
Báo cáo khoa học: Directed evolution of trimethoprim resistance inEscherichia coli
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Directed evolution is a useful tool in the study of enzymes. It is used in this study to investigate the means by which resistance to the antibiotic trimeth-oprim develops in dihyrofolate reductase fromEscherichia coli. Mutants with clinical levels of resistance were obtained after only three generations.
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