Nuclear mitochondrial DNA

Ebook "Mitochondrial DNA: Methods and protocols" is to provide procedures for research scientists and clinicians to analyze mitochondrial DNA and the proteins involved in its maintenance. A review of mitochondrial function, genetics, and diseases is presented in the first chapter by Douglas Wallace. Mitochondrial DNA: Methods and Protocols is organized into two main parts, the first addressing protocols to analyze the DNA.

419p dongmelo 27-05-2024 6 2   Download

Mitochondrial DNA copy number (mtDNA-CN) has been associated with a variety of aging-related diseases, including all-cause mortality. However, the mechanism by which mtDNA-CN influences disease is not currently understood. One such mechanism may be through regulation of nuclear gene expression via the modification of nuclear DNA (nDNA) methylation.

17p vibransone 28-03-2024 2 2   Download

Colorectal adenocarcinomas are characterized by abnormal mitochondrial DNA (mtDNA) copy number and genomic instability, but a molecular interaction between mitochondrial and nuclear genome remains unknown. Here we report the discovery of increased copies of nuclear mtDNA (NUMT) in colorectal adenocarcinomas, which supports link between mtDNA and genomic instability in the nucleus.

15p vioraclene 31-03-2024 5 1   Download

ATPase family AAA-domain containing protein 3A (ATAD3A) is a nuclear-encoded mitochondrial membrane-anchored protein involved in diverse processes including mitochondrial dynamics, mitochondrial DNA organization, and cholesterol metabolism.

21p vibransone 28-03-2024 4 2   Download

To unravel how the integrity of nuclear and mitochondrial circulating cell-free DNA (cfDNA) contributes to its plasma quantity in colorectal cancer (CRC) patients. Methods CfDNA from plasma samples of 80 CRC patients stratified by tumour stage and 50 healthy individuals were extracted. Total cfDNA concentration was determined and equal template concentrations (ETC) were analyzed by quantitative real-time PCR (qPCR) resulting in small and long fragments of KRAS, Alu and MTCO3.

14p vischultz 20-10-2023 4 1   Download

Part 1 book "Advances in animal experimentation and modeling - Understanding life phenomena" includes content: Emerging techniques in biological sciences, molecular basis of animal systematics including barcoding, mitochondrial DNA - A tool for elucidating molecular phylogenetics and population, somatic cell nuclear transfer in cellular medicine and biopharming,.... and other conetnts.

221p oursky06 13-10-2023 6 3   Download

POLG, located on nuclear chromosome 15, encodes the DNA polymerase γ(Pol γ). Pol γ is responsible for the replication and repair of mitochondrial DNA (mtDNA). Pol γ is the only DNA polymerase found in mitochondria for most animal cells. Mutations in POLG are the most common single-gene cause of diseases of mitochondria and have been mapped over the coding region of the POLG ORF.

16p vihagrid 30-01-2023 5 3   Download

There is little knowledge, whether in patients with sepsis neutrophil extracellular trap (NET) formation and NET degrading nuclease activity are altered. Thus, we tested the hypotheses that 1) NET formation from neutrophils of septic patients is increased compared to healthy volunteers, both without stimulation and following incubation with mitochondrial DNA (mtDNA), a damage-associated molecular pattern, or phorbol 12-myristate 13-acetate (PMA; positive control) and 2) that serum nuclease activities are increased as well.

9p vizhangzhidong 29-12-2021 10 0   Download

The genus Sechium includes 11 species, which are highly related to the genus Sicyos. Sechium is native to Mesoamerica, but only S. edule and S. tacaco have been domesticated. Sechium edule is the most exploited species of the genus due to its broad phenotypic variability. A total of 68 samples from the species S. chinantlense, S. compositum, S. hintonii and S. edule were evaluated, as well as their respective varietal complexes (albus, nigrum and virens groups), thereby amplifying the loci of mitochondrial DNA (CoxIII), chloroplast DNA (rbcL) and the nuclear ribosomal ITS1-5.8S-ITS2 region.

13p tudichquannguyet 29-11-2021 11 1   Download

Most genetic analyses of ancient and modern dogs have focused on variation in the autosomes or on the mitochondria. Mitochondrial DNA is more easily obtained from ancient samples than nuclear DNA and mitochondrial analyses have revealed important insights into the evolutionary history of canids. Utilizing a recently published dog Y-chromosome reference, we analyzed Y-chromosome sequence across a diverse collection of canids and determined the Y haplogroup of three ancient European dogs.

9p vibeauty 23-10-2021 9 0   Download

Although high-throughput sequencers (HTS) have largely displaced their Sanger counterparts, the short read lengths and high error rates of most platforms constrain their utility for amplicon sequencing. The present study tests the capacity of single molecule, real-time (SMRT) sequencing implemented on the SEQUEL platform to overcome these limitations, employing 658 bp amplicons of the mitochondrial cytochrome c oxidase I gene as a model system.

14p vibeauty 23-10-2021 15 1   Download

The mitochondrial genomes of higher plants vary remarkably in size, structure and sequence content, as demonstrated by the accumulation and activity of repetitive DNA sequences. Incompatibility between mitochondrial genome and nuclear genome leads to non-functional male reproductive organs and results in cytoplasmic male sterility (CMS). CMS has been used to produce F1 hybrid seeds in a variety of plant species.

15p vitzuyu2711 29-09-2021 12 1   Download

Mitochondrial DNA (mtDNA) copy number has been found associated with multiple diseases, including cancers, diabetes and so on. Both environmental and genetic factors could affect the copy number of mtDNA. However, limited study was available about the relationship between genetic variants and mtDNA copy number. What’s more, most of previous studies considered only environmental or genetic factors. Therefore, it’s necessary to explore the genetic effects on mtDNA copy number with the consideration of PM2.5 exposure and smoking.

9p vitzuyu2711 29-09-2021 6 1   Download

Although horizontal gene transfer (HGT) is a widely accepted force in the evolution of prokaryotic genomes, its role in the evolution of eukaryotic genomes remains hotly debated. Some bdelloid rotifers that are resistant to extreme desiccation and radiation undergo a very high level of HGT, whereas in another desiccationresistant invertebrate, the tardigrade, the pattern does not exist.

12p vijeeni2711 24-07-2021 7 0   Download

Adolescent idiopathic scoliosis (AIS) which characterized by complex three-dimensional deformity of spine has been difficult to cure because of the unknown etiopathology and uncertainty of progression. Nowadays, circulating cell-free (ccf) DNA was found to be a potential biomarker for several benign and malignant diseases.

11p vivermont2711 28-01-2021 13 2   Download

Whole genome and exome sequencing usually include reads containing mitochondrial DNA (mtDNA). Yet, state-of-the-art pipelines and services for human nuclear genome variant calling and annotation do not handle mitochondrial genome data appropriately.

10p viflorida2711 30-10-2020 11 2   Download

Whole-genome sequencing (WGS) projects provide short read nucleotide sequences from nuclear and possibly organelle DNA depending on the source of origin. Mitochondrial DNA is present in animals and fungi, while plants contain DNA from both mitochondria and chloroplasts.

7p viconnecticut2711 29-10-2020 7 2   Download

The nudix family member enzyme MutT homologue-1 (MTH1) hydrolyses the oxidized nucleotides 8-oxo-dGTP and 2-hydroxy-dATP and thus prevents the incorporation of damaged nucleotides into nuclear and mitochondrial DNA.

9p viputrajaya2711 22-06-2020 13 0   Download

We recently reported, using immunoscreening of a T7 complementary DNA (cDNA) library of breast cancer (BC) proteins with sera from patients with BC, the presence of autoantibodies targeting several mitochondrial DNA (mtDNA)-encoded subunits of the electron transport chain (ETC) in complexes I, IV, and V.

12p vikuala271 13-06-2020 11 1   Download

The nuclear DNA is conventionally used to assess the diversity and relatedness among different species, but variations at the DNA genome level has also been used to study the relationship among different organisms. In most species, mitochondrial and chloroplast genomes are inherited maternally; therefore it is anticipated that organelle DNA remains completely associated. Many research studies were conducted simultaneously on organelle genome.

10p viminato2711 22-05-2020 16 1   Download