and cloning (C).
28p beloveinhouse01 15-08-2021 11 3 Download
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Transcription is arrested in the late stage oocyte and therefore the maternal transcriptome stored in the oocyte provides nearly all the mRNA required for oocyte maturation, fertilization, and early cleavage of the embryo.
18p vilichoo2711 25-06-2021 17 1 Download
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Somatic embryogenesis in grapevines is a complex process that depends on many physiological and genetic factors. One of its main limitations is the process of precocious germination of the somatic embryos in differentiation medium.
16p vijichea2711 28-05-2021 11 1 Download
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Rice (Oryza sativa L.) is the foremost important food crop in the world especially in Asiatic Continent. It is ranking second to the wheat among the most cultivated cereals in the world. Rice has widest germplasm collection than other cereal crops. It has to be made productive through several achievements in rice breeding programme, especially in sustainable food grain production with quality concern and it is undoubtedly the most important cereal of the world providing 21 per cent of global human per capita energy and 15 per cent of per capita protein.
7p angicungduoc6 22-07-2020 14 2 Download
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The study was conducted to standardize an efficient protocol for callus induction, subsequent growth and in-vitro regeneration in six genotypes of proso millet viz., Gavhe, Sakhroli, Kalsuli, Vari No.10, Asond and Sukdhar. The seeds were surface sterilized with 70% ethanol for 1 minute + 1% HgCl2 for 10 minutes recorded maximum aseptic culture establishment in all genotypes. The mature embryo (seeds) of proso millet was cultured on MS medium supplemented with varying concentrations of 2,4-D (0.1-2.0 mg/l) and NAA (1.0-4.
11p angicungduoc6 20-07-2020 13 1 Download
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Coconut (Cocos nucifera L.), is an important perennial crop cultivated across the globe. The demand for quality planting materials is increasing across the world for the establishment of new orchards, rejuvenation of existing orchards and production of quality nuts. Plant tissue culture is an alternate approach for the production of quality planting material. In this investigation, influence of auxin and cytokinin on somatic embryogenesis in coconut is studied using sliced mature embryos as explants in modified Y3 media supplemented with various concentrations of 2,4-D and kinetin.
8p gaocaolon5 14-06-2020 15 2 Download
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Five maize lines prevalently used by breeders in the maize program were screened for their ability to form type II callus. Mature seeds were used as explant for in vitro culturing. One inbred line showed high embryogenic response and formed friable, type II calli and also exhibited successful plant regeneration. Ten culture media compositions based on Murashige and Skoog (1962) salts thus investigated differed with respect to the combinations of concentrations of auxins (IBA, NAA, picloram, 2, 4-D) and cytokinins (BAP, kinetin) were used.
9p angicungduoc5 14-06-2020 4 0 Download