Molecular genetic manipulations

Ebook "Biotechnology of crucifers" despite the recent advances made in the improvement of crucifer crops using conventional breeding techniques, the yield levels and the oil and meal quality could not be improved as expected. The understanding of genetic material (DNA/RNA) and its manipulation by scientists has provided the opportunity to improve crucifers by increasing its diversity beyond conventional genetic limitations.

249p lucchinguyen 28-12-2023 4 2   Download

The Drosophila central nervous system (CNS) is a convenient model system for the study of the molecular mechanisms of conserved neurobiological processes. The manipulation of gene activity in specific cell types and subtypes of the Drosophila CNS is frequently achieved by employing the binary Gal4/UAS system.

11p vihagrid 30-01-2023 4 3   Download

Trichophyton rubrum is an obligate human parasitic fungus and responsible for approximately 80–90% of dermatomycosis in human. Molecular genetic manipulations of this pathogen are challenging and available tools and protocols are only rudimentary.

9p vihamax2711 21-04-2020 12 2   Download

Transformation is the process by which the genetic make-up of an organism is altered by the insertion of new gene into its genome. As most of the agronomic traits are polygenic in nature, so the integration of multiple genes is required to manipulate the complex, polygenic metabolic or regulatory pathways, while ensuring their stable inheritance and expression in succeeding generations. There are various methods for multiple gene transformation but co-transformation method has proved more efficient and transplastomic technology can be used whenever have an option.

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Tomato (Solanum lycopersicum L.) is one of the most important vegetable crops under solanaceous group which can be grown both under open field conditions and greenhouses. Tomato has achieved high popularity especially in the recent years because of lycopene’s anti-oxidative activities and anti-cancerous functions. The cultivated tomato has been used in genetic studies because of the ease with which it can be easily manipulated and also its diversity present within the population.

9p kequaidan2 13-12-2019 15 0   Download

The document highlights the signif- icance of the molecular approaches for all biological processes in both simple and complex cells. The text also incorporates the most recent refer- ences and has been written for students as well as for teachers of molecular biology, molecular genetics, or biochemistry. Invite you to refer.

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This paper discusses how genetic and genomic tools may be used to understand the genetical and molecular control of cereal quality and to develop tools for its manipulation by conventional and genetic engineering approaches.

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In this chapter, you will learn to understand the importance of plasmids and viruses to genetic engineering. Know the natural function of restriction endonucleases and how a normal bacterial cell protects its DNA from their activity. Understand how “sticky ends” are formed and their importance to gene technology. Describe how a chimeric genome is constructed,...

57p tangtuy07 02-04-2016 38 2   Download

Polymerase Chain Reaction is widely held as one of the most important inventions of the 20th century in molecular biology. Small amounts of the genetic material can now be amplified to be able to a identify, manipulate DNA, detect infectious organisms, including the viruses that cause AIDS, hepatitis, tuberculosis, detect genetic variations, including mutations, in human genes and numerous other tasks.

62p zingzing09 24-04-2013 49 5   Download

Research in diatom biology has entered the postgenomic era since the recent completion of the Thalassiosira pseudonanagenome project. How-ever, the molecular tools available for genetic manipulation of diatoms are still sparse, impeding the functional analysis of diatom genes in vivo. Here we describe the first method for inducible gene expression in transgenic diatoms.

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This diverse collection of research articles is united by the enormous power of modern molecular genetics. Every author accomplished two objectives: (1) making the field and the research described accessible to a large audience and (2) explaining fully the genetic tools and approaches that were used in the research. One fact stands out - the importance of a genetic approach to addressing a problem. I encourage you to read several chapters.

462p phoebe75 19-02-2013 42 9   Download

In particular, we hypothesize that both raising capital in public markets rather than from private sources and the institutional environment in which a firm operates have a systematic influence on its incentives to report earnings that reflect economic performance. Both factors shape the way in which information asymmetries between the firm and its key financing parties are resolved and the role earnings play in the process, which in turn affects the properties of reported earnings (see also Ball et al., 2000).

38p bin_pham 06-02-2013 51 5   Download

Flowering plants now dominate the terrestrial ecosystems of the planet, and there are good reasons for supposing that the flower itself has been a major contributing factor to the spread of the Angiosperms. Although the Angiosperm flower is based on four whorls of organs – petals, sepal, stamens and carpels – the 300 000 odd species exhibit a huge array of different floral morphologies. Consider, e.g., the humble periwinkle flower and contrast it with the complex passion flower.

326p bachduong1311 10-12-2012 55 4   Download

One prominent set of patterns from the cross-section has to do with medium-term momentum and post-earnings drift in returns. These describe the tendency for stocks that have had unusually high past returns or good earnings news to continue to deliver relatively strong returns over the subsequent six to twelve months (and vice-versa for stocks with low past returns or bad earnings news). Early work in this area includes Jegadeesh and Titman (1993) on momentum and Bernard and Thomas (1989, 1990) on post-earnings drift.

49p bocapchetnguoi 05-12-2012 65 3   Download

Controlling the impact of stress on brewing biomass, predicting yeast activity and ensuring consistent fermentation performance through successive fermentations remain areas of active interest for the brewing industry. To be able to control and perhaps even manipulate yeast activity, it is necessary to identify factors that affect its functionality during fermentation. Genetic stability and integrity are crucial to maintaining predictable performance.

321p qdung92ct 27-10-2012 87 21   Download

Due to their usefulness in characterizing and manipulating genetic factors responsible for qualitative as well as quantitative traits,molecularmarkersareconsideredto be valuable tools for crop improvement. Theseusesofmolecularmarkershavebeen invaluable in helping researchers understand complex traits, dissect them into singleMendeliangeneticfactors,andestablishtheirchromosomallocationsviatheuse oflinkagemapsand/orcytogeneticstocks.

47p ngovanquang12c3 17-12-2010 89 8   Download