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PCR based procedures
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In this study, we utilize the genome-wide singlenucleotide polymorphisms (SNPs) data obtained through Multiplexed Inter Simple Sequence Repeat Genotyping by sequencing (MIG-seq) to explore the phylogenetic relationships among Quercus species in Vietnam. The results of this study reveal that all Quercus species in Vietnam belong to subgenus Cerris and the phylogenetic analysis strongly supports the recognition of two infrageneric sections: Quercus and Ilex section for the Vietnamese Quercus.
10p
dianmotminh02
03-05-2024
1
1
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DNA isolation is one of the important steps in conducting PCR-based or real-time PCR-based molecular analysis. This study aims to test the quality of DNA isolation extracted from samples of processed food products from chicken meat (chicken nuggets).
7p
vibego
02-02-2024
0
0
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Part 1 book "PCR Protocols" includes content: Long range PCR with a DNA polymerase fusion, isolation of genomic insertion sites of proviruses using splinkerette PCR based procedures, lariat dependent nested PCR for flanking sequence determination, a global single-Cell cDNA amplification method for quantitative microarray analysis,...and other contents.
171p
oursky06
17-10-2023
5
1
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RNA-seq, a massive parallel-sequencing-based transcriptome profiling method, provides digital data in the form of aligned sequence read counts. The comparative analyses of the data require appropriate statistical methods to estimate the differential expression of transcript variants across different cell/tissue types and disease conditions.
12p
viwyoming2711
16-12-2020
13
0
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The objective of the current study was to identify the effect of reusing the alkali solution on thermoduric count in pasteurized milk. The study was carried out in a commercial Dairy Plant, where reusing of alkali solution is not practiced. In the present study, 28 samples each were collected from raw milk, pasteurized milk, and alkali solution before and after its use. Equivalent numbers of samples from surface swab of storage tank were also collected after cleaning procedure.
9p
chauchaungayxua3
07-02-2020
41
2
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Bovine Leukocyte Adhesion Deficiency (BLAD), Bovine Citrullinaemia (BC), and Complex Vertebral Malformation (CVM) are the common autosomal recessive disorders in cattle affecting dairy industry. The present study was designed to standardize allelespecific PCR based genotyping method for examining the occurrence of these disorders in Indian cattle population. A total of 327 indigenous (Bos indicus), exotic (HF) cattle breeds and HF crossbreds (Bos taurus × Bos indicus) from various parts of India were screened.
9p
nguathienthan2
19-12-2019
31
0
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Bacterial vaginosis (BV) is one of the most common vaginal infections in women of reproductive age. If not treated promptly, the disease can lead to serious complications affecting the fertility and long-term health of women. Research on BV requires effort from a variety of disciplines and its treatment can only be determined by coordinated actions in research and treatment. Currently, BV diagnostic methods are often based on culture techniques and Gram staining. However, molecular biology research is developing and has proved to more advantageous for identifying key pathogens.
5p
caygaocaolon1
13-11-2019
23
0
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In this study, we investigated different methods to establish a rapid diagnostic procedure based on PCR amplification followed by modified SSCP to detect mutation in CEBPA/bZIP. Moreover, we preliminarily screened the mutations in 156 Vietnamese patients.
6p
jangni2
19-04-2018
29
1
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