Peptide sequence tag
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Accurate structural annotation of genomes is still a challenge, despite the progress made over the past decade. The prediction of gene structure remains difficult, especially for eukaryotic species, and is often erroneous and incomplete.
19p vigiselle2711 30-08-2021 7 1 Download
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The cysteine-rich peptide hepcidin is known to be an antimicrobial peptide and iron transport regulator that has been found in both fish and mam-mals. Recently, we found two different types (designated Hep-JF1 and Hep-JF2) of hepcidin cDNA in the Japanese flounder,Paralichthys oliva-ceus, by expressed sequence tag analysis. The identity of amino acid sequences between Hep-JF1 and Hep-JF2 was 51%. The Hep-JF1 and Hep-JF2 genes both consist of three exons and two introns, and both exist as single copies in the genome. ...
8p fptmusic 12-04-2013 39 1 Download
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Saccharomyces cerevisiaepossesses two glycogenin isoforms (designated as Glg1p and Glg2p) that both contain a con-served tyrosine residue, Tyr232. However, Glg2p possesses an additional tyrosine residue, Tyr230 and therefore two potential autoglucosylation sites. Glucosylation of Glg2p was studied using both matrix-assisted laser desorption ionization and electrospray quadrupole time of flight mass spectrometry. Glg2p, carrying a C-terminal (His6) tag, was produced inEscherichia coliand purified.
12p awards 05-04-2013 35 3 Download
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In the course of systematic identification of peptide signaling molecules combined with the expressed sequence tag database fromHydra, we have identified a novel neuropeptide family that consists of two members with FRamide at the C-terminus; FRamide-1 (IPTGTLIFRamide) and FR-amide-2 (APGSLLFRamide).
11p media19 04-03-2013 32 1 Download