Nucleic acid modification

Ebook "Modified nucleic acids" spans diverse aspects of modified nucleic acids, from chemical synthesis and spectroscopy to in vivo applications, and highlights studies on chemical modifications of the backbone and nucleobases. Topics discussed include fluorescent pyrimidine and purine analogs, enzymatic approaches to the preparation of modified nucleic acids, emission and electron paramagnetic resonance (EPR) spectroscopy for studying nucleic acid structure and dynamics, non-covalent binding of low- and high-MW ligands to nucleic acids and the design of unnatural base pairs.

280p ladongphongthanh1008 22-04-2024 3 1   Download

Ebook "Mass spectrometry: Developmental approaches to answer biological questions" is structured as follows: after a brief introduction of the usual and most popular applications for mass spectrometry in proteomics, the most recent research and developments in mass spectrometry-based methodologies will be explored. As of now, several investigations tried to improve the quantification of proteins by mass spectrometry, the determination of post-translational modifications, the protein-protein and protein-nucleic acids interaction or the proteins structures.

77p nhanphanguyet 28-01-2024 6 2   Download

Novel, nonperipherally tetrasubstituted cationic metal-free and metallophthalocyanines (Zn, In) were synthesized in the present study. The binding constants, the disappearance of quenching effect of all cationic phthalocyanines on the fluorescence intensity of SYBR Green–disodium salt of deoxyribonucleic acid (DNA) from calf thymus complex, and the changes in Tm of double helix DNA with thermal denaturation profile were investigated by UV-Vis and fluorescence spectrophotometric methods.

22p tudichquannguyet 29-11-2021 8 1   Download

Methods to read out naturally occurring or experimentally introduced nucleic acid modifications are emerging as powerful tools to study dynamic cellular processes. The recovery, quantification and interpretation of such events in high-throughput sequencing datasets demands specialized bioinformatics approaches.

16p vijisoo2711 27-10-2020 4 0   Download

Gene expression and repression in insects is regulated through epigenetic mechanisms i.e. DNA Methylation, Histone modification and Noncoding RNAs which influence the phenotypic modification. In recent days insects are used as models in studying epigenetics. RNA interference (RNAi), also known as RNA silencing, refers to a set of molecular processes in which noncoding RNA molecules target and silence the expression of specific nucleic acids.

11p kethamoi7 26-08-2020 24 1   Download