Phytoplasma infecting rice

Phytoplasmas are intracellular obligate prokaryotes that lack cell walls and have very small genomes (680–1,600 kb). Many fungal, bacterial and viral diseases have been characterized and resistant breeding programme is progressing very well. Recently, on the basis of the symptoms and on 16SrDNA sequence identity and phylogenetic relationships the association of ‘Ca. P. asteris’ with Rice Orange Leaf phytoplasma disease in South India was confirmed. Rice orange leaf phytoplasma was characterized based on multiple gene systems.

11p caygaocaolon2 14-03-2020 10 1   Download

DNA fingerprinting, estimation of molecular genetic diversity, marker assisted breeding and molecular diagnostic tools are the need of the hour for sugarcane crop improvement. Efficient extraction of genomic DNA (gDNA) free from polysaccharides, polyphenols, RNA and other major contaminants is a key to all these molecular activities. We present herewith, a modified DNA extraction protocol that has been optimized for isolating gDNA from endergonic and hardy sugarcane plant and its phytopathogens and endophytic fungi.

9p chauchaungayxua3 07-02-2020 9 0   Download