Multiple shoot regeneration

The procedure reported in this article is useful for regeneration of drumstick plantlets from shoots which is a mean of propagation of plants with the identical genetic traits for field studies.

10p vilarry 01-04-2024 1 1   Download

This study reported on micro propagation protocol for in-vitro propagation of B. balcooa through axillary shoot. This is useful for land reclamation through bamboo plantation and help for rural economy and livelihood promotion.

5p cothumenhmong9 04-01-2021 7 0   Download

The present investigation was emphasized on development of an efficient in vitro micropropagation protocol for high frequency mass multiplication of true-to-type plants and to detect the genetic uniformity of micropropagated plants of Stevia using molecular markers.

13p nguathienthan8 20-10-2020 12 2   Download

In addition, the effects of explant age and lighting condition on shoot regeneration were also investigated. After 8 weeks of culture, the results showed that shoot regeneration rate (100%) and shoot multiplication coefficient (13.33) of the in vitro leaf-tTCL-4 were higher than those of other treatments and control. The shoot regeneration rate of P. edulis Sims. also varied with the change of explant age. The highest shoot regeneration rate (100%) was obtained from leaf explants of 1.5-month-old shoots after 8 weeks of culture.

9p nguyenxuankha_bevandan 14-08-2020 16 2   Download

Momordica cymbalaria Fenzl. is a perennial herbaceous climber belongs to cucurbitaceae family and the genus of bitter gourd (Momordica charantia). Fruits are used as vegetables, it possess hypoglycaemic, hypolipidemic, cardio protective, hepatoprotective, nephroprotective and antioxidant properties. It is rich in crude fiber (6.42 g/100 g), calcium (72 g/100g), potassium, sodium and vitamin C (290 g/100g).The major constraints in propagation of Momordica cymbalaria are non-availability of standardized propagation methods and low yield.

11p angicungduoc6 20-07-2020 20 1   Download

An efficient protocol was developed for commercial multiplication of gerbera using young capitulum as explant across different varieties of gerbera. Media for culture establishment, shoot regeneration, multiplication were standardized. Mercuric chloride treatment (0.1%) for 2 minutes was found to be the best surface sterilant for capitulum explants resulting in the minimum per cent contaminated growing cultures (17.27%).

14p gaocaolon5 14-06-2020 11 2   Download

In vitro regeneration and in planta transformation protocols were standardized for the black gram (Vigna mungo L.) cv. VBN 3, an important grain legume. An efficient regeneration protocol was developed using 1-week-old de-embryonated cotyledon as explants. The maximum frequency of shoot regeneration (72.6%), multiple shoot induction (6.0 ± 0.3) and shoot elongation was achieved on MS medium supplemented with 3.0 mg/l 6-benzyl amino purine (BAP) after 4 weeks of culture. Up to 65% of the regenerated shoots were rooted on MS medium containing 0.

15p angicungduoc4 26-04-2020 17 0   Download

Shoot apices of Physalis minima L. were cultured on fifteen full strength MS medium supplemented with various concentrations of cytokinins (BAP, KIN and TDZ). Tissue culture responses namely elongation of existing shoot and differentiation of multiple shoots were observed from the cultured explants. The establishment of cultured explants was not affected by the cytokinin concentration in the medium but overall shoot apices showed better establishment on medium supplemented with BAP than other cytokinins.

7p nguathienthan4 21-04-2020 20 1   Download

An effective protocol for in vitro proliferation and multiplication of Gerbera cv. Partrizia was established during 2015-17. Among the two stages initially used for culture initiation, capitulum explants cultured at immature stage responded better than the mature stage. Best establishment of immature capitulum explants and more number of quality shoots was obtained on modified MS medium supplemented with 10 mg/l BAP and 1 mg/l IAA. Half strength MS medium containing NAA (0.5 mg/l) + IBA (0.5 mg/l) was best for root induction.

6p nguathienthan4 18-04-2020 14 1   Download

Effective surface sterilization and shoot regeneration protocols for Clinacanthus nutans are described. Nodal segments of C. nutans were sterilised by single- and double-stage sterilisation methods. In the double-stage method, explants were soaked in Plant Preservative Mixture (PPM) at various concentrations (0, 1.25, 2.50, 5.0 µL/mL).

12p trinhthamhodang1212 06-04-2020 10 1   Download

Capsicum annuum L. is an important vegetable crop of India and grown widely in open field as well as polyhouses. Low seed germination and higher price of seeds is major problem associate with the cultivation of this crop. To overcome these problems, plant tissue culture offers technology to generate a nursery in minimum duration with several other benefits. For this purpose, a study was designed to evaluate the effect of benzyl amino purine (BAP) on seed germination and multiplication shoot induction from shoot apex and nodal segment. Explants were obtained from in vitro grown seedlings.

7p cothumenhmong4 25-03-2020 14 1   Download

Wheat is the second most produced staple food crop in world. An efficient method for in vitro micro propagation and genetic transformation of wheat are crucial for both basic and applied research. The present study was initiated with a view to develop a regeneration system in 4 genotypes of wheat cultivars HD2888, HD2329, DBW148, and DBW88 as a prerequisite to transformation. The mature embryos were excised from seeds and cultured on MS medium supplemented with high and low concentrations of cytokinins and auxins respectively.

8p nguaconbaynhay4 22-03-2020 16 2   Download

Regeneration protocol is pre-requisite for the development of transgenic plants. Maize (Zea mays L) crop is severely affected by fungal disease turcicum leaf blight. In the present study, standardizing the regeneration protocol for maize genotype was carried for the development of transgenic maize against turcicum leaf blight. The direct regeneration of maize involved nodal explants from the maize coleoptiles.

11p chauchaungayxua4 18-03-2020 11 1   Download

An in vitro protocol for plant regeneration through organogenesis was established for Picrorhiza kurroa Royle Ex. Benth (Scrophulairaceae). The plant is a principle source of glycoside that is Picroside-I, Picroside-II and Kutkoside. Callus was induced on Murashige and Skoog basal medium supplemented with cytokinin (Kn or BA) and auxin (2,4-D/IBA/NAA) using leaf, petiole and stem explants. Shoots were regenerated on MS media with BA (1.5 to 2.5 mgl-1 ) combined with auxin (IBA or NAA) in the range of 0.5 to 2.5 mgl-1 . Rooting was initiated with full or half media supplemented with 0.

7p cothumenhmong3 22-02-2020 14 1   Download

Gladiolus (Gladiolus hybrida) is an important ornamental plant cultivated world over for its attractive spikes. The commercial cultivation of Gladiolus is based on natural multiplication of corms and cormels. In vitro propagation techniques, is superior to conventional propagation and produces disease free plants in huge quantities. In vitro regeneration of gladiolus cultivars, Sylvia, White Prosperity and Amsterdam was achieved using shoot bud of cormels as an explant. Sylvia variety recorded the best for callus induction and regeneration than other two varieties.

6p trinhthamhodang3 14-02-2020 18 0   Download

Somatic embryogenesis based plant regeneration is a pre-requisite for genetic transformation of banana. Mass multiplication of banana through somatic embryogenesis is also viewed as an economically viable alternative to commonly used shoot tip based method. Banana somatic embryogenesis response was studied in three popular banana cultivars, Grand Naine, Rasthali and Hill banana „Virupakshi‟. Immature male flower bud explant was used for embryogenic callus induction.

8p chauchaungayxua3 07-02-2020 15 2   Download

Coconut has a wide range of applications from culinary to industrial areas. Loss of coconut trees due to the devastating effect of deadly diseases and the natural aging of the plants demand rejuvenation of orchards with elite seedlings. A conventional breeding program is not sufficient to meet the growing need for healthy planting materials. However, in vitro culture studies can pave the way for mass multiplication. In this study, the effect of TDZ on sliced mature embryo explants through direct organogenesis of coconut var. Chowghat Orange Dwarf (COD) was investigated.

8p chauchaungayxua3 07-02-2020 17 1   Download

Banana variety Nanjanagudu rasabale has been found growing in parts of Mysuru district, known for its unique aroma, flavour, taste and shelf life but unfortunately deemed as an endangered variety. Having given GI protection hope for bringing this variety back to field by producing disease free tissue culture plants demanded development of an efficient protocol for in vitro regeneration. In the present investigation, both citric acid and ascorbic acid found effective in inhibiting browning of shoot tip explant due to phenolic compounds.

11p nguaconbaynhay3 07-02-2020 9 0   Download

An efficient reproducible protocol for the in vitro multiplication of cucumber (Cucumis sativus L.), an important vegetable crop, was developed from nodal explants. Addition of casein hydrolysate to the shoot induction medium (MS + BA) significantly enhanced the number of multiple shoots or growth of the regenerants.

4p vineptune2711 31-10-2019 14 0   Download

This study reports, for the first time, an efficient in vitro plant regeneration protocol for Digitalis lamarckii Ivan. Via direct shoot organogenesis. Two sets of experiments were carried out; the first compared different concentrations of 6-benzylaminopurine (BAP), kinetin, thidiazuron (TDZ), and zeatin alone using leaf explants excised from in vitro germinated seedlings, while the second set tested the combinations of indole-3-butyric acid (IBA).

7p vibasque27 27-03-2019 8 0   Download