Phenylalanine hydroxylase activity
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Phenylketonuria (PKU), which is characterized by a deficiency of phenylalanine hydroxylase activity, is an autosomal recessive disorder of phenylalanine (Phe) metabolism. Newborn screening is the main populationbased public health screening program that allows successful identification and treatment of PKU with low-Phe diet.
16p vimanama2711 28-07-2020 10 1 Download
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Cowpea aphid (Aphis craccivora Koch) infestation accumulated phytohormone salicylic acid (SA) in leaves of soybean (Glycine max cv. Namdan) as these soybean plants were at stages R1 (beginning to bloom) and R3 (beginning pod development). Activity of major enzymes involving biosynthesis of SA, such as phenylalanine ammonia-lyase (PAL), benzoic acid 2-hydroxylase (BA2H), was also enhanced under aphid effect.
10p vihermes2711 01-10-2019 7 0 Download
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The catalytic activity of phenylalanine hydroxylase (PAH, phenylalanine 4-monooxygenase EC 1.14.16.1) is regulated by three main mechanisms, i.e. substrate (L-phenylalanine, L-Phe) activation, pterin cofactor inhibition and phos-phorylation of a single serine (Ser16) residue.To address the molecular basis for the inhibition by the natural cofactor (6R)-L-erythro-5,6,7,8-tetrahydrobiopterin, its effects on the recombinant tetrameric human enzyme (wt-hPAH) was studied using three different conformational probes, i.e.
10p tumor12 20-04-2013 32 2 Download
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NMR spectroscopy and X-ray crystallography have provi-ded important insight into structural features of phenyl-alanine hydroxylase (PAH) and tyrosine hydroxylase (TH). Nevertheless, significant problems such as the substrate specificity of PAHand the different susceptibility of TH to feedback inhibition by L-3,4-dihydroxyphenylalanine (L-DOPA) compared with dopamine (DA) remain unre-solved.
11p tumor12 20-04-2013 44 1 Download