PIC values ranged

Genomic DNA was isolated by CTAB method and then subjected to PCR amplification by using 22 SSR primers. Among 22 primers, 17 primers were amplified. The PIC values were ranged from 0.46 (Phi046) which is the minimum value to 0.81(Phi059) which is the highest value. The frequencies of alleles were ranged from 0.01 to 0.07.

7p nguaconbaynhay7 15-08-2020 16 1   Download

Fusarium oxysporum f. sp. cumini is a serious disease of cumin (Cuminum cyminum L.). Ten isolates of F. oxysporum f. sp. cumini were isolated from major cumin growing districts of Gujarat state to compare molecular methods over traditional for differentiation among the isolates of pathogen. The average 98.70 % of polymorphism was recorded for all the 11 ISSR primers. The polymorphic information content (PIC) value ranged from 0.571 to 0.831 with an average of 0.739 per primer. The IISR primers grouped majority of the isolates based on their geographical location.

9p kethamoi6 29-06-2020 19 0   Download

A set of 45 polymorphic microsatellite markers, representing markers for almost each chromosome, was used for the assessment of genetic diversity in 35 genotypes of bread wheat. A total of 127 alleles were detected which ranged from 2 to 6 with an average allele number of 2.82 per locus. The PIC value of the 45 SSRs ranged from 0.08 to 0.50 with an average PIC of 0.30. Resolving power and Marker index of all 45 markers varied from 0.17 to 2.82 and 0.16 to 1.52, respectively.

11p quenchua6 15-06-2020 7 1   Download

The present study was undertaken to understand the genetic diversity of Chilli found in Manipur using RAPD primers. Twenty (20) primers were screened, out of which 9 primers gave polymorphic bands which were used for further analysis. The polymorphic information content (PIC) values ranged from 0.2 (OPB-15) to 0.45 (OPW-04) with an average of 0.35.

6p nguathienthan5 04-06-2020 19 1   Download

Molecular diversity analysis on 8 hybrids of tomato generated 27 polymorphic markers. The major allele frequency for the marker SSR1- 23.2 and SSR5-13.1 was least. The polymorphic information content value (PIC) ranged from 0.00 (SSR1-23.2) to 0.86 (SSR1-62.2) with an average PIC value of 0.46 per marker indicating more diversity at DNA level. Alleles ranged from 1-8 per locus with an average of 3.33 alleles per locus. Cluster analysis resulted into 3 main clusters and 4 subclusters. The diverse genotypes can be used for hybridization programmes.

6p quenchua4 06-04-2020 14 1   Download

Bread wheat is most cultivated cereal used as food over 95% of the population. Twenty wheat genotypes were assayed to study the genetic diversity using molecular markers. The seventy-five alleles were identified with a mean of 2.34 alleles per locus using 32 SSR markers. Majority of SSR markers showed a high level of polymorphism. PIC values ranged from 0.05 (WMS-169) to 0.75 (CWM-107), with an average of 0.38 per primer. The RP value of primer ranges from 0.92 (WMC-177) to 1.94 (WMS-169) with an average value of 1.51, which explains the ability of primers to resolve the studied germplasm.

10p quenchua4 06-04-2020 19 2   Download

Genetic diversity was performed among F2 plants of the cross RH 30×CS 52 in Indian mustard (Brassica juncea) (CS 52 is salinity tolerant and RH 30 is salinity susceptible) using SSR markers. Out of 358 SSR markers, 42 were found polymorphic and 154 were monomorphic. A total of 225 alleles, ranging from 2 to 4 were amplified. The PIC (Polymorphic Information Content) value ranged from 0.427-0.730 of Jaccard’s similarity coefficients was generated between these F2 populations. F2 population was grouped in 2 major clusters and 37 sub-clusters at a similarity coefficient of 0.58.

10p trinhthamhodang4 22-03-2020 18 0   Download

The genetic diversity and population structure in 51 chickpea accessions were studied using 30 chickpea specific SSR markers. Twenty eight SSR markers exhibited polymorphism producing a total of 217 alleles. The average number of alleles per locus was 7.75. The average PIC value was 0.75 and ranged from a minimum of 0.53 (TA1) to a maximum of 0.85 (TA64). The markers TA5, TA14, TA18, TA21, TA64, TA71, TA106, TR20, TR26, TR58, TS43 were considered to be highly informative (PIC ≥ 0.8), in evaluating allelic variation present in the chickpea accessions.

13p cothumenhmong4 25-03-2020 11 1   Download

Genetic diversity assessment is necessary to help tackle the threats of environmental fluctuations and for the effective exploitation of genetic resources in breeding program. Recent advancement in the field of molecular markers has made the genetic characterization of genotypes rapid, reliable and reproducible. In the present investigation, we have characterized 49 wheat genotypes at molecular level using 52 SSR primers (including Yr specific primers). 27 polymorphic SSR markers were dispersed over the AABBDD wheat genome, a total of 102 alleles were detected with allele range of 1 to 6.

12p caygaocaolon3 09-03-2020 14 0   Download

An experiment was carried out to study the divergence among twenty four rice genotypes (14 indica and 10 japonica lines) for 15 agro-morphological traits using D2 statistics and at molecular level using 114SSR markers. The cluster analysis using Mahalonobis’ D2 statistics classified the genotypes into five clusters. The cluster IV was the largest with seven indica and three tropical japonica types. The second largest cluster (V) accommodated four tropical japonica and two indica genotypes. The intra cluster distance ranged from 1.077 to 8.

12p trinhthamhodang3 12-02-2020 20 0   Download

Castor (Ricinus communis L.) is a plant that is commercially very important to the world. It is produced in about 30 countries lying in the tropical belt of the world. It is an important plant for production of industrial oil. Assessment of genetic diversity of a crop species is a prerequisite to its improvement; hence it is important to identify the genetic diversity of castor genetic resources for development of improved cultivars.

9p trinhthamhodang3 12-02-2020 15 0   Download

Information on allelic differentiation is very important to elucidate the nature and extent of divergence among Brassica juncea genotypes. In present study 20 Brassica juncea L. genotypes generated 79 polymorphic alleles from 15 SSR primer pairs with an average of 5.26 alleles per locus. The polymorphism percentage was recorded to be the maximum in the case of the primer Ni4- D04 (80%) and the minimum in the case of 3 different primers namely A06-20686249, Ni3-D03 and A05-20242013 (33.3%). The polymorphism information content (PIC) values ranged from 0.41 (A01-13393871) to 0.

11p chauchaungayxua3 07-02-2020 12 0   Download

Genetic diversity using Random Amplified Polymorphic DNA (RAPD) markers was assessed in forty eight Indian potato germplasm. Twenty RAPD markers amplified a total of ninety-one different loci that exhibited 82.30 per cent polymorphism among 48 potato germplasm. The PIC value ranged from 0.27 to 0.55. All the loci amplified by the primer which were found to be polymorphic varied in size from 1500bp. The Jaccard’s similarity coefficient was found to vary from 0.22 to 0.87. The maximum genetic similarity (0.87) was found between Kufri Badshah and Kufri Khyati and lowest (0.

9p chauchaungayxua3 07-02-2020 22 0   Download

Seed dormancy is an important trait affecting grain yield and quality in cereal crops. It is controlled by its own complicated genetic and environmental factors. The polymorphism pattern study of 24 rice genotypes for RM primers used in the present molecular study were clearly classified the rice genotypes into dormant (Cluster I, Cluster IIA) and nondormant (Cluster II B, Cluster III). In the present study, a total of 40 alleles were detected across the 24 rice genotypes by 11 polymorphic SSR markers. The number of alleles generated per locus by each marker ranged from 2 to 6.

11p nguaconbaynhay3 07-02-2020 11 0   Download

Wheat is qualitatively a vital source of macromolecule, energy and fiber for human nutrition since decades hence, used as a staple food grain for community and as well a major source of fodder for animal feeding. Assessment of genetic diversity using molecular markers is used for characterization of different genotypes with reliable and authentic results. RAPD is a PCR based molecular technique for identification of genetic variability in similar genotypes. It usually preferred for the initiation of this kind of work as this technique is simple, versatile and relatively inexpensive.

12p nguathienthan2 19-12-2019 15 0   Download

A total of 200 SSR markers from different Brassica species were used in this study. Out of 200 SSR markers analyzed for polymorphism in two parental Brassica juncea genotypes (RB 50, drought tolerant and Kranti, drought susceptible), 51 were polymorphic. The polymorphic markers were used to screen F2 population. A total of 108 alleles were identified in the RB 50 and Kranti and the parental B. juncea genotypes. The PIC (polymorphic information content) values for various primers ranged from 0.340-0.505 with an average of 0.406.

11p nguaconbaynhay1 04-12-2019 14 0   Download

The PCR products obtained from SSR analysis were separated on 3 percent high resolution agarose gel and the amplification patterns were observed. Eleven SSR primers which showed maximum polymorphism were selected for fingerprinting. The amplification pattern obtained with these primers were scored and depicted to develop fingerprint for each variety. Most of the amplicons were found to be shared among the genotypes. However, the pattern of sharing was different and good enough to separate out most of the varieties.

10p chauchaungayxua1 04-12-2019 13 1   Download