Báo cáo khoa học: Directed evolution of formate dehydrogenase from Candida boidinii for improved stability during entrapment in polyacrylamide
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In two cycles of an error-prone PCR process, variants of formate dehy-drogenase from Candida boidiniiwere created which revealed an up to 4.4-fold (440%) higher residual activity after entrapment in polyacrylamide gels than the wild-type enzyme. These were identified in an assay using sin-gle precursor molecules of polyacrylamide instead of the complete gel for selection.
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