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Metabolic transformations
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Glutamine synthetase (EC 6.3.1.2) is an enzyme which assimilates ammonium into amino acids. Therefore, GS plays an essential role in the metabolism of nitrogen. In vascular plants, GS is devided into two groups: cytosol - located isoform (GS1) and plastid – located isoform (GS2). Surveys have shown that GS1 gene was used widely to generate transgenic plants. Effects on the growth characteristics of GS1 transgenic plants have been reported in many researches.
7p
vilarry
01-04-2024
6
1
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Ebook "Proteases and cancer: Methods and protocols" explores the role of proteases in various catalytic classes and cellular processes associated with cancer. The chapters in this book cover topics such as encoding proteolytic enzymes, cleavage sites of proteases, functional catalytic domains of MMPs, zymography, angiogenesis and lymphangiogenesis, genetic and epigenetic alterations of metallopeptidases, and methods to target metalloproteinases.
355p
lucchinguyen
28-12-2023
3
2
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Part 1 book "Principles of animal cell culture" includes content: History of development of cell culture, natural surroundings of animal cell, metabolic capabilities of animal cell, simulating natural conditions for growing animal cells, serum and serum free media, cell culture system, commonly used cell lines and their uses, growth kinetics of cells in culture, secondary culture - transformed animal cell.
147p
oursky05
20-09-2023
7
2
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Despite great progress in complex gene regulatory mechanisms in the dynamic tumor microenvironment, the potential contribution of long noncoding RNAs (lncRNAs) to cancer cell metabolism is poorly understood. Death receptor 5 antisense (DR5-AS) is a cisplatin inducible lncRNA whose knockdown modulates cell morphology. However, its effect on cell metabolism is unknown. The aim of this study is to examine metabolic changes modulated by cisplatin and DR5-AS lncRNA in HeLa cells.
14p
lyhuyenthu
31-01-2023
9
2
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The rol oncogenes of Agrobacterium rhizogenes enhance the production of medicinally important compounds in plants and provide a first barrier against the overproduction of reactive oxygen species during biotic and abiotic stress. This study was designed to evaluate the expression of genes involved in biosynthetic pathways and their impact on metabolic contents and environmental stress tolerance in regenerated Ajuga bracteosa Wall. ex Benth. After successful transformation, real-time quantitative PCR confirmed the increased expression (1.94–6.
19p
tudichquannguyet
29-11-2021
13
1
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Root-knot nematodes (RKN), genus Meloidogyne, are plant parasitic worms that have the ability to transform root vascular cylinder cells into hypertrophied, multinucleate and metabolically over-active feeding cells.
16p
viseulgi2711
31-08-2021
6
1
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Cylindrospermopsin is a highly persistent cyanobacterial secondary metabolite toxic to humans and other living organisms. Strain OF001 and A210 are manganese-oxidizing bacteria (MOB) able to transform cylindrospermopsin during the oxidation of Mn2+.
19p
vilichoo2711
23-06-2021
13
1
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Meningiomas are common brain tumours that are usually defined by benign clinical course. However, some meningiomas undergo a malignant transformation and recur within a short time period regardless of their World Health Organization (WHO) grade.
9p
vianrose2711
27-04-2021
5
1
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Phytoremediation includes phytotransformation (transformation of organic contaminants into a less toxic, less mobile, or more stable form), phytodegradation (metabolism of the organic contaminant by the plant enzymes), phytovolatilization (volatilization of organic contaminants through plant leaves), and rhizoremediation.
15p
caygaocaolon11
21-04-2021
15
1
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Chapter 6 - An introduction to metabolism. This chapter presents the following content: The chemistry of life is organized into metabolic pathways, organisms transform energy, the energy transformations of life are subject to two laws of thermodynamics, organisms live at the expense of free energy, ATP powers cellular work by coupling exergonic reactions to endergonic reactions.
34p
tradaviahe19
29-03-2021
8
1
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This chapter presents the following content: An organism's metabolism transform matter and energy, of thermodynamics, The free-energy change of a reaction tells us whether or not the reaction occurs spontaneously, ATP powers cellular work by coupling exergonic reactions to engergonic reactions, enzymes speed up metabolic reactions by lowering energy barriers, regulation of enzyme activity helps control metabolism.
66p
tradaviahe14
27-01-2021
16
4
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Metabolism is generally modeled by directed networks where nodes represent reactions and/or metabolites. In order to explore metabolic pathway conservation and divergence among organisms, previous studies were based on graph alignment to find similar pathways.
9p
vioklahoma2711
19-11-2020
13
2
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Metabolomics has the promise to transform the area of personalized medicine with the rapid development of high throughput technology for untargeted analysis of metabolites. Open access, easy to use, analytic tools that are broadly accessible to the biological community need to be developed.
11p
viconnecticut2711
28-10-2020
15
0
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Stable isotope tracing can follow individual atoms through metabolic transformations through the detection of the incorporation of stable isotope within metabolites. This resulting data can be interpreted in terms related to metabolic flux.
12p
vicolorado2711
23-10-2020
8
1
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Increased glycolytic activity is a hallmark of cancer, allowing staging and restaging with 18F-fluorodeoxyglucose-positron-emission-tomography (PET). Since interim-PET is an important prognostic tool in Hodgkin lymphoma (HL), the aim of this study was to investigate the expression of proteins involved in the regulation of glucose metabolism in the different HL subtypes and their impact on clinical outcome.
7p
vijisoo2711
30-09-2020
5
0
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Thyroid hormones have been shown to regulate breast cancer cells growth, the absence or reduction of thyroid hormones in cells could provoke a proliferation arrest in G0-G1 or weak mitochondrial activity, which makes cells insensitive to therapies for cancers through transforming into low metabolism status.
12p
vijennie2711
29-09-2020
15
1
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One of the hallmarks of cancer is an altered energy metabolism, and here, mitochondria play a central role. Previous studies have indicated that some mitochondrial ribosomal proteins change their expression patterns upon transformation.
11p
vimale2711
25-08-2020
9
1
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Stevia rebaudiana produces sweet-tasting steviol glycosides (SGs) in its leaves which can be used as natural sweeteners. Metabolic engineering of Stevia would offer an alternative approach to conventional breeding for enhanced production of SGs. However, an effective protocol for Stevia transformation is lacking.
16p
vishikamaru2711
29-04-2020
6
0
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Transformation is the process by which the genetic make-up of an organism is altered by the insertion of new gene into its genome. As most of the agronomic traits are polygenic in nature, so the integration of multiple genes is required to manipulate the complex, polygenic metabolic or regulatory pathways, while ensuring their stable inheritance and expression in succeeding generations. There are various methods for multiple gene transformation but co-transformation method has proved more efficient and transplastomic technology can be used whenever have an option.
6p
nguathienthan2
19-12-2019
29
0
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Recently environmental metagenomics are useful methodology to study microbial diversity in the environment as well as functional metabolic genes. This study was also based on metagenomic method to discover antibiotic resistance genes from aquatic environments. To create a metagenomic library, the environmental DNA was extracted from water and sediment sample of Thi Nghe canal, Ho Chi Minh City. Total DNA then was fragmented by sizes of 1-3 kb and inserted in to pUC19 plasmid. After transformation into E.
8p
nguathienthan1
27-11-2019
15
0
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