Physiological truncation

Oviparously developing embryos of the brine shrimp, Artemia franciscana, synthesize abundant quantities of a small heat shock/a-crystallin protein, termed p26. Wild-type p26 functions as a molecular chaperone in vitro and is thought to help encysted Artemia embryos survive severe physiological stress encountered during diapause and anoxia. Full-length and truncated p26 cDNA derivatives were generated by PCR amplification of p26-3-6-3, then cloned in either pET21(+) or pRSETC and expressed in Escherichia coli BL21(DE3)....

10p research12 01-06-2013 56 5   Download

Uracil in DNA is usually considered to be an error, but it may be used for signaling in Drosophiladevelopment via recognition by a novel uracil-DNA-degrading factor (UDE) [(Bekesi Aet al. (2007) Biochem Biophys Res Commun355, 643–648]. The UDE protein has no detectable similarity to any other uracil-DNA-binding factors, and has no structurally or func-tionally described homologs.

15p mobifone23 18-01-2013 40 3   Download