NGS technology

Esophageal cancer (EC) is a deadly disease with limited therapeutic options. Although circulating tumor DNA (ctDNA) could be a promising tool in this regard, the availiable evidence is limited. We performed a systematic review and meta-analysis to summarize the clinical applicability of the next-generation sequencing (NGS) and droplet digital polymerase chain reaction (ddPCR) technology on the ctDNA detection of the EC and listed the current challenges.

12p vikoch 27-06-2024 1 1   Download

Ebook "Advancing development of synthetic gene regulators: With the power of high-throughput sequencing in chemical biology" focuses on an “outside the box” notion by utilizing the powerful applications of next-generation sequencing (NGS) technologies in the interface of chemistry and biology. In personalized medicine, developing small molecules targeting a specific genomic sequence is an attractive goal. N-methylpyrrole (P)–N-methylimidazole (I) polyamides (PIPs) are a class of small molecule that can bind to the DNA minor groove.

123p tracanhphuonghoa1007 22-04-2024 6 2   Download

A comprehensive understanding of cancer has been furthered with technological improvements and decreasing costs of next-generation sequencing (NGS). However, the complexity of interpreting genomic data is hindering the implementation of high-throughput technologies in the clinical context: increasing evidence on gene–drug interactions complicates the task of assigning clinical significance to genomic variants.

15p vibransone 28-03-2024 3 2   Download

Next-generation sequencing technologies have enabled a dramatic expansion of clinical genetic testing both for inherited conditions and diseases such as cancer. Accurate variant calling in NGS data is a critical step upon which virtually all downstream analysis and interpretation processes rely.

13p vibransone 28-03-2024 4 2   Download

Ebook "Advances in the understanding of biological sciences using next generation sequencing (NGS) approaches" provides a global view of the recent advances in the biological sciences and the adaption of the pathogen to the host plants revealed using NGS. Molecular Omic’s is now a major driving force to learn the adaption genetics and a great challenge to the scientific community, which can be resolved through the application of the NGS technologies.

248p manmanthanhla0201 26-02-2024 5 2   Download

Ebook "Brachypodium genomics: Methods and protocols" presents protocols for Brachypodium genomics in numerous areas ranging from marker development, trait evolution, functional genomics, metabolomics, transcriptomics, genomics, and tilling. This book also explores techniques to study the widening genetic base of Brachypodium that will help researchers better understand the model plant using NGS technologies.

309p lucchinguyen 28-12-2023 7 2   Download

The aim of the present study was to determine a common exonic signature to discriminate between different clinical pictures, such as non-metastatic, metastatic and non-disease (healthy), using a sustainable and novel technology in liquid biopsy.

14p vileonardodavinci 23-12-2023 4 2   Download

Part 2 book "Trends and advances in veterinary genetics" includes content: Beyond fifty shades - the genetics of horse colors; major histocompatibility complex associated resistance to infectious diseases - the case of bovine leukemia virus infection; new advances in NGS technologies.

76p oursky09 12-11-2023 5 3   Download

Bài nghiên cứu đề xuất mô hình nhằm xác định các yếu tố ảnh hưởng đến quyết định sử dụng chuỗi cung ứng nông sản trực tuyến tại Việt Nam. Vận dụng mô hình chấp nhận công nghệ TAM2 (The Technology Acceptance Model) và lý thuyết chấp nhận và sử dụng công nghệ UTAUT2 (Unified Theory of Acceptance and Use of Technology), nghiên cứu đề xuất mô hình cho hai đối tượng khách hàng khác nhau: khách hàng chưa sử dụng dịch vụ và khách hàng đã sử dụng dịch vụ mua nông sản trực tuyến.

4p vipierre 02-10-2023 9 4   Download

Nghiên cứu "Một số yếu tố liên quan đến bệnh sâu răng của sinh viên năm thứ ba trường Đại học Y Dược – Đại học Quốc gia Hà Nội năm học 2022-2023" nhằm chỉ ra các yếu tố liên quan đến tình trạng sâu răng của sinh viên năm thứ ba Trường Đại học Y Dược – Đại học Quốc gia Hà Nội. Nghiên cứu mô tả cắt ngang được thực hiện trên 206 sinh viên năm thứ ba Trường Đại học Y Dược, Đại học Quốc gia Hà Nội. Mời các bạn cùng tham khảo!

7p giangnhanly 02-10-2023 4 3   Download

Nghiên cứu sử dụng mô hình chấp nhận công nghệ (Technology Acceptance Model – TAM) trong phân tích ý định sử dụng dịch vụ ngân hàng số (NHS). Mô hình nghiên cứu được xây dựng dựa trên các lý thuyết nền tảng và các kết quả nghiên cứu trước đây đã phân tích và đánh giá tác động của nhận thức tính hữu ích, nhận thức tính dễ sử dụng, thái độ đến ý định sử dụng dịch vụ NHS. Mời các bạn cùng tham khảo bài viết.

10p chieuchieu03 25-04-2023 19 6   Download

Recent advances in the development of sequencing technologies provide researchers with unprecedented possibilities for genetic analyses. In this review, we will discuss the history of genetic studies and the progress driven by next-generation sequencing (NGS), using complex inflammatory bowel diseases as an example.

13p vinarcissa 21-03-2023 8 1   Download

This paper covers a systematic mapping of the academic sustainability transitions literature, compiling existing evidence on the potential risks and adverse effects of using NG. Methane emissions in particular pose large climate risks, while the main barriers to sustainability transitions caused by NG include a crowding-out effect of low-carbon technologies, stranded assets, infrastructure lock-in, and behavioural lock-ins.

18p huangminghao_1902 27-02-2022 18 2   Download

Sequencing errors are key confounding factors for detecting low-frequency genetic variants that are important for cancer molecular diagnosis, treatment, and surveillance using deep next-generation sequencing (NGS). However, there is a lack of comprehensive understanding of errors introduced at various steps of a conventional NGS workflow, such as sample handling, library preparation, PCR enrichment, and sequencing. In this study, we use current NGS technology to systematically investigate these questions.

15p vigalileogalilei 27-02-2022 14 2   Download

Despite rapid progress of next-generation sequencing (NGS) technologies, the disease-causing genes underpinning about half of all Mendelian diseases remain elusive. One main challenge is the high genetic heterogeneity of Mendelian diseases in which similar phenotypes are caused by different genes and each gene only accounts for a small proportion of the patients.

20p vigalileogalilei 27-02-2022 31 1   Download

Identification of acute or recent hepatitis C virus (HCV) infections is important for detecting outbreaks and devising timely public health interventions for interruption of transmission. Epidemiological investigations and chemistry-based laboratory tests are 2 main approaches that are available for identification of acute HCV infection. However, owing to complexity, both approaches are not efficient.

10p vilarryellison 29-10-2021 9 0   Download

Natural rubber is an economically important material. Currently the Pará rubber tree, Hevea brasiliensis is the main commercial source. Little is known about rubber biosynthesis at the molecular level. Next-generation sequencing (NGS) technologies brought draft genomes of three rubber cultivars and a variety of RNA sequencing (RNA-seq) data. However, no current genome or transcriptome databases (DB) are organized by gene.

7p vilarryellison 29-10-2021 10 0   Download

Next generation sequencing (NGS) has become a common technology for clinical genetic tests. The quality of NGS calls varies widely and is influenced by features like reference sequence characteristics, read depth, and mapping accuracy. With recent advances in NGS technology and software tools, the majority of variants called using NGS alone are in fact accurate and reliable. However, a small subset of difficult-to-call variants that still do require orthogonal confirmation exist.

9p vibeauty 23-10-2021 10 1   Download

The advent and ongoing development of next generation sequencing technologies (NGS) has led to a rapid increase in the rate of human genome re-sequencing data, paving the way for personalized genomics and precision medicine. The body of genome resequencing data is progressively increasing underlining the need for accurate and time-effective bioinformatics systems for genotyping - a crucial prerequisite for identification of candidate causal mutations in diagnostic screens.

9p vibeauty 23-10-2021 9 0   Download

Understanding the diversity of repair outcomes after introducing a genomic cut is essential for realizing the therapeutic potential of genomic editing technologies. Targeted PCR amplification combined with Next Generation Sequencing (NGS) or enzymatic digestion, while broadly used in the genome editing field, has critical limitations for detecting and quantifying structural variants such as large deletions (greater than approximately 100 base pairs), inversions, and translocations.

10p vibeauty 23-10-2021 13 0   Download