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Mitochondrial concentration analysis
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This study aimed to estimate the effect of three concentrations (75 µg/mL, 150 µg/mL and 300 µg/mL) of the European raspberry (Rubus iadeus) on the selected quality parameters of bovine spermatozoa after 0h, 2h, and 24h of in vitro culture. Sperm motility was evaluated using the Computer-assisted sperm analysis (CASA) system. The mitochondrial activity was detected by the metabolic (MTT) assay.
6p
mudbound
10-12-2021
11
0
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Two hundred and forty (240) numbers of day old broiler straight run chicks were wing banded, weighed and randomly allotted to 5 groups 6 replicates of eight chicks each based on the body weight. The treatments were, Basal diet without CoQ10 supplementation, Low energy diet without CoQ10 supplementation, Low energy diet with 20mg of CoQ10 /kg diet, Low energy diet with 40mg of CoQ10 /kg diet, Low energy diet with 60mg of CoQ10 /kg diet. Muscle total lipid was estimated gravimetrically by using Folch method of lipid extraction.
7p
caygaocaolon7
18-09-2020
9
0
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Fruit flies under Tephritidae are one of the diverse and agriculturally important insects. It needs reliable taxonomic procedures to resolve species complexes and genetic structure analysis to effectively control this pest. Little attention has been given to the analysis of genetic diversity in South Indian populations. By using mitochondrial cytochrome oxidase I and II (cox1 and cox2), the genetic analysis of B. dorsalis from eight locations of major fruit growing areas in western parts of Tamil Nadu were investigated.
9p
trinhthamhodang3
12-02-2020
22
0
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Liver total glycogen, mitochondrial protein and CoQ10 Analysis in CoQ10 supplemented broiler chicken
A Pilot study was conducted to find out the level of CoQ10 inclusion in broiler diet to bring about better performance and conducted during the period from November to December. Fifty numbers of day old broiler straight run chicks were wing banded, weighed and randomly allotted to five groups of ten chicks each based on the body weight. The treatments include, 20mg, 40mg, 60mg, 80mg, 100mg of CoQ10/kg diet.
7p
kethamoi2
15-12-2019
5
0
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This review provides a comparative overview of recent developments in the modelling of cellular calcium oscilla-tions. A large variety of mathematical models have been developed for this wide-spread phenomenon in intra- and intercellular signalling. From these, a general model is extracted that involves six types of concentration variables: inositol 1,4,5-trisphosphate (IP3), cytoplasmic, endoplasmic reticulum and mitochondrial calcium, the occupied binding sites of calcium buffers, and the fraction of active IP3 receptor calciumrelease channels....
23p
research12
29-04-2013
26
2
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3,3¢-Dipropyl-2,2¢-thiadicarbocyanine iodide [DiS-C3 (5)], often used as a tracer dye to assess the mitochondrial mem-brane potential, was investigated in detail regarding its effects on the structure and function of isolated mitochon-dria. As reported previously, DiS-C3(5) had an inhibitory effect on NADH-driven mitochondrial electron transfer. On the contrary, in the presence of inorganic phosphate, DiS-C3(5) showed dose-dependent biphasic effects on mito-chondria energized by succinate. At higher concentrations, such as 50lM,DiS-C3(5) acceleratedmitochondrial oxygen consumption. ...
0p
awards
05-04-2013
34
1
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Injury of liver by ischaemia crucially involves mitochondrial damage. The role of Ca 2+ in mitochondrial damage is still unclear. We investigated the effect of low micromolar Ca 2+ concentrations on respiration, membrane permeability, and antioxidative defence in liver mitochondria exposed to hypoxia⁄reoxygenation. Hypoxia⁄reoxygenation caused decrease in state 3 respiration and in the respiratory control ratio.
9p
dell39
27-03-2013
40
2
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Diverse changes have been described in mitochondria of apoptotic cells: the phospholipid content is modified, ceramide and GD3 concentrations increase, the cristae structure is modified, and nonresident proteins are recruited into the mitochondrial membranes. In particular, Bax, a Bcl-2 family member protein, moves from the cytosol to the mitochondria, inducing cytochrome c release.
10p
viettel02
20-02-2013
29
2
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