PCR method

This study aimed to diagnose swine dysentery (SD) caused by Brachyspira hyodysenteriae in pigs by the PCR method in Vietnam. Of the 250 samples, 29 isolates of B. hyodysenteriae (11.60%) were identified by PCR in seven provinces of Northern Vietnam, and the infection rate differed from region to region.

8p vibecca 01-10-2024 3 1   Download

Drug-resistant Tuberculosis (DR-TB) is challenging public health problem in countries with high tuberculosis prevalence and limited resources. Developing and applying the most appropriate and effective methods for diagnosing DR-TB from clinical samples is necessary, allowing a more rapid detection method for large-scale screening.

7p vifaye 20-09-2024 2 1   Download

The rs2681492, a single nucleotide polymorphism in the ATP2B1 gene, has been reported to be associated with cardiovascular diseases and traits including blood pressure, chronic heart failure, and hypertension. The study aimed to develop a PCR-RFLP genotyping method for rs2681492 among the Vietnamese population.

10p viyoko 20-09-2024 6 1   Download

. In this study, 65-bp indel polymorphism in the GOLGB1gene was evaluated in association with body weight in Noi chickens. The live body weight of 170 chickens (90 females, 80 males) at continuous ages of 7-day intervals (from 28 to 84 days) was recorded. A 65-bp indel polymorphism in the GOLGB1 gene was analyzed using the PCR method.

8p dianmotminh02 03-05-2024 4 1   Download

The incidence of hypervirulent epidemic Clostridium difficile has increased around the world and now in Australia. Assays that are capable of rapidly identifying these strains would enable earlier diagnosis and timely infection control response. The aims of the first part of the study were to validate and develop a molecular technique for the rapid diagnosis of toxigenic C.

134p runthenight07 01-03-2023 9 3   Download

To assess the frequency allele and genotypic distribution of polymorphism rs165599 in catechol-O-methyltransferase in Kinh ethnic-patients with schizophrenia. Subjects and methods: A case-control study consisted of 227 schizophrenic patients and 92 healthy controls in the Kinh ethnic. The rs165599 was genotyped by PCR-RFLP and statistical analysis was performed by Chi-Square test. Results: The frequency of allele A and G in schizophrenic patients were 72.36% and 69.94%, respectively. The frequency of allele A and G in healthy controls were 27.64% and 30.06%, respectively (p > 0.05).

6p chauchaungayxua1 02-12-2019 27 3   Download

Cố định vi khuẩn bằng hydroxide kim loại làm tăng độ nhạy của phương pháp PCR khi phát hiện vi khuẩn trong thực phẩm. Vi khuẩn ở nồng độ thấp không qua nuôi dưỡng có thể phát hiện được bằng phương pháp PCR nhờ cô đặc vi khuẩn từ thực phẩm bằng hydroxide kim loại. Enzyme DNase I được sử dụng để loại bỏ DNA từ vi khuẩn sống, do đó phương pháp PCR có thể dùng để phát hiện vi khuẩn sống. SYBR green I được sử dụng trong real-time PCR để định lượng DNA được nhân lên, từ đó xác định số lượng vi khuẩn ban đầu.

9p uocvongxua08 31-08-2015 51 4   Download

Previously, we demonstrated apoptotic cell death in the chorion laeve trophoblast layer of human fetal membrane tissues during the late stages of pregnancy, the progression of apoptosis during incubation in vitro, and its suppression by a low concentration of glucocorticoid hormones.

8p system191 01-06-2013 33 5   Download

Populations in Southeast AsiaandSouthChinahave highfrequencies ofa-thalassemia caused by a-globin gene mutations and/or deletions. This study was designed to find an efficient and simple diagnostic test for the mutations and deletions. A duplex polymerase chain reaction (PCR)/denaturing high-pressure liquid chromatography (DHPLC) was used to detect the mutations and deletions. A blinded study of 110 samples, which included 92 a-thalassemia samples with various genotypes and 18 normal DNA samples, was carried out by the methods.

8p zingzing09 24-04-2013 70 2   Download

The aimof this workwas to elucidate the roles of individual residues within the flexible second binding loop of human cystatin A in the inhibition of cysteine proteases. Four recombinant variants of the inhibitor, each with a single mutation, L73G, P74G, Q76G or N77G, in the most exposed part of this loopwere generated by PCR-based site-directed mutagenesis. The binding of these variants to papain, cathepsin L, and cathepsin B was characterized by equilibrium and kinetic methods.

10p tumor12 22-04-2013 34 2   Download

We used a PCR-based method to determine the genomic DNA sequences encoding phospholipases A2(PLA2s) from the venoms ofVipera aspis aspis(V. a. aspis),Vipera aspis zinnikeri (V. a. zinnikeri), Vipera berus berus(V. b. berus) and a neurotoxicV. a. aspissnake (neurotoxicV. a. aspis) from a population responsible for unusual neurotoxic envenomations in south-east France. We sequenced five groups of genes, each corresponding to a different PLA2.

10p fptmusic 16-04-2013 42 4   Download

Five truncation mutants of chloroplast ATP synthasecsubunit from spin-ach (Spinacia oleracea) lacking 8, 12, 16, 20 or 60 N-terminal amino acids were generated by PCR by a mutagenesis method. The recombinantc genes were overexpressed inEscherichia coliand assembled withabsub-units into a native complex. The wild-type (WT)abcassembly i.e.abcWT exhibited high Mg 2+ -dependent and Ca 2+ -dependent ATP hydrolytic activity.

0p awards 06-04-2013 28 3   Download

A generic sample normalization method applicable in relative comparison of mRNAs quantified with real-time polymerase chain reaction (PCR) is proposed. The method was applied in samples obtained from tomato seeds after osmopriming and aging treatments and from untreated seeds at early imbibition stage, when seeds had not completed germination.

8p dell39 27-03-2013 50 2   Download

Serial analysis of gene expression (SAGE) is a powerful technique for studying gene expression at the genome level. However, short SAGE tags limit the further study of related data. In this study, in order to identify a gene, we developed a semi-nested PCR-based method called the two-step analysis of unknown SAGE tags (TSAT-PCR) to generate longer 3¢-end cDNA fragments from unknown SAGE tags.

7p vinaphone15 28-02-2013 39 2   Download

Serial analysis of gene expression (SAGE) is a powerful technique to study gene expression at the genome level. However, a disadvantage of the short-ness of SAGE tags is that it prevents further study of SAGE library data, thus limiting extensive application of the SAGE method in gene expression studies. However, this problem can be solved by extension of the SAGE tags to 3¢ cDNAs.

12p viettel02 22-02-2013 48 3   Download

Tuyển tập các báo cáo nghiên cứu về hóa học được đăng trên tạp chí sinh học quốc tế đề tài :Validation of a HLA-A2 tetramer flow cytometric method, IFNgamma real time RT-PCR, and IFNgamma ELISPOT for detection of immunologic response to gp100 and MelanA/MART-1 in melanoma patients

25p dauphong3 03-01-2012 51 6   Download

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: A rapid and efficient method for studies of virus interaction at the host cell surface using enteroviruses and real-time PCR

6p dauphong4 24-12-2011 38 3   Download

Abstract Epidermal growth factor receptor (EGFR) kinase domain mutations hyperactivate the kinase and confer kinase addiction of the non-small-cell lung cancer (NSCLC) tumor cells. Almost all of these mutations are located within exons 18-21. The -216 single nucleotide polymorphism in the promoter region is associated with increased EGFR production. We present a method for detecting these common mutations in 81 cases of NSCLC.

6p toshiba23 18-11-2011 68 2   Download

The antiproliferative effect of the Hsp90 inhibitor 17-AAG (17-allylamino-17-demethoxygeldanamycin) on human retinal pigment epithelial cells is investigated. Methods: MTT and flow cytometry were used to study the antiproliferative effects of the 17-AAG treatment of ARPE-19 cells. 2D gel electrophoresis (2-DE) and mass spectrometry were applied to detect the altered expression of proteins, which was verified by real-time PCR.

15p toshiba23 18-11-2011 42 2   Download

The highly pathogenic H5N1 is a major avian pathogen that crosses species barriers and seriously affects humans as well as some mammals. It mutates in an intensified manner and is considered a potential candidate for the possible next pandemic with all the catastrophic consequences. Methods: Nasal swabs were collected from donkeys suffered from respiratory distress. The virus was isolated from the pooled nasal swabs in specific pathogen free embryonated chicken eggs (SPF-ECE).

6p toshiba23 18-11-2011 52 4   Download