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Methylation specific PCR
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Nghiên cứu nhằm khảo sát vị trí thiết kế mồi trên trình tự giàu CpG của Alu để sử dụng cho phản ứng real time PCR đặc hiệu methyl hóa (quantitative Methylation Specific PCR-qMSP) cho phép đánh giá được sự thay đổi methyl hóa Alu ở 25 cặp mẫu mô ung thư/liền kề của bệnh nhân ung thư vú tại Việt Nam.
9p
visergeyne
18-06-2024
0
0
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Breast cancer is the most common female tumors arising worldwide, and genetic and epigenetic events are constantly accumulated in breast tumorigenesis. The melastatin-related transient receptor potential 7 channel (TRPM7) is a nonselective cation channel, mainly maintaining Zn2+, Ca2+ and Mg2+ homeostasis. It is also involved in regulating proliferation and migration in various cancers including breast cancer.
10p
vialfrednobel
23-12-2023
5
4
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Part 2 book "PCR Protocols" includes content: Linking emulsion PCR haplotype analysis, the many faces of MLPA, assessing gene specific methylation using HRM-Based analysis, asynchronous PCR, enhanced solid phase PCR for increased loading of amplicon onto solid support, application of blocking oligonucleotides to improve signal to noise ratio in a PCR,...and other contents.
179p
oursky06
17-10-2023
3
1
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In this study, formalin-fixed, paraffin-embedded (FFPE) tissue samples were collected from 30 lung cancer patients and 30 patients suffering from non-cancerous pulmonary diseases. The methylation level of SHOX2 was evaluated in two CpG-riched regions of the promoter by using qMSP.
6p
meyerowitz
25-12-2021
12
0
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In oncology, liquid biopsy is of major relevance from theranostic point of view. The searching for mutations in circulating tumor DNA (ctDNA) in case of colorectal cancers (CRCs) allows the optimization of patient care. In this context, independent of mutation status biomarkers are required for its detection to confirm the presence of ctDNA in liquid biopsies.
12p
vimahuateng
26-11-2021
19
1
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The association between SOX14 and cancer has been reported. The aim of this study was to identify and validate the potential value of SOX14 methylation in the early detection of cervical cancer.
10p
vimahuateng
26-11-2021
8
1
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Epigenetic modulation plays a vital role in regulating the transcriptional machinery of human genome in response to infection. Differential Methylation pattern of the genome especially the retro transposon repetitive elements (RE) which constitutes more than 45% of the genome had been studied to trace the impact of RE methylation in the disease severity.
15p
caygaocaolon11
21-04-2021
18
4
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Our preliminary RNA-Seq data revealed altered expression of small nucleolar RNA host gene 9 (SNHG9) in osteoarthritis (OA) and its reverse correlation with miR-34a, which can regulate chondrocyte apoptosis in rat OA model. This study was therefore carried out to investigate the potential interaction between SNHG9 and miR-34a in OA.
7p
vimariana2711
22-12-2020
8
0
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CST6 promoter is highly methylated in cancer, and its detection can provide important prognostic information in breast cancer patients. The aim of our study was to develop a Methylation-Sensitive High Resolution Melting Analysis (MS-HRMA) assay for the investigation of CST6 promoter methylation.
11p
viirene2711
03-10-2020
10
0
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Quantitative methylation-specific PCR (qMSP) analysis for determining the methylation status of (candidate) tumor suppressor genes has potential as objective and valuable test to triage high-risk human papillomavirus (hrHPV) positive women in cervical screening.
9p
vijisoo2711
30-09-2020
13
0
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O6-methylguanine-DNA methyltransferase (MGMT) promoter methylation is reported to be a prognostic and predictive factor of alkylating chemotherapy for glioblastoma patients. Methylation specific PCR (MSP) has been most commonly used when the methylation status of MGMT is assessed.
11p
vilisa271
22-09-2020
10
0
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Recent studies have shown that miR-372 plays important roles in hepatocellular carcinoma (HCC) progression. However, results have been conflicting regarding its expression levels and role in HCC. Methods: RT-PCR and in situ hybridization was used to evaluate miR-372 expression in HCC tissues and cell lines. The methylation status of neighboring CpG islands upstream of the miR-372 promoter was analyzed by methylation-specific PCR (MSP).
12p
vialabama2711
21-09-2020
8
0
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Interrogation of site-specific CpG methylation in circulating tumor DNAs (ctDNAs) has been employed in a number of studies for early detection of breast cancer (BrCa). In many of these studies, the markers were identified based on known biology of BrCa progression, and interrogated using methyl-specific PCR (MSP), a technique involving bisulfite conversion, PCR, and qPCR.
15p
vikuala271
13-06-2020
10
2
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Using quantitative methylation-specific PCR (QM-MSP) is a promising method for colorectal cancer (CRC) diagnosis from stool samples. Difficulty in eliminating PCR inhibitors of this body fluid has been extensively reported.
8p
viuchiha2711
21-04-2020
11
0
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E. coli is a Gram negative bacteria belonging to enterobacteriacea family. They are commonly found in the intestine of animals and humans. Most E. coli are harmless but some can cause infection. In our study, we isolated E.coli from 100 (50 from sheep and 50 from goats) faecal samples of diarrhoeic as well as healthy sheep. Isolation was done on BHI, MLA and EMB agar. The isolated bacteria suspected for E. coli were subjected to biochemical tests viz catalase, oxidase and IMViC. The isolates confirmed by biochemical tests were further confirmed by genus specific PCR.
7p
cothumenhmong3
22-02-2020
18
0
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The present study included 31 HCC with their ANT, 38 CH and 13 normal hepatic tissue (NHT) samples. In all groups, PM of APC, FHIT, p15, p73, p14, p16, DAPK1, CDH1, RARb, RASSF1A, O6 MGMT was assessed by methylation-specific PCR (MSP). APC and O6-MGMT protein expression was assessed by immunohistochemistry (IHC) in the studied HCC and CH (20 samples each) as well as in a different HCC and CH set for confirmation of MSP results. PM was associated with progression from CH to HCC.
14p
kethamoi1
17-11-2019
22
1
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Nghiên cứu mô tả cắt ngang. Tách chiết cfDNA (DNA tự do) từ mẫu huyết tương của thai phụ. Bài viết trình bày việc ác định sự hiện diện của cffDNA thông qua sự khác biệt trạng thái methyl hóa vùng promoter gen RASSF1A.
8p
viposeidon2711
18-09-2019
28
0
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