Medication selection

The core list presents a list of minimum medicine needs for a basic health‐care system, listing the most efficacious, safe and cost‐effective medicines for priority conditions. Priority conditions are selected on the basis of current and estimated future public health relevance, and potential for safe and cost‐effective treatment. The complementary list presents essential medicines for priority diseases, for which specialized diagnostic or monitoring facilities, and/or specialist medical care, and/or specialist training are needed.

45p kikyou751998 23-05-2012 448 38   Download

Presentations: JNC-8 Blood Pressure and ACC/AHA Cholesterol Guideline Updates, Review key recommendations from recently published guidelines on blood pressure and cholesterol management. Discuss implications for PPRNet clinical quality measures. Invite you to consult. Hope content useful document serves the academic needs and research.

28p thucnn94 20-03-2016 53 7   Download

Dendrimeric peptides selective for microbial surfaces have been developed to achieve broad antimicrobial activity and low hemolytic activity to human erythrocytes. The dendrimeric core is an asymmetric lysine branching tethered with two to eight copies of a tetrapeptide (R4) or an octapeptide (R8). The R4 tetrapeptide (RLYR) contains a putative microbial surface recognition BHHB motif (B ¼ basic, H ¼ hydrophobic amino acid) found in protegrins and tachyplesins whereas the octapeptide R8 (RLYRKVYG) consists of an R4 and a degenerated R4 repeat....

10p research12 01-06-2013 41 6   Download

Here we show, for the ®rst time, thein vitro formation of ®lamentous aggregates of phosphorylated tau protein in SH-SY5Y human neuroblastoma cells. The formation of suchaberrant aggregates, similar to thoseoccurringinvivoin Alzheimer's disease and other tauopathies, requires okadaic acid, a phosphatase inhibitor, to increase the level of phos-phorylated tau, and hydroxynonenal, a product of oxidative stress that selectively adducts and modi®es phosphorylated tau.

6p research12 29-04-2013 34 2   Download

There is much evidence to indicate that cholesterol forms lateral membranemicrodomains (rafts), and to suggest their important role in cellular signaling. However, no probe has been produced to analyze cholesterol behavior, especially cholesterol movement in rafts, in real time. To obtain a potent tool for analyzing cholesterol dynamics in rafts, we prepared and characterized several truncated fragments of h-toxin (perfringolysin O), a cholesterol-binding cytolysin, whose chemicallymodified formhas been recently shown to bind selectively to rafts. ...

9p tumor12 22-04-2013 41 5   Download

Site-directedmutagenesis was used to investigate the control of 2-oxoacid cosubstrate selectivity by deacetoxycephalo-sporin C synthase. The wild-type enzyme has a requirement for 2-oxoglutarate and cannot efficiently use hydrophobic 2-oxoacids (e.g. 2-oxohexanoic acid, 2-oxo-4-methyl-penta-noic acid) as the cosubstrate. The followingmutant enzymes were produced: R258A, R258L, R258F, R258H and R258K. All of the mutants have broadened cosubstrate selectivity and were able to utilize hydrophobic 2-oxoacids.

7p tumor12 20-04-2013 44 3   Download

As human choriocarcinoma cells display many of the biochemical and morphological characteristics reported for in utero invasive trophoblast cells we have studied cho-lesterol supply from high density lipoproteins (HDL) to these cells.Binding properties of 125 I-labeled HDL sub-class 3 (HDL3)at4
C were similar for BeWo, JAr, and Jeg3 choriocarcinoma cell lines while degradation rates at 37
C were highest for BeWo.

12p tumor12 20-04-2013 35 2   Download

A library of random peptide sequences was used to select peptides that inhibit an anti-idiotypic catalytic Ig, immuno-globulin (IgG) 9G4H9, withab-lactamase-like activity. This library displays cyclic heptapeptides on the surface of bac-teriophagesand representsacollectionofupto4.5·10 9 peptides. The first selection step aimed at enriching the lib-rary in species that bind to the whole Ig molecule. The sec-ond stepwas todiscriminate peptides that bind topart of the molecule other than the active site. Selected peptides were then screened by surface plasmon resonance analysis.

7p fptmusic 16-04-2013 43 3   Download

In aqueous media, muscle pyruvate kinase is highly selective for K + over Na + . We now studied the selec-tivity of pyruvate kinase in water/dimethylsulfoxide mix-tures by measuring the activation and inhibition constants of K + and Na + , i.e. their binding to the monovalent and divalent cation binding sites of pyruvate kinase, respect-ively [Melchoir J.B. (1965) Biochemistry4, 1518–1525]. In 40% dimethylsulfoxide theK0.5 appfor K + and Na + were 190 and 64-fold lower than in water.Kiappfor K + and Na +decreased 116 and 135-fold between 20 and 40% dimethylsulfoxide. The ratios of Kiapp/K0.

9p fptmusic 16-04-2013 34 2   Download

The dimeric structure of seminal ribonuclease (BS-RNase) is maintained by noncovalent interactions and by two intersubunit disulfide bridges.Another unusual feature of this enzyme is its antitumour action, consisting in a cyto-toxic activity selective for malignant cells.This cytotoxic action is exerted when the protein reaches the cytosol of the affected cells, where it degrades ribosomal RNA, thus blocking protein synthesis and leading cells to death.

8p fptmusic 16-04-2013 43 2   Download

To investigate the structural function of the C-terminal amide group of endomorphin-2 (EM2, H-Tyr-Pro-Phe-Phe-NH2), an endogenous l-opioid receptor ligand, the solution conformations of EM2 and its C-terminal free acid (EM2OH, H-Tyr-Pro-Phe-Phe-OH) in TFE (trifluoroethanol), water (pH 2.7 and 5.2), and aqueous DPC (dodecylphosphocholine) micelles (pH 3.5 and 5.2) were investigated by the combination of 2D 1 H-NMR meas-urement and molecular modelling calculation.

19p fptmusic 12-04-2013 33 4   Download

The Hsp90 molecular chaperone catalyses the final activa-tion step of many of themost important regulatory proteins of eukaryotic cells. The antibiotics geldanamycin and rad-icicol act as highly selective inhibitors of in vivo Hsp90 function through their ability to bindwithin the ADP/ATP binding pocket of the chaperone. Drugs based on these compounds are now being developed as anticancer agents, their administration having the potential to inactivate sim-ultaneously several of the targets critical for counteracting multistep carcinogenesis. ...

7p fptmusic 12-04-2013 48 3   Download

Heteronuclear NMR relaxation measurements and hydro-gen exchange data have been used to characterize protein dynamics in the presence or absence of stabilizing solutes from hyperthermophiles. Rubredoxin from Desulfovibrio gigaswas selected as a model protein and the effect of diglycerol phosphate on its dynamic behaviour was studied. The presence of 100 mMdiglycerol phosphate induces a fourfold increase in the half-life for thermal denaturation ofD.

9p fptmusic 12-04-2013 39 3   Download

The tumor suppressor protein, p53, selectively binds to supercoiled (sc) DNA lacking the specific p53 consensus binding sequence (p53CON). Using p53 deletion mutants, we have previously shown that the p53 C-terminal DNA-binding site (CTDBS) is critical for this binding.

12p awards 05-04-2013 50 5   Download

We have used the circular dichroismand infrared spectra of a specially designed 50 protein database [Oberg, K.A., Ruysschaert, J.M. & Goormaghtigh, E. (2003)Protein Sci. 12, 2015–2031] in order to optimize the accuracy of spectroscopic protein secondary structure determination using multivariate statisticalanalysis methods. The results demonstrate that when the proteins are carefully selected for the diversity in their structure, no smaller subset of the database contains the necessary information to describe the entire set....

12p awards 05-04-2013 41 4   Download

Cellular functions are ultimately linked to metabolic fluxes brought about by thousands of chemical reactions and transport processes. The synthesisof theunderlying enzymes and membrane transporters causes the cell a certain
effort of energyandexternal resources.Considering that those cells should have had a selection advantage during natural evo-lution that enabled them to fulfil vital functions (such as growth, defence against toxic compounds, repair of DNA alterations, etc.)

18p dell39 03-04-2013 34 5   Download

Three protein factors IF1, IF2 and IF3 are involved in the initiation of translation in prokaryotes. No clear function has been assigned to the smallest of these three factors, IF1. Therefore, to investigate the role of this protein in the initi-ation process inEscherichia coliwe have mutated the cor-responding gene infA. Because IF1 is essential for cell viability and no mutant selection has so far been described, the infA gene in a plasmid was mutated by site-directed mutagenesis in a strain with a chromosomalinfA + gene, followedbydeletionof thisinfA + gene.

11p dell39 03-04-2013 45 5   Download

For 30 years, the field of medical device technology has struggled to identify the specifications for optimal biocompatibility. For the vast majority of devices, clinical experience has eventually confirmed that the best performance is achieved when biocom-patibility is equated with biological safety that is materials are selected on the basis of maximizing bioinertness and minimizing toxicity and irritation.

7p inspiron33 26-03-2013 57 5   Download

In two cycles of an error-prone PCR process, variants of formate dehy-drogenase from Candida boidiniiwere created which revealed an up to 4.4-fold (440%) higher residual activity after entrapment in polyacrylamide gels than the wild-type enzyme. These were identified in an assay using sin-gle precursor molecules of polyacrylamide instead of the complete gel for selection.

8p inspiron33 25-03-2013 48 5   Download

Papain-like cysteine proteases of pathogenic protozoa play important roles in parasite growth, differentiation and host cell invasion. The main cysteine proteases of Trypanosoma cruzi(cruzipain) and of Trypanosoma brucei (brucipain) are validated targets for the development of new chemothera-pies.

11p galaxyss3 21-03-2013 58 4   Download