Phytase activity

Phytase (myo-inositol hexakisphosphate phosphohydrolase)enzyme was isolated from wheat varieties to do kinetic study of phytate degradation. The four wheat varieties selected on the basis of their higher phytase activity for investigation were DBW-17, HD-2894, HUW-234 and LOK-1 wheat variety. Among the four selected wheat varieties, LOK -1 variety reported the maximum phytase activity during enzyme kinetics study.

7p chauchaungayxua12 29-07-2021 10 1   Download

A phytase (myo-inositol-hexakisphosphate phosphohydrolase) enzyme was purified and identified from wheat varieties during process. The four wheat varieties, selected on the basis of their higher phytase activity for investigation were DBW-17, HD-2894, HUW-234 and LOK-1.

8p chauchaungayxua12 29-07-2021 12 1   Download

This review aims to strengthen the state of knowledge of different soil enzymes responsible for transformation of carbon (βglucosidase, xylanase and cellulase), nitrogen (urease and amidohydrolases), phosphorus (phosphatases and phytase) and organic matter decomposition in soil (dehydrogenase, fluorescein diacetate, phenol oxidase and peroxidase).

8p gaocaolon9 22-12-2020 11 1   Download

Probiotics have been extensively studied and explored commercially in many different products in the world. Looking to the demand and health prospects extensive screening for Lactic Acid Bacteria (LAB) with probiotic potentials was investigated. Total 57 bacterial isolates were collected from the 32 samples. Out of which, 33 LAB isolates were confirmed on the basis of morphological and preliminary biochemical characterization. On the basis of probiotic traits such as low pH and high bile salt tolerance, 8 LAB isolates with probiotic potentials were selected.

7p caygaocaolon8 07-11-2020 13 2   Download

Aspergillus oryzae, a safe filamentous fungus, is widely used in food and enzyme production. In this study, we examined a cultivation model using rice husks as carrier to assess the capacity of recombinant protein production in A. oryzae. The model was first tested with the A. oryzae strain expressing the DsRed reporter gene. The expression of DsRed was easily detected by the pink color of the fungal mycelium on culture media and under a fluorescence microscope. The model was then evaluated with the phyA gene encoding a phytase from the fungus Aspergillus fumigatus.

10p xilank_of 22-07-2020 19 1   Download

Phytase activity of bacteria, yeasts and moulds isolated from different ecosystems have been identified and studied by several researchers. The present study focused on isolation and identification of phytate solubilizing yeasts associated with freshly soaked cereals, pulses, condiments and oilseeds. Isolation on MRS-Calcium phytate at pH 6.2 ±0.2 resulted in a high diversity of microorganisms developing clear zones of phytate hydrolysis. Microscopic study revealed identification of three groups of phytate solubilizing organisms (viz.

9p trinhthamhodang6 09-07-2020 11 1   Download

This study explores the potential application of rice bran (agro waste) to nano-encapsulate phytase, which is a thermally unstable biologically active enzyme. Rice bran was converted to nanofbers (20–50 nm in diameter) using electrospinning.er composites for stabilization of phytase

7p vijiraiya2711 27-05-2020 11 0   Download

Phytase is used as an animal feed additive that degrades phytic acid and reduces feeding costs and pollution caused by fecal excretion of phosphorus. Some phytases have been expressed in Pichia pastoris, among which the phytase from Citrobacter amalonaticus CGMCC 1696 had high specific activity (3548 U/mg).

11p viuchiha2711 21-04-2020 6 1   Download

Magnesium (Mg) is the structural component of chlorophyll and polysaccharides and also an activator of several enzymes. Magnesium plays an important role in photosynthesis. An attempt was made in the present investigation on utilizing this property for increasing rate of photosynthesis of maize plant and subsequently higher root exudations which enhance the microbial activities in soil. Green house experiments were conducted to evaluate the effect of MgO nano particles spray on maize plant (Zea mays L.) in three benchmark soils of India which are phosphorus (P) deficient.

10p caygaocaolon2 11-03-2020 14 1   Download

Microbial phytases are of immense importance due to their application in food/feed industry by enhancing the availability of essential minerals such as phosphorous, iron, calcium etc. required for normal human physiology and also have commercial and environment significance. Therefore, in present study an attempt was made to enhance the production of phytase from isolated probiotic Pediococcus acidilactici BNS5B by employing both one variable at a time approach and statistically based design of experiments such as Plackett-Burman and Response Surface Methodology.

22p caygaocaolon3 09-03-2020 28 1   Download

In this study, an extracellular alkali-thermostable phytase producing bacteria, Bacillus subtilis B.S.46, were isolated and molecularly identified using 16S rRNA sequencing. Response surface methodology was applied to study the interaction effects of assay conditions to obtain optimum value for maximizing phytase activity. The optimization resulted in 137% (4.627 U/mL) increase in phytase activity under optimum condition (56.5 C, pH 7.30 and 2.05 mM sodium phytate). The enzyme also showed 60–73% of maximum activity at wide ranges of temperature (47–68 C), pH (6.3–8.

10p kequaidan1 16-11-2019 34 0   Download

In this study, the phytase from Lactobacillus brevis strains isolated from fermented food products was produced and characterized. Phytase production of this strain was determined by production of clear zones around the colonies on the sodium phytate containing medium, after 48 h of incubation at 30 °C.

9p vivant2711 01-02-2019 17 1   Download

The protein tyrosine phosphatase (PTP)-like phytase, PhyAsr, fromSeleno-monas ruminantiumis a novel member of the PTP superfamily, and the only described member that hydrolyzes myo-inositol-1,2,3,4,5,6-hexakisphosphate.

10p galaxyss3 07-03-2013 38 3   Download

The extracellular phytase of the plant-associatedKlebsiellasp. ASR1 is a member of the histidine-acid-phosphatase family and acts primarily as a scavenger of phosphate groups locked in the phytic acid molecule. The Klebsiellaenzyme is distinguished from theEscherichia coliphytase AppA by its sequence and phytate degradation pathway.

13p mobifone23 18-01-2013 45 4   Download