Mitochondrial genes

Evaluate genetic diversity of Lien Minh chickens based on sequences of mitochondrial DNA D-loop region; determine the genetic relationship and the origin of Lien Minh chickens; identif genetic relationships between candidate genes and egg yield traits in Lien Minh chickens.

27p kequaidan7 01-09-2020 31 3   Download

Comparative analyses of chloroplast and mitochondrial genomes have shown that organelle genomes in bryophytes evolve slowly. However, in contrast to seed plants, the organellar genomes are yet poorly explored in bryophytes, especially among liverworts.

12p vishikamaru2711 29-04-2020 14 1   Download

In this paper the individual markers (COI and 18S rRNA) were successfully used to identify some sponge taxa at the species level. The obtained results showed the congruence of molecular taxonomy using two independent markers. However, our study showed that a combination of the two markers provided more information and supported better for sponge identification.

10p shiwo_ding7 05-06-2019 32 3   Download

Mitochondrial functional and structural impairment and generation of oxidative stress have been implicated in aging, various diseases and chemotherapies. This study analyzed azidothymidine (AZT)-caused failures in mitochondrial functions, in redox regulation and activation of the HIV-1 gene expression. We monitored intracellular concentrations of ATP and glutathione (GSH) as the indicators of energy production and redox conditions, respectively, during the time-course experiments with U937 and MOLT4 human lymphoid cells in the presence of AZT (0.05 mgÆmL)1) or H2O2 (0.01 mM) for 15–25 days.

7p system191 01-06-2013 42 3   Download

Mitochondrial uncoupling protein 1 (UCP1) is a speci®c marker of multilocular brown adipocytes. Ectopic UCP1 in white fat of aP2-Ucp1micemitigates development of obesity byboth, increasing energy expenditure anddecreasingin situ lipogenesis. In order to further analyse consequences of respiratory uncoupling inwhite fat, the e€ects of the ectopic UCP1 on the morphology of adipocytes and biogenesis of mitochondria in these cells were studied.

10p research12 29-04-2013 40 4   Download

Termination of mitochondrial (mt) H-strand transcription in mammalian cells occurs at two distinct sites on the genome. The first site of termination, referred to as mt-TERM occurs beyond the 16 S rRNA gene. However, the second and final site of termination beyond the tRNA Thr gene remains unclear. In this study we have characterized the site of termination of the polycistronic distal gene transcript beyond the D-loop region, immedi-ately upstream of the tRNA Phe gene.

13p tumor12 20-04-2013 42 3   Download

The effects of physiologically relevant hypoxia on the catalytic activity of cytochromecoxidase (CytOX), mito-chondrial gene expression, and both nuclear and mito-chondrial encoded CytOX mRNA levels were investigated in murine monocyte macrophages, mouse C2C12 skeletal myocytes and rat adrenal pheochromocytoma PC12 cells. Our results suggest a coordinated down regulation of mito-chondrial genome-coded CytOX I and II and nuclear genome-codedCytOX IV andVbmRNAs during hypoxia.

9p tumor12 20-04-2013 34 2   Download

Whereas the majority of disease-related mitochondrial DNA mutations exhibit significant biochemical and clinical heterogeneity, mutations within the mitochondrially encoded human cytochrome b gene (MTCYB) are almost exclusively associated with isolated complex III deficiency in muscle and a clinical presentation involving exercise intolerance.

10p fptmusic 11-04-2013 35 3   Download

Membrane-associated calcium-independent phospholipase A2c(iPLA2c) contains four potential in-frame methionine start sites (Mancuso, D.J. Jenkins, C.M. & Gross, R.W. (2000)J. Biol. Chem.275, 9937–9945), but the mechanisms regulating the types, amount and subcellular localization of iPLA2c in cells are incompletely understood.

16p awards 05-04-2013 55 3   Download

Mitochondrial biogenesis is a complex and highly regulated process that requires the controlled expression of hundreds of genes encoded in two separated genomes, namely the nuclear and mitochondrial genomes. To identify regulatory proteins involved in the transcriptional control of key nuc-lear-encoded mitochondrial genes, we have performed a detailed analysis of the promoter region of theasubunit of the Drosophila melanogasterF1F0 ATP synthase complex.

11p awards 05-04-2013 42 5   Download

The cytochromebc1 complex of the yeastSaccharomyces cerevisiae is composed of 10 different subunits that are assembledas a symmetrical dimer in the innermitochondrial membrane. Three of the subunits contain redox centers and participate in catalysis, whereas little is known about the function of the seven supernumerary subunits.

10p dell39 03-04-2013 56 4   Download

Mitochondrial fatty acid oxidation deficiencies are due to genetic defects in enzymes of fatty acidb-oxidation and transport proteins.Genetic defects have been identified in most of the genes where nearly all types of sequence vari-ations (mutation types) have beenassociatedwithdisease.In this paper, we will discuss the effects of the various types of sequence variations encountered and review current know-ledge regarding the genotype–phenotype relationship, espe-cially in patients with acyl-CoA dehydrogenase deficiencies where sufficient material exists for a meaningful discussion....

13p dell39 03-04-2013 49 3   Download

The expression of the colonic mitochondrial 3-hydroxy 3-methyl glutaryl CoA (mHMGCoA) synthase,a key con-trol site of ketogenesis from butyrate,is lower in germ-free (GF) than in conventional (CV) rats. In contrast,the activity of glutaminase is higher. The objective of this study was to investigate whether the intestinal flora can affect gene expression through short chain fatty acid (SCFA) and butyrate production. GF rats were inoculated with a con-ventional flora (Ino-CV) orwitha bacterial strainproducing butyrate (Clostridium paraputrificum,Ino-Cp) or not (Bifidobacterium breve,Ino-Bb).

9p dell39 03-04-2013 28 4   Download

Peroxiredoxins belong to a family of antioxidant proteins that neutralize reactive oxygen species. One member of this family, peroxiredoxin I (PRDX1), suppresses DNA oxidation. Peroxiredoxin V (PRDX5) has been cloned as a transcriptional corepressor, as a peroxisomal⁄mitochondrial antioxidant protein, and as an inhibitor of p53-dependent apoptosis.

11p inspiron33 26-03-2013 46 4   Download

The mitochondrial respiratory complex II, or succinate:ubiquinone oxido-reductase, is an integral membrane protein complex in both the tricarboxy-lic acid cycle (Krebs cycle) and aerobic respiration. The gene sequences of each complex II subunit were measured by RT-PCR. N-terminal sequen-cing work was performed to identify the mitochondrial targeting signal peptide of each subunit.

6p galaxyss3 21-03-2013 48 3   Download

TheycaC-related gene, ycaCR, is uncharacterized, and has no assigned function to date. Here we clearly showed that the ycaC-related gene from the amphioxus Branchiostoma belcheri, BbycaCR, coded for a novel mem-ber of the isochorismatase superfamily, which is mainly localized in the mitochondrial fraction.

9p galaxyss3 07-03-2013 46 4   Download

The finding of a genome-wide oscillation in transcription that gates cells into S phase and coordinates mitochondrial and metabolic functions has altered our understanding of how the cell cycle is timed and how stable cellular phenotypes are maintained.

13p media19 06-03-2013 37 4   Download

Peroxiredoxin V (PRDX5) is a member of the family of mammalian pro-teins that neutralize reactive oxygen species. The PRDX5gene is constitu-tively expressed at a high level in many human tissues, but functional elements of its promoter responsible for a high basal activity in the absence of oxidative stress have still not been identified.

11p media19 05-03-2013 42 2   Download

The mechanisms underlying increased cardioprotection in younger female mice are unclear. We hypothesized that serine-threonine protein kinase (protein kinase B; Akt) triggers a metabolic gene switch (decreased fatty acids, increased glucose) in female hearts to enhance mitochondrial bio-energetic capacity, conferring protection against oxidative stress.

7p media19 04-03-2013 22 3   Download

A cDNA corresponding to theAt3g26690 3 gene, which encodes a Nudix protein (AtNUDT13) with predicted mitochondrial localization, was iso-lated from an Arabidopsis thalianalibrary. The 202 amino acid AtNUDT13 polypeptide was overexpressed inEscherichia coliand purified to homo-geneity.

9p media19 04-03-2013 21 2   Download