Native bacillus subtilis

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Bacillus subtilis spore vaccines displaying protective antigen induce functional antibodies and protective potency

Bacillus anthracis is the causative agent of anthrax, a disease of both humans and various animal species, and can be used as a bioterror agent. Effective vaccines are available, but those could benefit from improvements, including increasing the immunity duration, reducing the shot frequency and adverse reactions. In addition, more sophisticated antigen delivery and potentiation systems are urgently required.

10p vigandhi 23-02-2022 8 1 Download

Cloning and expression of maltooligosyltrehalose trehalohydrolase from sulfolobus solfataricus dsm 1616 in bacillus subtilis Wb800

The expressional cassette was integrated into B. subtilis WB800 genome replacing 3’amyE-5’amyE, hindering the native amylase activity of the host. Expression of expected protein was confirmed by electrophoresis SDS-PAGE. From our results, it indicates that gene MTH was expressed successfully in B. subtilis WB800. After 0.5% acetoin induction for 48 h, the data showed that the protein with a molecular mass of ~64 kDa on SDS-PAGE was expressed. The level of recombinant protein in WBpAacoMTH was increased and reached 2.5%, 15.2% and 21.95%, respectively comparing with native B. subtilis WB800.

10p spiritedaway36 25-11-2021 13 0 Download

Efficacy of native bacillus subtilis against postharvest penicillium rot pathogen Penicillium sp. of Khasi Mandarin Oranges in Meghalaya, India

Khasi mandarin orange is an important horticulture crop in Meghalaya but several losses have been reported due to the post-harvest disease Penicillium rot caused by Penicillium sp. B. subtilis was evaluated for its efficacy against Penicillium sp. under in-vitro. A total of 260 isolates were isolated from samples collected from different habitats of Meghalaya. Twelve B. subtilis isolates were identified by morphological, biochemical and molecular identification and studied for their antagonistic properties against Penicillium sp.

14p quenchua6 15-06-2020 12 2 Download
Evaluation of native bacterial consortium from crude oil-impacted tropical environment for integration into bioremediation process

Petroleum-based products are the major source of energy for industry and daily life. Leaks and accidental spills occur regularly during the exploration, production, refining, transport, and storage of petroleum and petroleum products. Crude oil-impacted tropical soil and natural water samples (0 - 30cm depth) were obtained from the Niger Delta, Nigeria. A total of twenty seven bacterial species of relevance in bioremediation were isolated and characterized using standard and conventional methods. The predominant species belong to the genera Pseudomonas and Proteus.

12p chauchaungayxua3 07-02-2020 14 1 Download