Plant genome engineering

Ebook "Tomato yellow leaf curl virus disease: Management, molecular biology, breeding for resistance" will awaken the interest of breeders, phytopathologists, environmentalists, extension services, plant virologists, entomologists and molecular biologists. It deals both with the epidemiological aspects of the disease and with integrated pest management in the field. It discusses the efforts aimed at breeding tomato plants resistant to the virus (using classical breeding, marker-assisted breeding and genetic engineering).

437p ladongphongthanh1008 22-04-2024 2 2   Download

Ebook "Salt stress in plants: Signalling, omics and adaptations" will shed light on the effect of salt stress on plants development, proteomics, genomics, genetic engineering, and plant adaptations, among other topics. The book will cover around 25 chapters with contributors from all over the world. ​​The interactions of salts with mineral nutrition may result in nutrient imbalances and deficiencies. The consequence of all these can ultimately lead to plant death as a result of growth arrest and molecular damage.

517p manmanthanhla0201 26-02-2024 5 1   Download

Ebook "Genetic engineering: Principles and methods (Volume 28)" presents state-of-the-art discussions in modern genetics and genetic engineering. This focus affirms a commitment to publish important reviews of the broadest interest to geneticists and their colleagues in affiliated disciplines. Recent volumes have covered gene therapy research, genetic mapping, plant science and technology, transport protein biochemistry, and viral vectors in gene therapy, among other topics.

206p manmanthanhla0201 27-02-2024 7 1   Download

Ebook "Site-directed insertion of transgenes" aims to present the state of this field from basic mechanisms of site-directed modifications to their applications in a wide range of organisms such as bacteria, yeast, plants, insects, mammals. It will discuss the problems encountered when using the random integration strategy and present the recent advances made in targeted genome modification.

393p nhanphanguyet 28-01-2024 3 2   Download

Ebook "Genomics and breeding for climate-resilient crops - Vol. 1: Concepts and strategies" presents the basic concepts and strategies for developing climate-resilient crop varieties. Topics covered include: conservation, evaluation and utilization of biodiversity; identification of traits, genes and crops of the future; genomic and molecular tools; genetic engineering; participatory and evolutionary breeding;...

555p lucchinguyen 28-12-2023 6 3   Download

Cotton is an essential fiber producing crop in the world. It also supports additional industries by providing high quality oil and protein in the form of cottonseed cake. Currently, there is an urgent need to increase lint yield, fiber quality, and resistance to biotic and abiotic stresses due to rising pressure from a global population and possible supply shortages from the effects of erratic climate changes. Classic plant breeding and transgenic strategies need more genetic breakthroughs to support the increasing pressure for fiber quantity and quality.

15p lyhuyenthu 31-01-2023 9 2   Download

Continued part 1, part 2 of ebook "Physiology and molecular biology of stress tolerance in plants" provide readers with content about: photooxidative stress; nutrient stress; heavy metal stress; metabolic engineering for stress tolerance; functional genomics of stress tolerance;... Please refer to the ebook for details!

187p hanlinhchi 11-11-2022 27 3   Download

Geminiviruses cause damaging diseases in several important crop species. However, limited progress has been made in developing crop varieties resistant to these highly diverse DNA viruses. Recently, the bacterial CRISPR/Cas9 system has been transferred to plants to target and confer immunity to geminiviruses.

10p vigalileogalilei 27-02-2022 11 1   Download

Increasing genetic diversity via directed evolution holds great promise to accelerate trait development and crop improvement. We developed a CRISPR/Cas-based directed evolution platform in plants to evolve the rice (Oryza sativa) SF3B1 spliceosomal protein for resistance to splicing inhibitors.

9p vigalileogalilei 27-02-2022 5 1   Download

The CRISPR/Cas9 system has recently been engineered to confer resistance to geminiviruses in plants. However, we show here that the usefulness of this antiviral strategy is undermined by off-target effects identified by deep sequencing in Arabidopsis. We construct two virus-inducible CRISPR/Cas9 vectors that efficiently inhibit beet severe curly top virus (BSCTV) accumulation in both transient assays (Nicotiana benthamiana) and transgenic lines (Arabidopsis).

7p vigalileogalilei 27-02-2022 28 1   Download

High-fidelity SpCas9 variants (eSpCas9 and SpCas9-HF1) have been engineered to reduce off-target effects. We found that changes in guide RNA length induced significant reductions in the editing activities of SpCas9 variants in plant cells.

7p vialfrednobel 29-01-2022 14 0   Download

Polyploidy is a pervasive evolutionary feature of all flowering plants and some animals, leading to genetic and epigenetic changes that affect gene expression and morphology. DNA methylation changes can produce meiotically stable epialleles, which are transmissible through selection and breeding.

14p vialfrednobel 29-01-2022 16 0   Download

The CRISPR/Cas9 system provides bacteria and archaea with molecular immunity against invading phages and conjugative plasmids. Recently, CRISPR/Cas9 has been used for targeted genome editing in diverse eukaryotic species.

11p viaristotle 29-01-2022 9 0   Download

Chloroplasts play a crucial role in sustaining life on earth. The availability of over 800 sequenced chloroplast genomes from a variety of land plants has enhanced our understanding of chloroplast biology, intracellular gene transfer, conservation, diversity, and the genetic basis by which chloroplast transgenes can be engineered to enhance plant agronomic traits or to produce high-value agricultural or biomedical products.

29p viaristotle 29-01-2022 11 0   Download

We describe here a CRISPR simultaneous and wide-editing induced by a single system (SWISS), in which RNA aptamers engineered in crRNA scaffold recruit their cognate binding proteins fused with cytidine deaminase and adenosine deaminase to Cas9 nickase target sites to generate multiplexed base editing.

15p viarchimedes 26-01-2022 11 0   Download

Cottonseed is one of the most important raw materials for plant protein, oil and alternative biofuel for diesel engines. Understanding the complex genetic basis of cottonseed traits is requisite for achieving efficient genetic improvement of the traits. However, it is not yet clear about their genetic architecture in genomic level. GWAS has been an effective way to explore genetic basis of quantitative traits in human and many crops. This study aims to dissect genetic mechanism seven cottonseed traits by a GWAS for genetic improvement.

17p vibeauty 23-10-2021 12 0   Download

Heterodera glycines, commonly referred to as the soybean cyst nematode (SCN), is an obligatory and sedentary plant parasite that causes over a billion-dollar yield loss to soybean production annually.

14p vigiselle2711 30-08-2021 7 1   Download

Traditionally, generation of new plants with improved or desirable features has relied on laborious and timeconsuming breeding techniques. Genome-editing technologies have led to a new era of genome engineering, enabling an effective, precise, and rapid engineering of the plant genomes.

12p vijichea2711 28-05-2021 11 1   Download

These technologies, the CRISPR/Cas9 system has gained more popularity by virtue of its simplicity, efficiency and reproducibility. CRISPR/Cas9 have been exploited to engineer plant virus resistance, either by directly targeting and cleaving the viral genome, or by modifying the host plant genome to introduce viral immunity. Here, we describe the biology of the CRISPR/Cas9 system and plant viruses, and how this genome engineering tool has been used to target the devastating plant viruses.

11p chauchaungayxua10 18-03-2021 6 2   Download

The recent advances in agricultural biotechnology and genetic engineering have brought numerous benefits to the food and agricultural sector by improving the essential characteristics of plant agronomic traits. Targeted genome editing using sequence specific nucleases (SSNs) provides a general method for inducing targeted deletions, insertions and precise sequence changes in a broad range of organisms and cell types.

15p chauchaungayxua11 23-03-2021 12 2   Download