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PCR system
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Primary central nervous system lymphoma (PCNSL) are rare mature B-cell lymphoproliferative diseases characterized by a high incidence of MYD88 L265P and CD79B Y196 hotspot mutations. The aim of the study was to analyze the detection rate of the MYD88 L265P and CD79B Y196 mutation in cell free DNA (cfDNA) in plasma of patients with PCNSL.
6p
vishanshan
27-06-2024
1
1
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Ebook "How to reliably test for GMOs" have many years of experience in GMO testing, development of real-time PCR methods, implementation of quality system requirements, validations and verification of methods, and measurement uncertainty. The National Institute of Biology is a highly qualified research laboratory and a National Reference Laboratory, which also performs routine analyses of food, feed and seed.
110p
nhanphanguyet
28-01-2024
5
2
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Ebook "Early, rapid and sensitive veterinary molecular diagnostics - Real time PCR applications" gives a comprehensive account of the practical aspects of Real time PCR and its application to veterinary diagnostic laboratories. The optimisation of assays to help diagnose livestock diseases is stressed and exemplified through assembling standard operating procedures from many laboratory sources. Theoretical aspects of PCR are dealt with as well as quality control features necessary to maintain an assured testing system.
319p
tachieuhoa
28-01-2024
6
2
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Ebook "Genome editing in animals: Methods and protocols" details protocols that can be used for generation of knockout animals. Chapters guide the reader through basic protocols for three genome editing technologies, target design tools, and specific protocols for each animal.
258p
lucchinguyen
28-12-2023
4
2
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The modified systemic inflammation score (mSIS) system, which is constructed based on the neutrophil to lymphocyte ratio (NLR) and albumin (Alb), has not been applied to evaluate the prognosis of malignant breast cancer patients who underwent neoadjuvant chemotherapy (NAC). The present study aimed to explore the relationship between the mSIS and overall survival (OS), disease-free survival (DFS) and pathological complete response (pCR).
15p
vileonardodavinci
23-12-2023
8
3
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Part 2 book "Protocols for the diagnosis of pig viral diseases" includes content: Lateral flow assay for diagnosis of pig viral diseases; droplet digital PCR based diagnosis for porcine viral diseases; protocols for immunofluorescence techniques; polymerase spiral reaction (PSR) for the diagnosis of porcine viral diseases; recombinase polymerase amplification based diagnostics of porcine viral diseases; cell culture system for porcine virus isolation and propagation,... and other contents.
180p
muasambanhan02
18-12-2023
5
4
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Pathologic complete response (pCR) following preoperative systemic therapy is associated with improved outcomes after subsequent liver transplant/resection in hepatocellular carcinoma (HCC). However, the relationship between radiographic and histopathological response remains unclear.
12p
visharma
20-10-2023
2
2
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Thyroid cancer is the most frequent malignancy of the endocrine system, of which papillary thyroid cancer (PTC) is the predominant form with a rapid increasing incidence worldwide. Rearranged during transfection (RET) fusions are common genetic drivers of PTC and the potent RET inhibitor selpercatinib has been recently approved for treating advanced or metastatic RET fusion-positive thyroid cancer.
9p
visharma
20-10-2023
8
2
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An elderly 72-year-old man presented with anemia, thrombocytopenia, monocytosis, splenomegaly and lymphadenopathy. Bone marrow biopsy was consistent with mast cell neoplasm with positive CD117, CD25, CD34 myeloblasts and polymerase chain reaction (PCR) revealed mutation of D816V. He developed bilateral femoral neck fractures and biopsy confirmed that he has systemic mastocytosis (SM).
3p
viintuit
26-09-2023
4
0
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Bài viết trình bày xác định tỷ lệ một số đột biến trên gen α-globin và kiểu gen của bệnh HbH bằng kỹ thuật Gap-polymerase chain reaction (Gap-PCR) và kỹ thuật Combine-amplification refractory mutation system- polymerase chain reaction (C-ARMS-PCR).
5p
vioracle
26-09-2023
8
1
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In addition to traditional methods, advanced biotechnologies, especially CRISPR/Cas9-mediated genome editing, have emerged as effective tools for improving important agronomic traits in rice. However, the critical step for utilizing these systems is to develope an effective system for rice transformation and regeneration.
11p
vimulcahy
18-09-2023
4
3
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Infertility is a global concern that affects 15% of couples, and roughly half of those cases are male-specific. Among the genetic factors that contributed heavily to male infertility, TEX15 (testisexpressed gene 15) has been studied across multiple cohorts worldwide and identified to relate to meiotic recombination failure and DNA repair system malfunction.
6p
vimulcahy
18-09-2023
8
3
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Drug-resistant Tuberculosis (DR-TB) is challenging public health problem in countries with high tuberculosis prevalence and limited resources. Developing and applying the most appropriate and effective methods for diagnosing DR-TB from clinical samples is necessary, allowing a more rapid detection method for large-scale screening.
7p
viwolverine
07-07-2023
3
1
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(BQ) Ebook Plant Reverse Genetics: Methods and Protocols – Part 1 presents the following content: Chapter 1 : analysis of high-throughput sequencing data; chapter 2: identification of plant micrornas using expressed sequence tag analysis; chapter 3: microarray data analysis; chapter 4: setting up reverse transcription quantitative-pcr experiments; chapter 5: virus-induced gene silencing in nicotiana benthamiana and other plant species; chapter 6: agroinoculation and agroinfiltration: simple tools for complex gene function analyses; chapter 7: full-length cdna overexpressor gene hunting syst...
134p
runordie7
30-08-2022
7
2
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The efficiency of capecitabine has been proven in early-stage triple negative breast cancer (eTNBC) with residue invasive tumor (non-pCR) after standard neoadjuvant chemotherapy (NACT). However, for those unselected eTNBC patients without screening from NACT (i.e., newly diagnosed eTNBC patients undergoing breast surgery followed by adjuvant systemic therapy), the value of capecitabine has still remains unclear.
11p
viangelamerkel
27-07-2022
4
2
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Ovine footrot caused by Dichelobacter nodosus (D nodosus) is an infectious disease affecting sheep worldwide. Switzerland plans a nationwide footrot eradication program, based on PCR-testing of interdigital swab samples.
8p
vigalileogalilei
28-02-2022
13
1
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Ureaplasma diversum has numerous virulence factors that contribute to pathogenesis in cattle, including Lipid-associated membrane proteins (LAMPs). Therefore, the objectives of this study were to evaluate in silico important characteristics for immunobiological applications and for heterologous expression of 36 LAMPs of U. diversum (UdLAMPs) and, also, to verify by conventional PCR the distribution of these antigens in strains of Brazilian states (Bahia, Minas Gerais, São Paulo, and Mato Grosso do Sul).
16p
vigandhi
23-02-2022
8
1
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The aim of this study was to develop a microfluidic chip to rapidly detect swine pathogenic microorganisms with high-throughput and higher accuracy. Moreover, this chip will decrease the risk of spreading infection during transportation.
8p
vidarwin
23-02-2022
5
1
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The induced resistance was tested using I-ELISA and immunocapture reverse transcription polymerase chain reaction (IC-RT-PCR) for the detection of CMV coat protein gene (cp), which proved that the mentioned symptomless plants were virus-free or with a low level of virus infection.
7p
schindler
30-12-2021
11
0
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The detection of epidermal growth factor receptor (EGFR) mutations in plasma cell-free DNA (cfDNA) is an auxiliary tool for the molecular diagnosis of non-small cell lung cancer (NSCLC), especially when an adequate tumor tissue specimen cannot be obtained.
7p
lazzaro
30-12-2021
12
1
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