Next-generation sequencing data
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Sequencing errors are key confounding factors for detecting low-frequency genetic variants that are important for cancer molecular diagnosis, treatment, and surveillance using deep next-generation sequencing (NGS). However, there is a lack of comprehensive understanding of errors introduced at various steps of a conventional NGS workflow, such as sample handling, library preparation, PCR enrichment, and sequencing. In this study, we use current NGS technology to systematically investigate these questions.
15p vigalileogalilei 27-02-2022 14 2 Download
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The last 10 years have seen the rise of countless functional genomics studies based on NextGeneration Sequencing (NGS). In the vast majority of cases, whatever the species, whatever the experiment, the two first steps of data analysis consist of a quality control of the raw reads followed by a mapping of those reads to a reference genome/transcriptome.
11p visilicon2711 20-08-2021 6 1 Download
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Colossoma macropomum (tambaqui) and Piaractus mesopotamicus (pacu) are good fish species for aquaculture. The tambacu, individuals originating from the induced hybridization of the female tambaqui with the male pacu, present rapid growth and robustness, characteristics which have made the tambacu a good choice for Brazilian fish farms.
17p vilichoo2711 23-06-2021 10 1 Download
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Biological data has grown explosively with the advance of nextgeneration sequencing. However, annotating protein function with wet lab experiments is time-consuming. Fortunately, computational function prediction can help wet labs formulate biological hypotheses and prioritize experiments.
16p vikentucky2711 24-11-2020 12 2 Download
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Cancer immunotherapy has demonstrated significant clinical activity in different cancers. T cells represent a crucial component of the adaptive immune system and are thought to mediate anti-tumoral immunity. Antigenspecific recognition by T cells is via the T cell receptor (TCR) which is unique for each T cell.
14p vioklahoma2711 19-11-2020 12 0 Download
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Chromosomal deletions represent an important class of human genetic variation. Various methods have been developed to mine “next-generation” sequencing (NGS) data to detect deletions and quantify their clonal abundances.
8p viflorida2711 30-10-2020 11 2 Download
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The rapid development of next-generation sequencing (NGS) technology has continuously been refreshing the throughput of sequencing data. However, due to the lack of a smart tool that is both fast and accurate, the analysis task for NGS data, especially those with low-coverage, remains challenging.
14p viconnecticut2711 28-10-2020 13 1 Download
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Next-generation sequencing is revolutionising diagnosis and treatment of rare diseases, however its application to understanding common disease aetiology is limited. Rare disease applications binarily attribute genetic change(s) at a single locus to a specific phenotype.
15p vijisoo2711 27-10-2020 14 1 Download
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Next-generation sequencing technology is developing rapidly and the vast amount of data that is generated needs to be preprocessed for downstream analyses. However, until now, software that can efficiently make all the quality assessments and filtration of raw data is still lacking.
6p vijisoo2711 27-10-2020 13 1 Download
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RNA sequencing (RNA-seq)-based whole transcriptome analysis (WTA) using ever-evolving nextgeneration sequencing technologies has become a primary tool for coding and/or noncoding transcriptome profiling. As WTA requires RNA-seq data for both coding and noncoding RNAs, one key step for obtaining high-quality RNA-seq data is to remove ribosomal RNAs, which can be accomplished by using various commercial kits. Nonetheless, an ideal rRNA removal method should be efficient, user-friendly and cost-effective so it can be adapted for homemade RNA-seq library construction.
12p partimesinhvien 08-05-2020 15 0 Download
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This study presents the first molecularly proven data on natural hybridization in the genus Pulsatilla, including the complete plastome sequence. A plant with morphological characteristics intermediate between P. patens and P. pratensis was found near the village of Bocheniec (southern Poland).
9p caplock2711 21-02-2019 17 1 Download