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Nextgeneration sequencing
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Antibiotic resistance is increasingly popular together with emerging diseases presents an urgent requirement for more and more discovery of novel marine bioactive compounds. Paired-end reads of 16S rRNA sequence of bacteria living in the coral Acropora millepora obtained by the Illumina nextgeneration sequencing technology to be processed by DADA2 pipeline, phyloseq and ggplot2 packages showed that bleached coral mucus had an alpha diversity to be higher than healthy coral mucus. T
6p
vimulcahy
18-09-2023
4
3
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Thiết lập và đánh giá qui trình sàng lọc trước sinh không xâm lấn cho các bệnh đơn gen trội phổ biến
Xét nghiệm sàng lọc trước sinh không xâm lấn NIPT (Non-Invasive Prenatal Testing - NIPT) đang ngày càng được ứng dụng rộng rãi trên thế giới. Gần đây, nhiều nghiên cứu cho thấy NIPT sử dụng kỹ thuật giải trình gen tự thế hệ mới (Nextgeneration sequencing – NGS) có khả năng phát hiện một phổ rộng các bệnh đơn gen dạng di truyền trội. Thiết lập và đánh giá qui trình trước sinh không xâm lấn cho các bệnh di truyền trội đơn gen thông qua việc xác định độ nhạy và độ đặc hiệu kỹ thuật của xét nghiệm.
6p
viginnirometty
28-04-2022
34
1
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Sequencing errors are key confounding factors for detecting low-frequency genetic variants that are important for cancer molecular diagnosis, treatment, and surveillance using deep next-generation sequencing (NGS). However, there is a lack of comprehensive understanding of errors introduced at various steps of a conventional NGS workflow, such as sample handling, library preparation, PCR enrichment, and sequencing. In this study, we use current NGS technology to systematically investigate these questions.
15p
vigalileogalilei
27-02-2022
14
2
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To investigate a possible transmission of blaCMY-2 along the food production chain, we conducted a nextgeneration sequencing (NGS)-based analysis of 164 CMY-2-producing E. coli isolates from humans, livestock animals and foodstuff from Germany.
17p
vitzuyu2711
29-09-2021
10
1
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The last 10 years have seen the rise of countless functional genomics studies based on NextGeneration Sequencing (NGS). In the vast majority of cases, whatever the species, whatever the experiment, the two first steps of data analysis consist of a quality control of the raw reads followed by a mapping of those reads to a reference genome/transcriptome.
11p
visilicon2711
20-08-2021
6
1
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Carex L., a grass genus commonly known as sedges, is distributed worldwide and contributes constructively to turf management, forage production, and ecological conservation. The development of nextgeneration sequencing (NGS) technologies has considerably improved our understanding of transcriptome complexity of Carex L. and provided a valuable genetic reference.
15p
viansan2711
30-07-2021
9
1
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Colossoma macropomum (tambaqui) and Piaractus mesopotamicus (pacu) are good fish species for aquaculture. The tambacu, individuals originating from the induced hybridization of the female tambaqui with the male pacu, present rapid growth and robustness, characteristics which have made the tambacu a good choice for Brazilian fish farms.
17p
vilichoo2711
23-06-2021
10
1
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The molecular characterization information of T-DNA integration is not only required by public risk assessors and regulators, but is also closely related to the expression of exogenous and endogenous genes.
13p
vilichoo2711
23-06-2021
6
1
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Common buckwheat (2n = 2x = 16) is an outcrossing pseudocereal whose seeds contain abundant nutrients and potential antioxidants. As these beneficial compounds are damaged by preharvest sprouting (PHS) and PHS is likely to increase with global warming, it is important to find efficient ways to develop new PHS-tolerant lines.
13p
vijichea2711
28-05-2021
7
1
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Chlamydia psittaci pneumonia is a zoonotic infectious disease caused by Chlamydia psittaci. Diagnostic tools, including culture, serologic test and PCR-based methods, are available but prone to false negative results.
7p
vimaine2711
26-03-2021
11
2
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Biological data has grown explosively with the advance of nextgeneration sequencing. However, annotating protein function with wet lab experiments is time-consuming. Fortunately, computational function prediction can help wet labs formulate biological hypotheses and prioritize experiments.
16p
vikentucky2711
24-11-2020
12
2
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Cancer immunotherapy has demonstrated significant clinical activity in different cancers. T cells represent a crucial component of the adaptive immune system and are thought to mediate anti-tumoral immunity. Antigenspecific recognition by T cells is via the T cell receptor (TCR) which is unique for each T cell.
14p
vioklahoma2711
19-11-2020
12
0
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Targeted resequencing offers a cost-effective alternative to whole-genome and whole-exome sequencing when investigating regions known to be associated with a trait or disease. There are a number of approaches to targeted resequencing, including microfluidic PCR amplification, which may be enhanced by multiplex PCR.
5p
vioklahoma2711
19-11-2020
9
0
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Chromosomal deletions represent an important class of human genetic variation. Various methods have been developed to mine “next-generation” sequencing (NGS) data to detect deletions and quantify their clonal abundances.
8p
viflorida2711
30-10-2020
11
2
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The rapid development of next-generation sequencing (NGS) technology has continuously been refreshing the throughput of sequencing data. However, due to the lack of a smart tool that is both fast and accurate, the analysis task for NGS data, especially those with low-coverage, remains challenging.
14p
viconnecticut2711
28-10-2020
13
1
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The use of whole genome sequence has increased recently with rapid progression of nextgeneration sequencing (NGS) technologies. However, storing raw sequence reads to perform large-scale genome analysis pose hardware challenges.
10p
vicoachella2711
27-10-2020
7
0
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Next-generation sequencing is revolutionising diagnosis and treatment of rare diseases, however its application to understanding common disease aetiology is limited. Rare disease applications binarily attribute genetic change(s) at a single locus to a specific phenotype.
15p
vijisoo2711
27-10-2020
14
1
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Next-generation sequencing technology is developing rapidly and the vast amount of data that is generated needs to be preprocessed for downstream analyses. However, until now, software that can efficiently make all the quality assessments and filtration of raw data is still lacking.
6p
vijisoo2711
27-10-2020
13
1
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Processing and analysis of DNA sequences obtained from nextgeneration sequencing (NGS) face some difficulties in terms of the correct prediction of DNA sequencing outcomes without the implementation of bioinformatics approaches.
13p
vicolorado2711
22-10-2020
13
0
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Endometrioid adenocarcinoma of the uterus and ovarian endometrioid carcinoma share many morphological and molecular features. Differentiation between simultaneous primary carcinomas and ovarian metastases of an endometrial cancer may be very challenging but is essential for prognostic and therapeutic considerations.
9p
vimale2711
21-08-2020
8
1
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