Molecular assay

Glioblastoma multiforme (GBM) is the most aggressive brain cancer with a poor prognosis. Therefore, the correlative molecular markers and molecular mechanisms should be explored to assess the occurrence and treatment of glioma.WB and qPCR assays were used to detect the expression of CXCL5 in human GBM tissues.

15p vishanshan 27-06-2024 1 1   Download

Ebook "DNA damage detection in situ, ex vivo, and in vivo: Methods and protocols" presents all major assays used in molecular and cell biology for the labeling of DNA damage in situ, ex vivo, and in vivo. It brings together recently introduced techniques, as well as those established earlier, which detect and quantify DNA damage at the scales ranging from subcellular to the level of a whole, living organism.

328p dongmelo 27-05-2024 6 2   Download

Ebook "Cell–Cell interactions: Methods and protocols" is a versatile collection of readily reproducible cell-cell interaction assays for uncovering cellular interactions at the molecular level, both in vitro and in vivo. The protocols cover a diverse set of cell-cell interaction models in both normal and pathological states, are readily adaptable to nearly any cell type and organ system, and include primary data and outcome analysis.

306p dongmelo 27-05-2024 3 3   Download

Ebook "The molecular mechanisms of axenfeld-rieger syndrome" presents detailed information and clinical descriptions and diagnosis of ARS, phenotypical descriptions of patients with ARS, molecular aspects of ARS and gene structure/function studies; describes state-of-the-art scientific methods used to assay genes, gene products and structure/function activities of transcription factors;...

115p tracanhphuonghoa1007 22-04-2024 4 2   Download

Ebook "Microfluidic methods for molecular biology" covers the state-of-the-art research on molecular biology assays and molecular techniques enabled or enhanced by microfluidic platforms. Topics covered include microfluidic methods for cellular separations and single cell studies, droplet-based approaches to study protein expression and forensics, and microfluidic in situ hybridization for RNA analysis.

382p tracanhphuonghoa1007 22-04-2024 2 1   Download

The 2015 American College of Medical Genetics and Genomics (ACMG) and the Association for Molecular Pathology (AMP) guidelines for clinical sequence variant interpretation state that “well-established” functional studies can be used as evidence in variant classification.

19p vibransone 28-03-2024 3 1   Download

The American College of Medical Genetics and Genomics (ACMG)/Association for Molecular Pathology (AMP) clinical variant interpretation guidelines established criteria for different types of evidence. This includes the strong evidence codes PS3 and BS3 for “well-established” functional assays demonstrating a variant has abnormal or normal gene/protein function, respectively.

12p vibransone 28-03-2024 3 2   Download

Variants of uncertain significance represent a massive challenge to medical genetics. Multiplexed functional assays, in which the functional effects of thousands of genomic variants are assessed simultaneously, are increasingly generating data that can be used as additional evidence for or against variant pathogenicity.

11p vibransone 28-03-2024 2 2   Download

Multidrug-resistant (MDR) Mycobacterium tuberculosis complex strains not detected by commercial molecular drug susceptibility testing (mDST) assays due to the RpoB I491F resistance mutation are threatening the control of MDR tuberculosis (MDR-TB) in Eswatini.

11p vibransone 28-03-2024 3 2   Download

The increasing affordability of DNA sequencing has allowed it to be widely deployed in pathology laboratories. However, this has exposed many issues with the analysis and reporting of variants for clinical diagnostic use.

16p vioraclene 31-03-2024 3 2   Download

Cell-free DNA (cfDNA) profiling is increasingly used to guide cancer care, yet mutations are not always identified. The ability to detect somatic mutations in plasma depends on both assay sensitivity and the fraction of circulating DNA in plasma that is tumor-derived (i.e., cfDNA tumor fraction).

15p vibransone 28-03-2024 3 2   Download

Part 1 book "Cryptosporidium - Methods and protocols" includes content: Cryptosporidium diagnostic assays - microscopy; cryptosporidium diagnostic assays - molecular detection; detection of cryptosporidium recovered from large volume water samples using dead end ultrafiltration; cryptosporidium oocyst purification using discontinuous gradient centrifugation; measuring cryptosporidium serologic responses by multiplex bead assay,... and other contents.

210p muasambanhan05 16-01-2024 2 0   Download

Ebook "Early, rapid and sensitive veterinary molecular diagnostics - Real time PCR applications" gives a comprehensive account of the practical aspects of Real time PCR and its application to veterinary diagnostic laboratories. The optimisation of assays to help diagnose livestock diseases is stressed and exemplified through assembling standard operating procedures from many laboratory sources. Theoretical aspects of PCR are dealt with as well as quality control features necessary to maintain an assured testing system.

319p tachieuhoa 28-01-2024 4 2   Download

Ebook "PCR for clinical microbiology: An Australian and international perspective" provides detailed and easy step by step protocols for diagnostic assays in the field of molecular microbiology; provides a valuable overview of molecular diagnostics in infectious diseases; medical criteria for choice of molecular test; guide to set up a clinical molecular diagnostic laboratory;...

420p tachieuhoa 28-01-2024 3 2   Download

Ebook "Cancer stem cells: Methods and protocols" comprehensive collection of methods, protocols, and procedures used for the identification, characterization, and selection of cancer stem cells. These include surface marker expression, side population, spheres formation, ALDH activity, and numerous others.

224p lucchinguyen 28-12-2023 5 2   Download

Molecular subgrouping of medulloblastoma has become important due to its impact on risk group stratification. Immunohistochemistry (IHC) has been widely used but it has innate limitations. The NanoString assay has been proposed as an alternative method.

11p vileonardodavinci 23-12-2023 6 2   Download

Part 2 book "Control of poultry mites" includes content: The testing of antibodies raised against poultry red mite antigens in an in vitro feeding assay; preliminary screen for vaccine candidates; endosymbiotic bacteria living inside the poultry red mite (Dermanyssus gallinae); molecular phylogenetic assessment of host range in five Dermanyssus species, monitoring of Dermanyssus gallinae in free-range poultry farms,...and other contents.

98p oursky10 04-12-2023 3 2   Download

Ovarian cancer is one of the most lethal cancers in women because it is often diagnosed at an advanced stage. The molecular markers investigated thus far have been unsatisfactory. We performed whole-exome sequencing on the human ovarian cancer cell lines 3AO and ES2 and the normal ovarian epithelial cell line IOSE-80.

13p vischultz 20-10-2023 1 1   Download

Part 1 book "Molecular diagnostic PCR handbook" includes content: Background; PCR – the basic reaction; amplicon detection, analysis and PCR evaluation; limitations and troubleshooting, specific types of PCR assays.

118p oursky06 17-10-2023 5 1   Download

Part 2 book "Molecular diagnostic PCR handbook" includes content: The PCR laboratory, disease diagnosis using PCR specific procedures for important veterinary pathogens, the detection of Mycoplasma, contagious bovine pleuropneumonia and contagious caprine pleuropneumonia, using a PCR assay, the detection of Rift valley fever virus using a PCR assay, the detection of lumpy skin disease virus using a PCR assay.

207p oursky06 17-10-2023 5 1   Download