Metallo-endopeptidase
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The metalloendopeptidase 24.15 (EP24.15) is ubiquitously present in the extracellular environment as a secreted protein. Outside the cell, this enzyme degrades several neuropeptides containing from 5 to 17 amino acids (e.g. gonadotropin releasing hormone, bradykinin, opioids and neuro-tensin). The constitutive secretion of EP24.15 from glioma C6 cells was demonstrated to be stimulated linearly by reduced concentrations of extra-cellular calcium.
15p awards 06-04-2013 26 2 Download
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In the past, protease-substrate finding proved to be rather haphazard and was executed byin vitro cleavage assays using singly selected targets. In the present study, we report the first protease proteomic approach applied to meprin, an astacin-like metalloendopeptidase, to determine physiological substrates in a cell-based system of Madin–Darby canine kidney epithelial cells.
20p galaxyss3 07-03-2013 26 2 Download