Monoclonal antibodies

Though the hybridoma technology has been widely applied in the production of monoclonal antibodies, it has existed some disadvantages including low yield and genetic instability. Therefore, an alternative approach should be taken into account. Recently, recombinant monoclonal antibody technology has emerged as the best choice to cure hybridoma related drawbacks.

10p vibego 02-02-2024 3 1   Download

Human epidermal growth factor receptor 2 (HER2) has been identified as a molecular target for breast cancer therapy, such as Trastuzumab (Herceptin®). This has been shown to improve patient survival substantially.

10p vibego 02-02-2024 7 0   Download

In this study, we developed a mAb against CA125 to be used as a capture antibody in ELISA technique for detecting CA125 concentration in sera of OC and BC patients. The results of CA125 level in this study were compared with that performed using a commercial CA125 ELISA kit.

8p nguaconbaynhay10 20-02-2021 9 2   Download

Hepcidin (H25) is a hormone peptide synthesized by the liver that binds to ferroportin and blocks iron export. In this study, H25 was inhibited by administration of single and multiple doses of an anti-H25 monoclonal antibody Ab 12B9m in cynomolgus monkeys. The objective of this analysis was to develop a pharmacodynamic model describing the role of H25 in regulating iron homeostasis and the impact of hepcidin inhibition by Ab 12B9m. Total serum H25 and Ab 12B9m were determined in each animal. Corresponding measurements of serum iron and hemoglobin (Hb) were obtained.

15p caothientrangnguyen 09-05-2020 12 1   Download

Nghiên cứu này hướng đến đánh giá hệ vận chuyển (ET) hướng đích thông qua việc RT đánh dấu được tế bào ung thư đại trực tràng, có hiệu suất cao mà không đánh dấu tế bào lành. Nghiên cứu đã sử dụng hệ thống quang phổ hồng ngoại biến đổi fourier Impact 410 NICOLET để xác định hệ vận chuyển có khả năng phát quang, sử dụng kĩ thuật flow cytometry để xác định số lượng tế bào được đánh dấu.

5p kequaidan3 10-03-2020 39 2   Download

Kháng thể đơn dòng là kháng thể kháng một kháng nguyên nào đó được tạo ra từ một dòng tế bào lymphocyte B trong điều kiện in vitro. Và để hiểu rõ hơn về lĩnh vực này, mời các bạn tham khảo bài giảng Sản xuất kháng thể đơn dòng (Monoclonal antibodies-Mab) sau.

53p maiyeumaiyeu09 14-09-2016 222 25   Download

P-glycoprotein (Pgp), a membrane pump often responsible for the multidrug resistance of cancer cells, undergoes conformational changes in the presence of substrates/modulators, or upon ATP depletion, reflected by its enhanced reactivity with the UIC2 monoclonal antibody. When the UIC2-shift was elicited by certain modulators (e.g. cyclosporin A or vinblastine, but not with verapamil or Tween 80), the subsequent binding of other monoclonal anti-Pgp Ig sharing epitopes with UIC2 (e.g. MM12.10) was abolished [Nagy, H., Goda, K., Arceci, R., Cianfriglia, M., ´ Mechetner, E. & Szabo Jr, G.

6p system191 01-06-2013 62 7   Download

Affinity reagents capable of selective recognition of the different human immunoglobulin isotypes are important detection and purification tools in biotechnology. Here we describe the development and characterization of affinity proteins (affibodies) showing selective binding to human IgA. From protein libraries constructed by combinatorial mutagenesis of a 58-amino-acid, three-helix bundle domain derived from the IgG-binding staphylococcal protein A, variants showing IgA binding were selected by using phage display technology and IgA monoclonal antibodies (myeloma) as target molecules. ...

9p system191 01-06-2013 39 3   Download

Mucin glycoproteins on breast cancer cells carry shortened carbohydrate chains. These partially deglycosylatedmucin 1 (MUC-1) structures are recognized by the monoclonal antibody SM3, which is being tested for its diagnostic utility. We used NMR spectroscopy to analyze the binding mode and thebindingepitopeof peptideandglycopeptideantigens to the SM3 antibody. The pentapeptide PDTRP and the glycopentapeptide PDT(O-a-D-GalNAc)RP are known lig-ands of the monoclonal antibody.

12p research12 29-04-2013 40 3   Download

Hybridomas are cells that have been engineered to produce a desired antibody in large amounts. To produce monoclonal antibodies, Bcells are removed from the spleen of an animal that has been challenged with the relevant antigen. These B-cells are then fused with myeloma tumor cells that can grow indefinitely in culture (myeloma is a B-cell cancer). This fusion is performed by making the cell membranes more permeable. The fused hybrid cells (called hybridomas), being cancer cells, will multiply rapidly and indefinitely and will produce large amounts of the desired antibodies.

7p zingzing09 24-04-2013 70 4   Download

ThemaltoseATP-binding cassette transporter ofSalmonella typhimurium is composed of a membrane-associated com-plex (MalFGK2) and a periplasmic receptor (MalE). In addition to its role in transport, the complex acts as a repressor ofmaltose-regulatedgene expressionand is subject to inhibition in the process of inducer exclusion. These activities are thought to be mediated by interactions of the ATPase subunit, MalK, with the transcriptional activator, MalT, and nonphosphorylated enzyme IIA of the glucose phosphotransferase system, respectively. ...

12p research12 23-04-2013 35 3   Download

Previously, a phase-variable epitope was detected in the virulent wild-type strain RC1 of Legionella pneumophila serogroup 1 subgroup OLDA using a lipopolysaccharide-speci®c monoclonal antibody, mAb 2625 [LuÈneberg, E., ZaÈhringer,U.,Knirel,Y.A., Steinmann,D.,Hartmann,M., Steinmetz, I., Rohde, M., Kohl, J. & Frosch, M. (1998) J. Exp. Med.188, 49±60]. In the present study, an isogenic mutant strain, termed 5215, was constructed by deletion of genes involved in the biosynthesis of the mAb 2625 epitope....

13p research12 23-04-2013 29 3   Download

An arti®cial peroxidase-like hemoprotein has been obtained by associating a monoclonal antibody, 13G10, and its iron(III)±a,a,a,b-meso-tetrakis(ortho-carboxyphenyl)por-phyrin [Fe(ToCPP)] hapten. In this antibody, about two-thirds of the porphyrinmoiety is inserted in the binding site, its ortho-COOH substituents being recognized by amino-acids of the protein, and a carboxylic acid side chain of the protein acts as a general acid base catalyst in the heterolytic cleavage of the O±O bond of H2O2, but no amino-acid res-idue is actingas anaxial ligandof the iron....

11p research12 23-04-2013 35 2   Download

Phosphorylation of RNA polymerase II’s largest subunit C-terminal domain (CTD) is a key event during mRNA metabolism. Numerous enzymes, including cell cycle-dependent kinases and TFIIF-dependent phosphatases tar-get the CTD. However, the repetitive nature of the CTD prevents determination of phosphorylated sites by conven-tional biochemistry methods. Fortunately, a panel of monoclonal antibodies is available that distinguishes between phosphorylated isoforms of RNA polymerase II’s (RNAP II) largest subunit....

12p tumor12 20-04-2013 49 2   Download

A high-affinity monoclonal antibody (M27), raised against the human thrombin–antithrombin complex, has been identifiedand characterized. The epitope recognizedbyM27 was located to the linear sequence FIREVP (residues 411– 416), located in the C-terminal cleavage peptide of anti-thrombin.This regionoverlaps, by two residues, theputative binding site of antithrombin for the serpin–enzyme complex receptor.

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Neutralizing and protective monoclonal antibodies (mAbs) were used to fine-map the highlyconserved hemagglutinin noose epitope (H379–410, HNE) of themeasles virus. Short peptides mimicking this epitope were previouslyshown to induce virus-neutralizing antibodies [El Kasmi et al. (2000) J. Gen. Virol.81, 729–735]. The epitope contains three cys-teine residues, two of which (Cys386 and Cys394) form a disulfide bridge critical for antibodybinding.

13p tumor12 20-04-2013 36 5   Download

Therehas longbeenconjectureover thedegree towhichthere may be structural and functional heterogeneity in the tetra-mericserineproteasetryptase(EC 3.4.21.59), amajor mediator of allergic inflammation. We have applied 2D gel electrophoresis to analyze the extent, nature, and variability of thisheterogeneity inlysatesofmast cells isolatedfromlung and skin, and in preparations of purified tryptase. Gels were silver stained, or the proteins transferred to nitrocellulose blots and probed with either tryptase-specific monoclonal antibodies or various lectins. ...

14p tumor12 20-04-2013 39 3   Download

Complement regulator C4b-binding protein (C4BP) and the anticoagulant vitamin K-dependent protein S form a high affinity complexin human plasma. C4BP is composed of sevena-chains and a uniqueb-chain, each chain comprising repeating complement control protein (CCP) modules. The binding site for protein S mainly involves the first of the threeb-chain CCPs (CCP1). However, recently it has been suggested that CCP2 of theb-chain also contributes to the binding of proteinS.

8p tumor12 20-04-2013 33 2   Download

Strains ofPseudomonas syringaepv. porri are characterized by a number of pathovar-specific phenotypic and genomic characters and constitute a highly homogeneous group. Using monoclonal antibodies, they all were classified in a novelP. syringaeserogroup O9. The O polysaccharides (OPS) isolated from the lipopolysaccharides (LPS) of P. syringaepv.

8p tumor12 20-04-2013 38 3   Download

Plasminogen activator inhibitor-1 (PAI-1) belongs to the serpin family of serine proteinase inhibitors. Serpins inhibit their target proteinases by an ester bond being formed between the active site serine of the proteinase and the P1 residue of the reactive centre loop (RCL) of the serpin, fol-lowed by insertion of the RCL intob-sheet A of the serpin. Concomitantly, there are conformational changes in the flexible joint region lateral tob-sheet A.

8p fptmusic 16-04-2013 28 2   Download