Methods of living culture

Ebook "Cellular heterogeneity: Methods and protocols" is essential for anyone struggling with the cellular heterogeneity field, and it connects this new area with the latest advances in innovative techniques combined with practical advice from international experts. The chapters provide a comprehensive overview of cellular heterogeneity analysis techniques, exploring cytometry, imaging and spectroscopy methods, metabolic and molecular biological methods in tracking, as well as chromatin and cell cycle heterogeneity.

363p lucchinguyen 28-12-2023 8 3   Download

Part 1 book "Animal cell culture - Essential methods" includes content: The cell culture laboratory, sterilization, microscopy of living cells, basic techniques and media, the maintenance of cell lines, and safety, development and optimization of serum and protein free culture media.

214p oursky04 14-09-2023 5 3   Download

The microbial community of the built environment (BE) can impact the lives of people and has been studied for a variety of indoor, outdoor, underground, and extreme locations. Thus far, these microorganisms have mainly been investigated by culture-based methods or amplicon sequencing.

17p vigiselle2711 30-08-2021 6 1   Download

This paper explores the nature of English as an International Language from the point of view of the fit between language forms and cultural pragmatics. Some language forms can have different culturalpragmatic roles for different speakers, and some cultural-pragmatic roles can be differently expressed by different speakers. Some cultural-pragmatic roles have no direct linguistic realization in some varieties of EIL.

6p trinhthamhodang8 02-11-2020 12 1   Download

Objective of the research was to validate the potential of Syzygium aromaticum and Kaempheria galanga against biofilm formation and plasmid borne drug resistance exhibited by Pseudomonas spp. Antimicrobial property of S.aromaticum and K.galanga on P.aeruginosa culture was assessed using the agar well diffusion method. Crystal violet assay was employed to determine the percentage inhibition of P.aeruginosa biofilm by the extracts following generation of biofilm on multiwell plates.

7p nguaconbaynhay6 23-06-2020 31 0   Download

The Western hills of Manipur, India are inhabited by tribal people mostly of Zeliangrong tribe and to some extent by Kuki tribe. They had been living in an isolated society with its own socio-cultural pattern and tradition. The seasonal food produce were processed to enhance nutritive value, flavour and above all shelf life to consume in the lean season. The most common method of processing is sun drying and natural fermentation and boiling in the plain water. Lack of or conscious efforts to limit salt uses were evident during the survey.

11p nguathienthan5 04-06-2020 9 0   Download

A new trend is fast emerging in the society all over the world where two adults of opposite sex giving a complete go by to the institution of marriage decide to live-in relationship which almost resembles a marriage. The present study was undertaken to study the Perceptions of Young adults (80 students both males and females) towards Live-in relationship by purposive random sampling method. Self-developed questionnaire was used to measure the perceptions of young adults towards live-in relationship. Twin cities of Hyderabad and Secunderabad were selected for the study.

6p trinhthamhodang1213 02-06-2020 10 0   Download

The poets associated with the L=A=N=G=U=A=G=E School have remarkably borrowed from the Pound-Olson tradition of political poetry and poetics. But, they have also experimented with newer methods and matters that deviate from the tradition. Such continuation and departure from the tradition of the socio-cultural oriented language poets has been investigated here. Their poetic tenets have been examined through the ideas formulated by the outstanding cultural critics; Adorno, Jameson, Bakhtin, Foucault, Lukács, and Benjamin.

16p nguathienthan1 27-11-2019 20 0   Download

Improving cultivability of a wider range of bacterial and archaeal community members, living natively in natural environments and within plants, is a prerequisite to better understanding plant-microbiota interactions and their functions in such very complex systems. Sequencing, assembling, and annotation of pure microbial strain genomes provide higher quality data compared to environmental metagenome analyses, and can substantially improve gene and protein database information.

13p caygaocaolon1 13-11-2019 18 1   Download

Objectives: To compare pregnancy rate, live birth rate between 2 groups 5% O2 and 20% O2. Subjects and method: This is a randomized clinical trial study among 172 patients who were divided into 2 groups: embryos cultured in 5% and 20% oxygen concentrationture in low oxygen concentration.

5p jangni3 20-04-2018 15 1   Download

Yet these commercial outcomes are the tip of the iceberg. What matters most, to those who live or work in a city, as much as its visitors, is its quality of life, of which culture is the essence. Cities offer not just a natural environment, but a built and cultural one. London’s air, rivers and green spaces are a backdrop; it falls to its building and activities to set the stage on which its life plays out. This is why the cultural quality of life of London’s citizens is an object of policy in its own right. London’s cultural environment also...

96p tay_thi_le 19-02-2013 58 7   Download

In recent years, thanks to various global meetings such as the World Toilet Summits and Asia Pacific Toilet Symposiums, the issue of Public Toilets has evolved from being an embarrassing subject to one that is gaining widespread awareness and generating lively discussions. Toilet is culture; we visit the toilet several times a day. To enjoy our lives, we need to develop good toilet culture.

380p tiramisu0908 25-10-2012 71 9   Download

Another of the great and in some ways one of the most remarkable industries of the Orient is that of silk production, and its manufacture into the most exquisite and beautiful fabrics in the world. Remarkable for its magnitude; for having had its birthplace apparently in oldest China, at least 2600 years B. C.; for having been founded on the domestication of a wild insect of the woods; and for having lived through more than four thousand years, expanding until a $1,000,000 cargo of the product has been laid down on our western coast at one time and rushed...

9p asus1122 24-10-2012 52 2   Download

This book explores the diversity of topics related to radiometric dating, with particular emphasis on the method of radiocarbon dating and a cross-check of its results with luminescence measurements. Chemistry and the global carbon cycle is an essential element of life on Earth, because carbon is a component of every living cell, plants and animals. The radioactive isotope 14C was found useful for dating, for which in 1960 Willard Frank Libby received the Nobel Prize in Chemistry.

0p wqwqwqwqwq 06-07-2012 63 6   Download