Ph effect on hplc separations

Due to the high toxicity of arsenic and the poison effects to human being, identification and quantification of individual arsenic forms are important to appropriately measure the arsenic toxicity, environmental impact and health risk related to arsenic exposure. Arsenic species were quantified by HPLC-UV-HG-AAS. The separation was performed on an anion exchange Hamilton PRP-X100 (250 mm× 4,1 mm i.d.× 10 µm) column by using mobile phase A: 12 mmol/l KH2PO4- K2HPO4; pH = 6.5 and B: 24 mmol/l KH2PO4-K2HPO4; pH = 6.

6p uocvong02 24-09-2015 78 4   Download

An empirical formula representing the variation of the . quantity with mole fraction of acetonitrile (/) from the values in Table 4-4 could be determined using equation (4-20). The dependence of . versus the mole fraction of ace- tonitrile is shown in Figure 4-25.

30p bigbaby87 03-09-2010 72 13   Download