Multiplex PCR for detection

Part 1 book "Protocols for the diagnosis of pig viral diseases" includes content: Requirements and preparedness for attending a viral disease outbreak in pig farms; collection of samples, their preservation and transportation; methods for quantification of viruses; protocols for isolation of genetic materials from RNA viruses; multiplex pcr for diagnosis of porcine diseases; protocols for isolation of plasmid DNA,... and other contents.

203p muasambanhan02 18-12-2023 6 3   Download

Part 1 book "ELISA - Methods and protocols" includes content: Flow through assay for detection of antibodies using protein a colloidal gold conjugate as a probe; multiplexed microsphere suspension array based immunoassays; lateral flow immunoassay, immuno pcr assay for sensitive detection of proteins in real time; tyramide signal amplification for immunofluorescent enhancement;.... and other contents.

116p oursky05 20-09-2023 3 2   Download

The incidence of hypervirulent epidemic Clostridium difficile has increased around the world and now in Australia. Assays that are capable of rapidly identifying these strains would enable earlier diagnosis and timely infection control response. The aims of the first part of the study were to validate and develop a molecular technique for the rapid diagnosis of toxigenic C.

134p runthenight07 01-03-2023 6 2   Download

African swine fever virus (ASFV), classical swine fever virus (CSFV) and atypical porcine pestivirus (APPV) have caused great economic losses to the swine industry in China. Since coinfections of ASFV, CSFV and APPV occur in certain pig herds, it is necessary to accurately and differentially detect these pathogens in field-collected samples.

13p vidarwin 22-02-2022 11 1   Download

Sepsis and other infectious complications are major causes of mortality and morbidity in patients after cardiac surgery. Whereas conventional blood culture (BC) suffers from low sensitivity as well as a reporting delay of approximately 48–72 h, real-time multiplex polymerase chain reaction (PCR) based technologies like “SeptiFast” (SF) might offer a fast and reliable alternative for detection of bloodstream infections (BSI).

11p vizhangzhidong 29-12-2021 9 0   Download

The third multiplex PCR reaction includes the 5 extended STS sY153, sY160, sY82, sY143, and sY83 and the 2 control sequences SRY and ZFX/Y. Six basic primer sequences and eleven extended primer sequences are redesigned to simultaneously pair and amplify STS in the same multiplex reaction: set of 8 primers for 6 basic STS: 6 basic STS + 2 (SRY, ZFX/Y), 8 extension primers set E1: 6 extended STS + 2 (SRY, ZFX/Y), and 7 extension primers set E2: 5 extended STS + 2 (SRY, ZFX/Y).

8p princessmononoke 29-11-2021 15 1   Download

This study was conducted to detect Ascaris suum, Toxocara canis, and Toxocara cati in naturally infected chicken livers, and then to provide information related to ascarid infections in humans. Ninetyfour chicken liver samples collected at a fresh market were used for this study. DNA was extracted from each minced liver sample (500 mg) by the alkaline lysis method using NaOH 50mM and Tris-HCl (pH 8.0).

6p chauchaungayxua12 09-07-2021 12 2   Download

The methodology used was quite systematic, rapid, reliable, and appropriate for qualitative detection and can be extended for multiplex development in other plant species having SSR marker technology for efficient plant genetic resources management including genetic diversity analysis, cultivar identification and evolutionary studies in plants.

9p chauchaungayxua10 19-03-2021 10 1   Download

Culturing of bronchoalveolar lavage (BAL) fluid is a commonly used method for pathogen detection in pneumonia. However, the sensitivity is low, especially in patients pre-treated with anti-infective agents. The early detection of a pathogen is crucial for the outcome of respiratory tract infections.

8p vimaine2711 26-03-2021 9 2   Download

Multiplex polymerase chain reaction (PCR) is a common enrichment technique for targeted massive parallel sequencing (MPS) protocols. MPS is widely used in biomedical research and clinical diagnostics as the fast and accurate tool for the detection of short genetic variations.

12p vioklahoma2711 19-11-2020 14 2   Download

The present study was designed with the objective to evaluate the prevalence of genes encoding antibiotic resistance for commonly used antibiotics in poultry production in S. aureus isolated from Chicken meat marketed in Chennai City.

5p dayhoctainha 10-09-2020 12 0   Download

The study was carried out in an urban tertiary care centre. Samples were collected from patients of acute wards and relevant clinical history was collected. Imipenem resistance detection and antibiotic susceptibility was done. A multiplex PCR was done on imipenem resistant isolates for detection of resistant genes.

10p cothumenhmong7 08-09-2020 10 2   Download

Interrogate the impact of IKZF1 deletion on therapy-outcomes of adults with common B-cell acute lymphoblastic leukemia. Methods: One hundred sixty-five consecutive adults with common B-cell ALL were tested for IKZF1 deletion and for BCR/ABL. Deletions in IKZF1 were detected using multiplex RQ-PCR, multiplex fluorescent PCR, sequence analysis and multiplex ligation-dependent probe amplification (MLPA).

8p vinaypyidaw2711 26-08-2020 11 1   Download

Food authenticity is currently an issue of major concern for food authorities, since incorrect labelling of animal foods may have remarkable negative consequences. To circumvent this problem, DNA based method had been utilised. The present study was carried out for the detection of meat species by using variability in ATPase Subunit 6 and 8 genes by multiplex PCR.

7p nguaconbaynhay7 15-08-2020 14 2   Download

Gene fusion events resulting from chromosomal rearrangements play an important role in initiation of lung adenocarcinoma. The recent association of four oncogenic driver genes, ALK, ROS1, RET, and NTRK1, as lung tumor predictive biomarkers has increased the need for development of up-to-date technologies for detection of these biomarkers in limited amounts of material.

8p vimanila2711 29-06-2020 5 0   Download

With the introduction of Olaparib treatment for BRCA-deficient recurrent ovarian cancer, testing for somatic and/or germline mutations in BRCA1/2 genes in tumor tissues became essential for treatment decisions.

15p vikuala271 13-06-2020 13 1   Download

The objective of the study was to see the prevalence of C. perfringens as well as to detect the enterotoxigenic genes in C. perfringens strains isolated from different meat and water samples in Kashmir, India. For this 275 different meat and meat product, and water samples were analyzed by standard bacteriological technique. All the Fifty nine (59) isolates recovered from these samples were typed according to their gene by multiplex PCR assay. Out of these, 44 (74.57%) isolates were confirmed as C. perfringens type A based on the presence of alpha toxin gene (cpa).

10p cothumenhmong5 17-05-2020 6 0   Download

A brief study was carried on forty Staphylococcus aureus isolates from Bovine milk from Ludhiana, Punjab, India for detecting the antibiotic resistance profile using E-strips and genotypically targeting various antibiotic resistance genes. The antibiotics used for phenotypic resistance detection were Penicillin, Gentamicin, Erythromycin, Clindamycin, Tetracycline, Chloramphenicol, Ciprofloxacin, Trimethoprim-sulphamethoxazole, Oxacillin, Vancomycin and Amoxyclav.

14p nguaconbaynhay5 16-05-2020 6 0   Download

Most genetically modified (GM) plants contain a promoter, P35S, from the plant virus, Cauliflower mosaic virus (CaMV), and many have a terminator, TNOS, derived from the bacterium, Agrobacterium tumefaciens. Assays designed to detect GM plants often target the P35S and/or TNOS DNA sequences.

12p vihamax2711 21-04-2020 8 1   Download

Amoebiasis, caused by Entamoeba histolytica, remains a serious public health issue worldwide. For decades, microscopy remains preferred method for detection of the pathogenic species. The recent advent of molecular biology has helped in developing appropriate diagnostic technologies, of which isothermal amplification is considered to be robust and cost-effective compared to PCR that requires expensive instruments and highly skilled personnel.

6p quenchua4 06-04-2020 12 2   Download