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Modify genomic DNA
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The translation of personal genomics to precision medicine depends on the accurate interpretation of the multitude of genetic variants observed for each individual. However, even when genetic variants are predicted to modify a protein, their functional implications may be unclear.
10p
vioraclene
31-03-2024
4
2
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Nuclease-based technologies have been developed that enable targeting of specific DNA sequences directly in the zygote. These approaches provide an opportunity to modify the genomes of inbred mice, and allow the removal of strain-specific mutations that confound phenotypic assessment.
12p
vioraclene
31-03-2024
4
2
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Hox transcription factors are master regulators of animal development. Although highly conserved, they can contribute to the formation of novel biological characteristics when modified, such as during the generation of hybrid species, thus potentially serving as species-specific molecular markers.
13p
vihagrid
30-01-2023
10
3
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DNA methylation is a heritable epigenetic mark, enabling stable but reversible gene repression. In mammalian cells, DNA methyltransferases (DNMTs) are responsible for modifying cytosine to 5-methylcytosine (5mC), which can be further oxidized by the TET dioxygenases to ultimately cause DNA demethylation.
15p
vigalileogalilei
27-02-2022
17
1
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The three-dimensional (3D) organization of chromosomes is linked to epigenetic regulation and transcriptional activity. However, only few functional features of 3D chromatin architecture have been described to date.
15p
vigalileogalilei
27-02-2022
13
1
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DNA methylation plays important roles in determining cellular identity, disease, and environmental responses, but little is known about the mechanisms that drive methylation changes during cellular differentiation and tumorigenesis.
12p
vigalileogalilei
27-02-2022
14
1
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Vertebrate DNA is subjected to epigenetic base modifications that have been thought to be limited to methylated and other modified forms of cytidine. A recent study shows that methylation of adenine to form N6 -methyladenine is a rare but readily detectable modification that can be mapped to distinct genomic sites in vertebrates.
4p
viaristotle
29-01-2022
13
0
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Conventional techniques for single-base resolution mapping of epigenetic modifications of DNA such as 5-hydroxymethylcytosine (5hmC) rely on the sequencing of bisulfite-modified DNA. Here we present an alternative approach called SCL-exo which combines selective chemical labeling (SCL) of 5hmC in genomic DNA with exonuclease (exo) digestion of the bead-trapped modified DNA molecules.
12p
viaristotle
29-01-2022
15
0
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Advancing age progressively impacts on risk and severity of chronic disease. It also modifies the epigenome, with changes in DNA methylation, due to both random drift and variation within specific functional loci.
14p
viaristotle
29-01-2022
6
0
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Direct analogs of chemically modified bases that carry important epigenetic information, such as 5-methylcytosine (m5C)/5-methyldeoxycytosine (5mC), 5-hydroxymethylcytosine (hm5C)/5-hydroxymethyldeoxycytosine (5hmC), and N6 -methyladenosine (m6A)/N6 -methyldeoxyadenosine (6mA), are detected in both RNA and DNA, respectively.
21p
viarchimedes
26-01-2022
12
0
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Bread wheat has a large complex genome that makes whole genome resequencing costly. Therefore, genome complexity reduction techniques such as sequence capture make re-sequencing cost effective. With a highquality draft wheat genome now available it is possible to design capture probe sets and to use them to accurately genotype and anchor SNPs to the genome.
12p
vibeauty
23-10-2021
15
2
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Genomic islands (GIs) are inserts of foreign DNA that have potentially arisen through horizontal gene transfer (HGT). There are evidences that GIs can contribute significantly to the evolution of prokaryotes. The acquisition of GIs through HGT in eukaryotes has, however, been largely unexplored. In this study, the previously developed GI prediction tool, SeqWord Gene Island Sniffer (SWGIS), is modified to predict GIs in eukaryotic chromosomes.
9p
vibeauty
23-10-2021
8
0
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Mitochondrial DNA (mtDNA) copy number has been found associated with multiple diseases, including cancers, diabetes and so on. Both environmental and genetic factors could affect the copy number of mtDNA. However, limited study was available about the relationship between genetic variants and mtDNA copy number. What’s more, most of previous studies considered only environmental or genetic factors. Therefore, it’s necessary to explore the genetic effects on mtDNA copy number with the consideration of PM2.5 exposure and smoking.
9p
vitzuyu2711
29-09-2021
6
1
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The degradation of epigenetic control with age is associated with progressive diseases of ageing, including cancers, immunodeficiency and diabetes. Reduced caloric intake slows the effects of ageing and agerelated disease in vertebrates and invertebrates, a process potentially mediated by the impact of caloric restriction on epigenetic factors such as DNA methylation.
11p
visilicon2711
20-08-2021
4
1
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Genome-scale pooled CRISPR screens are powerful tools for identifying genetic dependencies across varied cellular processes. The vast majority of CRISPR screens reported to date have focused exclusively on the perturbation of protein-coding gene function.
15p
vijeeni2711
24-07-2021
1
0
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Traditionally, generation of new plants with improved or desirable features has relied on laborious and timeconsuming breeding techniques. Genome-editing technologies have led to a new era of genome engineering, enabling an effective, precise, and rapid engineering of the plant genomes.
12p
vijichea2711
28-05-2021
11
1
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Different methods available for extraction of plant genomic DNA suffers from one or more drawbacks including compromised quality, quantity and many more. The extraction of high-quality DNA Simarouba glauca DC is notoriously troublesome due to the high contents of polysaccharides and different secondary metabolites. Herein, we aimed to develop a modified CTAB extraction method to isolate DNA from tissues containing high levels of polysaccharides.
12p
chauchaungayxua11
23-03-2021
15
2
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Genome editing is a technique in which changes are made in the DNA of any organism. A nuclease promotes breaks in the DNA at a specific sequence which is repaired by several mechanisms. It is one of the efficient technologies which enable us to change and edit genome of any organism precisely and accurately. Multiple genome editing technologies have been employed, including zinc finger nucleases, mega nucleases, clustered regularly interspaced short palindromic repeats (CRISPR) along with Cas9 protein and transcription activator-like effector nucleases to modify genome of an organism.
9p
trinhthamhodang1218
26-02-2021
13
3
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Since their first commercialization, the diversity of taxa and the genetic composition of transgene sequences in genetically modified plants (GMOs) are constantly increasing. To date, the detection of GMOs and derived products is commonly performed by PCR-based methods targeting specific DNA sequences introduced into the host genome.
14p
viwyoming2711
16-12-2020
10
0
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A variety of DNA binding proteins are involved in regulating and shaping the packing of chromatin. They aid the formation of loops in the DNA that function to isolate different structural domains.
12p
vikentucky2711
24-11-2020
11
1
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