Báo cáo khoa học: N-terminal deletion of the c subunit affects the stabilization and activity of chloroplast ATP synthase

Five truncation mutants of chloroplast ATP synthasecsubunit from spin-ach (Spinacia oleracea) lacking 8, 12, 16, 20 or 60 N-terminal amino acids were generated by PCR by a mutagenesis method. The recombinantc genes were overexpressed inEscherichia coliand assembled withabsub-units into a native complex. The wild-type (WT)abcassembly i.e.abcWT exhibited high Mg 2+ -dependent and Ca 2+ -dependent ATP hydrolytic activity.