Next-generation sequencing technologies
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Antibiotic resistance is increasingly popular together with emerging diseases presents an urgent requirement for more and more discovery of novel marine bioactive compounds. Paired-end reads of 16S rRNA sequence of bacteria living in the coral Acropora millepora obtained by the Illumina nextgeneration sequencing technology to be processed by DADA2 pipeline, phyloseq and ggplot2 packages showed that bleached coral mucus had an alpha diversity to be higher than healthy coral mucus. T
6p vimulcahy 18-09-2023 4 3 Download
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Sequencing errors are key confounding factors for detecting low-frequency genetic variants that are important for cancer molecular diagnosis, treatment, and surveillance using deep next-generation sequencing (NGS). However, there is a lack of comprehensive understanding of errors introduced at various steps of a conventional NGS workflow, such as sample handling, library preparation, PCR enrichment, and sequencing. In this study, we use current NGS technology to systematically investigate these questions.
15p vigalileogalilei 27-02-2022 14 2 Download
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Carex L., a grass genus commonly known as sedges, is distributed worldwide and contributes constructively to turf management, forage production, and ecological conservation. The development of nextgeneration sequencing (NGS) technologies has considerably improved our understanding of transcriptome complexity of Carex L. and provided a valuable genetic reference.
15p viansan2711 30-07-2021 9 1 Download
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The rapid development of next-generation sequencing (NGS) technology has continuously been refreshing the throughput of sequencing data. However, due to the lack of a smart tool that is both fast and accurate, the analysis task for NGS data, especially those with low-coverage, remains challenging.
14p viconnecticut2711 28-10-2020 13 1 Download
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The use of whole genome sequence has increased recently with rapid progression of nextgeneration sequencing (NGS) technologies. However, storing raw sequence reads to perform large-scale genome analysis pose hardware challenges.
10p vicoachella2711 27-10-2020 7 0 Download
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Next-generation sequencing technology is developing rapidly and the vast amount of data that is generated needs to be preprocessed for downstream analyses. However, until now, software that can efficiently make all the quality assessments and filtration of raw data is still lacking.
6p vijisoo2711 27-10-2020 13 1 Download
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With the development of sequencing technologies, there may be some disputes on sequencing analysis. The aim of this study was to investigate different allele frequency thresholds of mutations in targeted genes on prognostic analyses using a panel of cancer associated gene exons (CAGE) in oral squamous cell carcinoma (OSCC).
10p vimanila2711 29-06-2020 5 1 Download
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RNA sequencing (RNA-seq)-based whole transcriptome analysis (WTA) using ever-evolving nextgeneration sequencing technologies has become a primary tool for coding and/or noncoding transcriptome profiling. As WTA requires RNA-seq data for both coding and noncoding RNAs, one key step for obtaining high-quality RNA-seq data is to remove ribosomal RNAs, which can be accomplished by using various commercial kits. Nonetheless, an ideal rRNA removal method should be efficient, user-friendly and cost-effective so it can be adapted for homemade RNA-seq library construction.
12p partimesinhvien 08-05-2020 15 0 Download