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Multiple conserved motifs
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The cDNA encoding lipase enzyme was cloned from Olea europaea var. europaea cv. Gemlik leaves using the RACE method. The full nucleotide sequence was 1546 bp long and encoded a putative protein of 412 amino acid long protein. The amino acid sequence showed identity to Triacylglycerol Lipase 1 sequences from different plants. A phylogenetic tree was constructed showing relations with various plant lipases.
8p
tudichquannguyet
29-11-2021
11
1
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Expansins (EXPs), a group of proteins that loosen plant cell walls and cellulosic materials, are involved in regulating cell growth and diverse developmental processes in plants. However, the biological functions of this gene family in cotton are still unknown.
13p
vijichea2711
28-05-2021
19
1
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Limitedproteolysis of the pyruvate decarboxylase (E1,a2b2 ) component of the pyruvate dehydrogenase (PDH) multi-enzyme complexofBacillus stearothermophilushas indicated the importance for catalysis of a site (Tyr281-Arg282) in the E1asubunit (Chauhan, H.J., Domingo, G.J., Jung, H.-I. & Perham, R.N. (2000)Eur. J. Biochem.267, 7158–7169). This site appears to be conserved in the a-subunit of hetero-tetrameric E1s and multiple sequence alignments suggest that there are additional conserved amino-acid residues in this region, part of a common pattern with the consensus sequence -YR-H-D-YR-DE-.
10p
tumor12
20-04-2013
52
2
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We describe a strategy for systematic amplification of chitin synthase genes (chs) in the filamentous ascomycetes plant-pathogenBotrytis cinereausing PCR with multiple degen-erate primers designed on specific and conserved sequence motifs. Eight distinctchsgeneswere isolated, namedBcchs I, II, IIIa, IIIb, IV, V, VIandVII. They probably constitute the entire chsmultigenic family of this fungus, as revealed by careful analysis of six euascomycetes genomes.
12p
dell39
03-04-2013
35
3
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A homologue of interleukin 18 has been identified from rainbow trout,Oncorhynchus mykiss. The trout IL-18gene spans 3.7 kb and consists of six exons and five introns, sharing the same gene organization with its human coun-terpart. The putative translated protein is 199 amino acids in length with no predicted signal peptide. Analysis of the multiple sequence alignment reveals a conserved ICE cut site, resulting in a mature peptide of 162 amino acids. The trout IL-18 shares 41–45% similarity with known IL-18 molecules and contains an IL-1 family signature motif....
11p
dell39
03-04-2013
51
2
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The problem breaks down into two parts: defining the clusters (i.e., a list of members for each family) and building multiple alignments of the members. Previous approaches to construct comprehensive fam- ily databases have either concentrated on aligning short conserved regions, 6–8 often starting from the manually constructed clusters in Prosite, 9 or full domain alignments using either clusters that were derived manually from PIR2 or automatically. 10 An issue here is whether to aim for conserved regions only or whole domain alignments.
9p
yasuyidol
02-04-2013
39
3
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