Microsatellite marker based DNA fingerprinting

Bhakerwal and Pashmina are local Kashmiri goat breeds usually reared by nomadic people and people of Changthang region of Ladakh respectively. They play promising role in alleviating poverty of this far flung region of the country. Adequate breeding and research have been done in these animals to increase their population and quality but parentage analysis and pedigrees record keeping has been a limitation since long time.

4p cothumenhmong9 18-01-2021 11 2   Download

A set of 45 polymorphic microsatellite markers, representing markers for almost each chromosome, was used for the assessment of genetic diversity in 35 genotypes of bread wheat. A total of 127 alleles were detected which ranged from 2 to 6 with an average allele number of 2.82 per locus. The PIC value of the 45 SSRs ranged from 0.08 to 0.50 with an average PIC of 0.30. Resolving power and Marker index of all 45 markers varied from 0.17 to 2.82 and 0.16 to 1.52, respectively.

11p quenchua6 15-06-2020 7 1   Download