Nucellus tissue

The protocol for high frequency induction of callus from nucellus tissue was re initiation attained in polyembryonic mango cultivars Vellaikolumban and Olour. Among different sterilization treatments followed, dipping fruits in 70 % ethanol +followed by treating them in Cetrimide 1000 ppm - 15 minutes further treating with NaOCl - 1 % +Tween 20 (one drop per 100 ml) for 15 minutes and Flame sterilisation under laminar air flow found to be the best treatment.

7p caygaocaolon2 11-03-2020 10 1   Download

Nucellar epidermal cells in the micropylar region of Scilla autumnalis L. differentiate into a nucellar cap. It secretes a PAS-positive exudate filling the micropyle. A group of cells at the chalazal end of the nucellus forms a hypostase. Close to the antipodals, the hypostase cells contain abundant starch grains, suggesting a function as a storage tissue.

11p vibasque27 27-03-2019 11 1   Download