Micropropagation medium

In this study the effectiveness of plant growth regulators on micropropagation, total phenol content and metabolic profiles of Melissa officinalis L., important medicinal herb was assessed. The stem segments derived from one-month old in vitro germinated seedlings were used for initial explants. Of the eight different nutrient media tested the most favorable for micropropagation were found to be MP2 (MS medium enriched with 1mg/L BAP and 0.1 mg/L IBA) and MP3 (MS supplemented with 1.5 mg/L BAP and 0.5 mg/L NAA), which yielded 3.25- 4.0 shoots per explant within 4 weeks of culture.

5p mudbound 10-12-2021 16 1   Download

Roses are one of the most popular fl owers in the world because of their beauty, color, aroma as well as their various uses and Red Eden rose is one of the most popular hybrid roses. Results of the study showed the most optimal medium for shoot multiplication, which was MS + 30 g/l of sucrose + 8 g/l of agar supplemented with 0.5 mg/l of BA. The optimal medium for growth and fl owering was ½MS + 30 g/l of sucrose + 8 g/l of agar supplemented with 30 μM of AgNO3 . However, NAA at all tested concentrations did not show any eff ect on in vitro plant growth.

7p nguaconbaynhay12 03-06-2021 23 2   Download

Chickpea is best nutritional crop in leguminous species, and biotic and abiotic stress affects the yield loss around 50 to 60 % .For preventing yield loss from this stress, it is necessary to developed high yield, qualitative variety of chickpea. In vitro methods are best technique for regeneration and development of transgenic variety of chickpea. In this investigation research to determine chickpea in vitro micropropagation was studied in genotypes; of chickpea JG62, JG63 JG315, VIJAY and Bhupdachana with explants immature cotyledonary and embryonic axis.

10p cothumenhmong11 11-05-2021 5 1   Download

Production of bamboo plants by using vegetative propagation is not simple to establish a commercial fields however micropropagation techniques has much ability to achieve the bamboo seedling demands. In vitro cultures of Bambusa balcooa were achieved by using nodal segment as an explant. Eight different combinations of plant growth regulators with MS basal medium were used for shoot induction and multiplication.

7p trinhthamhodang11 27-04-2021 15 2   Download

The micropropagation protocol of tropical, small evergreen, true mangrove, and woody shrub or tree species, Aegiceras corniculatum, has been standardized. Axillary shoot proliferation was induced in vitro from nodal explants excised from 3-4 years field-grown old plant which was then used as explants for the establishment of in vitro cultures. Surface-sterilized explants were cultured on the Murashige and Skoog (MS) basal medium supplemented with different concentrations and a combination of growth regulators.

7p caygaocaolon11 21-04-2021 4 1   Download

The present investigation was carried out to study the influence of antioxidants on in vitro culture of Gliricidia sepium. Soft nodal stem segments were inoculated on MS medium supplemented with desired concentrations plant growth regulator such as cytokinins (BAP/Kinetin) and different antioxidants. Antioxidants viz., activated charcoal, ascorbic acid, citric acid and polyvinylpyrollidone were used to control the accumulation of phenolic compounds in the culture medium and enhance the rate of micropropagation.

6p trinhthamhodang1218 26-02-2021 11 1   Download

In addition, the net photosynthetic rate (Pn) of plants under PA condition was statistically higher and increased over the culture period while Pn of plants under PM condition remained constant. This study demonstrated that MS salts with half concentration of NH4NO3 and photoautotrophic culture method were appropriate for in vitro growth of hydrangea.

11p nguaconbaynhay10 20-02-2021 11 2   Download

An experiment was employed to identify the suitable rooting hormone and a potential hardening medium for gerbera micropropagation techniques. IBA (Indole Butyric Acid) and GA3 (Gibbrelic Acid) are taken for the study of rooting and Sand, Cocopeat, Vermicompost and Vermiculite selected alone or in combinations for the study of hardening in gerbera. Different parameters on root induction were taken similarly morphological and biochemical parameters were studies in hardend plants of gerbera.

9p chauchaungayxua8 03-10-2020 8 1   Download

Banana is one of the important fruit crops and food source for million people in developing countries. In the study explants (Suckers) of two banana cultivars were cultured on Murashige and Skoog (MS) medium supplemented with different concentrations of BAP and Kinetin sole and in combination for shoot initiation and multiplication.

10p trinhthamhodang5 16-05-2020 10 0   Download

Litchi is a very important fruit of the Indian subcontinent and has a great demand in local and international market. But it is a very troublesome plant to cultivate and the current production rate cannot meet the growing demands of litchi. The in vitro techniques can be used to rapidly increase production. The present study was carried out to develop a rapid micropropagation protocol for litchi by studying the effect of medium and the explant used.

10p kethamoi4 18-04-2020 10 1   Download

Present investigation was carried out to determine the optimum level of different plant growth regulators for rapid shoot multiplication, rooting and hardening of micropropagated plantlets of Aloe vera. The experiment was laid out using completely randomized design (CRD) with ten replications during 2018-19. Auxiliary shoot explants were inoculated on MS medium supplemented with various concentrations of cytokinin (BAP 0.5 – 6.0 mg/l) alone and BAP (0.5-6.0 mg/l) with auxin (NAA 0.1 – 0.6) in combination for direct shoot bud proliferation.

12p caygaocaolon4 04-04-2020 21 1   Download

The present study was carried out to monitor somaclonal variations of banana cv. Ney Poovan (AB) during micropropagation using random amplified polymorphic DNA (RAPD). Sword suckers were used as explants and regenerated on MS media supplemented with BAP alone, BAP + Kinetin and TDZ at varying levels of concentrations. The regenerated shoots were again subcultured on MS medium fortified with plant growth regulators at different concentrations. Rooting was obtained on ½ MS medium supplemented with 2.00 mg/l IBA and 2 g/l of activated charcoal.

12p trinhthamhodang4 22-03-2020 15 1   Download

In this study culture mediums were tested to find a suitable commercially relevant production system for lily.

8p 12120609 18-03-2020 18 2   Download

Research in plant biotechnology is playing a crucial role in the production and conservation of plant-based resources globally. The aim of the work was to establish favourable culture medium condition for regeneration and better growth of Tinospora cordifolia explant. Plant Micropropagation has also been used as a tool for the propagation of genetically manipulated superior clones.

8p caygaocaolon2 14-03-2020 13 2   Download

Violar tricolor belongs to Viola genus, Violaceae family, Violar tricolor plant is not only grown as an ornamental plant, but also a medicinal herb. In this study, some of factors affect on the process of micropropagation were investigated. In the experiment of sterilization explants, seed germination and shoot formation ability of the Violar tricolor L. reached the best result when they were sterilized by 0.1% HgCl2 for 1 minute.

8p bangchungthep4 09-03-2020 32 1   Download

Papaya is one of the few important fruits which flowers and yield throughout the year. Though a polygamous species with three basic types viz., staminate, pistillate and bisexual, its cultivation with seed propagation is hindered by problems due to vide variability in sex expression and fruit characters. In such point of view, propagation through asexual method could be a better alternative. The micropropagation will substitute method of propagation by cutting or grafting would be of great boon to papaya industry. The procedure for in vitro propagation of papaya (Carica papaya) cv.

6p quenchua2 15-12-2019 17 0   Download

Micropropagation of Indian Blackberry (Syzygium cumini L.) species locally called as Jamun, has always been a challenging task. In India, Jamun tree is an underutilized minor fruit crop species possessing a high medicinal and nutritional value. An efficient protocol for rapid shoot proliferation of Jamun (cv. Rajamun) from nodal segment of locally grown mature trees has been developed. Experiments were conducted at Biotech Centre, Dr. PDKV, Akola during 2016-18. The Lloyd and McCown woody plant medium (WPM) used as a basal media supplemented with growth regulators at varying concentrations.

6p kethamoi2 14-12-2019 13 0   Download

The results showed that protocorms were formed from seeds culture on the ½ MS medium supplemented with 10% potatoes extract within 8 weeks. Protocorms which were cultured on the ½ MS medium containing 30 g/l sucrose, 0.5 g/l AC, 7 g/l agar, 0.1 mg/l NAA and 1 mg/l BA were optimal for PLB formation (8.67 PLBs/sample) after 8 weeks culture. Protocorms converted into normal plants with well-developed shoots and roots on the ½ MS medium supplemented with 20 g/l sucrose, 10% potatoes extract, 0.5 g/l AC, and 7 g/l agar after about 90 days.

8p slimzslimz 08-12-2019 12 1   Download

Studies were carried out for rapid micropropagation of two sugarcane genotypes Co-86032 and CoVC-18061. The explants were surface sterilized with 75% alcohol for 30 minutes using cotton. The cultures were initiated by inoculating shoot apical meristem on MS (Murashige and Skoog, 1962) medium containing 1.0 mg/l kinetin. The multiplication response of two sugarcane genotypes was studied under five levels of 6-Benzylaminopurine (0, 0.5, 1, 1.5 and 2 mg/l) and five levels of kinetin (0, 0.25, 0.5, 1.0 and 1.5 mg/l) in completely randomized design with 5x5x2 factorial treatment combinations.

9p cothumenhmong1 08-12-2019 18 0   Download

A simple and efficient protocol for the clonal micropropagation of Ziziphus spina-christi (L.) Desf., a multipurpose native tree species highly adapted to the harsh environmental conditions of Kuwait, has been established using shoot tips and stem nodal segments as explants. The explants were cultured on Murashige and Skoog (MS) basal medium with and without growth regulators.

5p viminotaur2711 30-10-2019 14 0   Download