Molecular features

The molecular data of D2-D3 of 28S rRNA region, morphometrics, and morphological features of second-stage juveniles, males, and females in the present study have confirmed that the root-knot nematode recovered from pomelo in Vietnam belongs to M. enterolobii. To the best of our knowledge, this represents the first report of M. enterolobii infecting pomelo, providing new insight into the host status of this important pest.

10p dianmotminh02 03-05-2024 5 2   Download

Advanced gastric cancer (AGC) is a disease with poor prognosis due to the current lack of effective therapeutic strategies. Immune checkpoint blockade treatments have shown effect responses in patient subgroups but biomarkers remain challenging. Traditional classifcation of gastric cancer (GC) is based on genomic profiling and molecular features.

13p vielonmusk 21-01-2022 10 1   Download

Glioblastoma (GBM) can be divided into subtypes according to their genomic features, includ‑ ing Proneural (PN), Neural (NE), Classical (CL) and Mesenchymal (ME). However, it is a difficult task to unify various genomic expression profiles which were standardized with various procedures from different studies and to manually classify a given GBM sample into a subtype.

13p vielonmusk 21-01-2022 16 1   Download

The auxin response factor (ARF) gene family is involved in plant development and hormone regulation. Although the ARF gene family has been studied in some plant species, its structural features, molecular evolution, and expression profiling in Brachypodium distachyon L. are still not clear.

15p vishikamaru2711 29-04-2020 31 2   Download

Oceans provide enormous and diverse space for marine life. Invertebrates are conspicuous inhabitants in certain zones such as the intertidal; many are soft- bodied, relatively immobile and lack obvious physical defenses. These animals frequently have evolved chemical defenses against predators and overgrowth by fouling organisms. Marine animals may accumulate and use a variety of toxins from prey organisms and from symbiotic microorganisms for their own purposes. Thus, toxic animals are particularly abundant in the oceans.

23p uocvong01 24-09-2015 63 7   Download

We used parameter scanning to emulate changes to the limitingrate for steps inafittedmodel of glucose-derepressed yeast glycolysis. Three flux-control regimes were observed, two of which were under the dominant control of hexose transport, in accordance with various experimental studies andothermodel predictions.

11p research12 23-04-2013 41 2   Download

NMR spectroscopy and X-ray crystallography have provi-ded important insight into structural features of phenyl-alanine hydroxylase (PAH) and tyrosine hydroxylase (TH). Nevertheless, significant problems such as the substrate specificity of PAHand the different susceptibility of TH to feedback inhibition by L-3,4-dihydroxyphenylalanine (L-DOPA) compared with dopamine (DA) remain unre-solved.

11p tumor12 20-04-2013 51 3   Download

Inorganic pyrophosphatase (PPase) is an important enzyme that catalyzes the hydrolysis of inorganic pyro-phosphate (PPi)intoortho-phosphate (P i ). We report here the molecular cloning and characterization of a gene encoding the soluble PPase of the roundworm Ascaris suum. The predicted A. suumPPase consists of 360 amino acids with a molecular mass of 40.6 kDa and a pI of 7.1.

13p fptmusic 16-04-2013 35 3   Download

To elucidate the structural features of the mussel defensin MGD1 required for antimicrobial activity, we synthesized a series of peptides corresponding to the main known secon-dary structures of the molecule and evaluated their activity towards Gram-positive and Gram-negative bacteria, and filamentous fungi. We found that the nonapeptide corres-ponding to residues 25–33 of MGD1 (CGGWHRLRC) exhibited bacteriostatic activity once it was cyclized by a non-naturally occurring disulfide bridge.

9p fptmusic 16-04-2013 47 2   Download

A molecular understanding of volatile anesthetic mechanisms of action will require structural descriptions of anesthetic–protein complexes. Porcine odorant binding protein is a 157 residue member of the lipocalin family that features a large b-barrel internal cavity (515 ± 30 A ˚ 3 ) lined predomin-antly by aromatic and aliphatic residues. Halothane binding to theb-barrel cavity was determined using fluorescence quenching of Trp16, and a com-petitive binding assay with 1-aminoanthracene.

0p awards 05-04-2013 18 2   Download

Experimental autoimmune encephalomyelitis can be induced in susceptible animals by immunodominant deter-minants of myelin basic protein (MBP). To characterize the molecular features of antigenic sites important for designing experimental autoimmune encephalomyelitis suppressing molecules, we report structural studies, based on NMR experimental data in conjunction with molecular dynamic simulations, of the potent linear dodecapeptide epitope of guinea pig MBP, Gln74-Lys75-Ser76-Gln77-Arg78-Ser79-Gln80-Asp81-Glu82-Asn83-Pro84-Val85 [MBP(74–85)], and its antagonist analogue Ala81MBP(74–85)....

15p awards 05-04-2013 54 3   Download

Circular dichroism (CD) spectra of two major hemoglobin components (Hb), HbI and HbIV, from Oncorhyncus mykiss(formerly Salmo irideus) trout were evaluated in the range 250–600 nm. HbI is characterized by a complete insensitivity to pH changes, while HbIV presents the Root effect. Both reduced [iron(II) or oxy] and oxidized (met) forms of the two proteins were studied at different pHs, 7.8 and 6.0, to obtain information about the pH effects on the structural features of these hemoglobins.

9p dell39 03-04-2013 37 4   Download

Chronic obstructive pulmonary disease is a major clinical disorder usually associated with cigarette smoking. A central feature of chronic obstructive pulmonary disease is inflammation coexisting with an abnormal protease/anti-protease balance, leading to apoptosis and elastolysis. In anin vitro study of rat lung alveolar L2 cells, cigarette smoke extract (CSE) induced apoptotic cell death. Expo-sure of L2 cells to CSE at a concentration of 0.25% resulted in a 50% increase of caspase-3 and matrix met-alloproteinase (MMP) activities.

11p dell39 03-04-2013 52 3   Download

Analysis of the molecular properties of proteins extracted from organisms living under extreme conditions often highlights peculiar features. We investigated by UV-visible spectroscopy and X-ray crystallography the oxidation pro-cess, promoted by air or ferricyanide, of five hemoglobins extracted from Antarctic fishes (Notothenioidei).

9p dell39 03-04-2013 30 4   Download

The crystal structure of a Drosophila angiotensin-converting enzyme (ANCE) has recently been solved, revealing features important for the binding of ACE inhibitors and allowing molecular comparisons with the structure of human testicular angiotensin-converting enzyme (tACE). ACER is a secondDrosophilaACE that displays both common and dis-tinctive properties.

12p dell39 27-03-2013 39 3   Download

Acidic extracellular pH is a common feature of tumor tissues. We have reported that culturing cells at acidic pH (5.4–6.5) induced matrix metallo-proteinase-9 expression through phospholipase D, extracellular signal regu-lated kinase 1⁄2 and p38 mitogen-activated protein kinases and nuclear factor-jB.

13p galaxyss3 19-03-2013 33 5   Download

Deficiency of the small mitochondrial protein frataxin causes Friedreich’s ataxia, a severe neurodegenerative pathology. Frataxin, which has been highly conserved throughout evolution, is thought to be involved in, among other processes, Fe–S cluster formation. Independent evidence shows that it binds iron directly, although with very distinct features and low affinity.

12p media19 04-03-2013 42 4   Download

Cholesterol oxidases are bifunctional flavoenzymes that catalyze the oxida-tion of steroid substrates which have a hydroxyl group at the 3bposition of the steroid ring system. The enzyme is found, in a wide range of bacte-rial species, in two forms: one with the FAD cofactor bound noncovalently to the enzyme; and one with the cofactor linked covalently to the protein.

18p viettel02 20-02-2013 25 3   Download

Cupins constitute a large and widespread superfamily ofb-barrel proteins in which a mononuclear metal site is both a conserved feature of the struc-ture and a source of functional diversity. Metal-binding residues are con-tributed from two core motifs that provide the signature for the superfamily.

15p viettel02 20-02-2013 27 4   Download

Hepatopathy and hepatomegaly as consequences of prolonged fasting or illnesses are typical clinical features of very long chain acyl-CoA dehydro-genase (VLCACD) deficiency, the most common long-chain fatty acid b-oxidationdefect. Supplementationwithmedium-chain triglycerides (MCTs) is an important treatment measure in these defects, in order to supply suffi-cient energy.

10p viettel02 19-02-2013 34 3   Download