Meristem cultures
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Lasora (Cordia myxa Roxb.) is commonly grown as minor or underexploited crop having great medicinal and food value which belongs to the family Boraginaceae, originates along the Himalayan tract upto 1,500 metres. In-vitro technique is accepted widely to produce virus free plants in a very large number. Production of virus free planting material using meristem culture has been made possible in this crop.
7p nguathienthan8 20-10-2020 15 2 Download
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Morphogenic culture systems are central to crop improvement programs that utilize transgenic and genome editing technologies. We previously reported that CMD2-type cassava (Manihot esculenta) cultivars lose resistance to cassava mosaic disease (CMD) when passed through somatic embryogenesis.
11p vichoji2711 29-04-2020 11 1 Download
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In-vitro propagation is widely used as reliable alternative for true-to-type and quality seed production in sugarcane. The main obstacle under in-vitro propagation is growth of contaminants, resulting in failure of culture establishment that can be minimized by effective surface sterilization method. The sterilization process varied species to species and explants type thus, present study was designed to evaluate the effects of various sterilants on in-vitro culture establishment of sugarcane variety Co 0118 through meristem shoot tip.
8p trinhthamhodang3 12-02-2020 13 1 Download
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Pigeon pea (Cajanus cajan L. Millsp.) is one of the major grain legume crops of the tropics and sub-tropics grown in about 50 countries of Asia, Eastern and Southern Africa and the Caribbean for various uses such as food, fodder and firewood. The present study was conducted to develop and optimize tissue culture independent in planta transformation system in pigeonpea. This system can help in widening genetic base as well as solutions over various biotic and abiotic factors through engineering novel genes across the species. The various transformation parameters viz.
13p nguathienthan2 26-12-2019 11 0 Download
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Studies were carried out for rapid micropropagation of two sugarcane genotypes Co-86032 and CoVC-18061. The explants were surface sterilized with 75% alcohol for 30 minutes using cotton. The cultures were initiated by inoculating shoot apical meristem on MS (Murashige and Skoog, 1962) medium containing 1.0 mg/l kinetin. The multiplication response of two sugarcane genotypes was studied under five levels of 6-Benzylaminopurine (0, 0.5, 1, 1.5 and 2 mg/l) and five levels of kinetin (0, 0.25, 0.5, 1.0 and 1.5 mg/l) in completely randomized design with 5x5x2 factorial treatment combinations.
9p cothumenhmong1 08-12-2019 18 0 Download
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A protocol for direct plantlet regeneration from aerial stem explants of ginger (Zingiber officinale Rosc.) has been developed. Adventitious shoots and roots were regenerated from in planta aerial stem explants of 2 ginger varieties viz. var. ‘Jamaica’ and var. ‘Varada’. Maximum shoot and root regeneration were observed in the cultures containing TDZ and IBA (1:1; 1:0.1 mg L-1) in both varieties.
9p vibasque27 27-03-2019 16 1 Download
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A protocol for production of virus-free Vitis vinifera using meristem culture was developed. Meristems (0.1-0.2 mm) of V. vinifera infected with grapevine fanleaf virus and grape leafroll associated viruses were excised from 1-yearold growing vines.
12p masterbarista 15-01-2019 30 2 Download
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Tuyển tập các báo cáo nghiên cứu về lâm nghiệp được đăng trên tạp chí lâm nghiệp quốc tế đề tài: "Rejuvenation of a 100 yr old giant sequoia (Sequoiadendron giganteum Buchholz) through in vitro meristem culture...
4p toshiba8 11-10-2011 23 1 Download