Plant tissue culture

Around the world, plant tissue culture techniques have been successfully applied to conservation and clonal propagation of elite genotypes of numerous medicinal plants. Regenerated plants from Mentha spp...

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Production of secondary metabolites from plant tissue culture has emerged as a promising and feasible option attracting the attention of scientists worldwide. Plant cell, tissue and organ cultures offer an attractive alternative for homogeneous, controlled production of secondary metabolites, throughout the year, especially when we take commercial demand into account. They not only facilitate the de novo synthesis of novel compounds, but also are able to produce compounds sometimes even in higher amounts than the intact plants.

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Plant tissue culture has been widely employed in area of agriculture, horticulture, forestry and plant breeding. It is an applied biotechnology used for mass propagation, virus elimination, secondary metabolite production and in-vitro cloning of plants. Recently, plant tissue culture has been used for the conservation of endangered plant species through short and medium term conservation also known as slow growth and cryopreservation also known as long term conservation.

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Plant tissue cultures were obtained from the seeds of five varieties on the basis of petal colour) of Papaver somniferum and alkaloidal extracts were screend for the presence of secondary metabolites using thin layer chromatography and mass spectroscopy.

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Plant genetic transformation has become an important biotechnological tool for the improvement of many crops. A solid foundation for the fast development and implementation of biotechnology in agriculture has been provided by achievements in plant tissue culture. Plant tissue culture represents the most promising areas of application at present time and giving an out look into the future.

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Tài liệu Plant Propagation by Tissue Culture 3rd Edition Volume 1 - The Background for what you are learning about cell technology, a standard document in English, the opportunity to increase their ability to read English in biology.

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Banana (Musa spp.) is important fruit crop in India. Banana is one of the oldest and most popular fruit grown in India with great socio-economic significance. The present study aims to assessment of clonal fidelity and genetic analysis of tissue culture raised banana (Musa spp.) plant. Banana tissue culture propagation is a new an established a popular technology due to the advantages. Through plant tissue culture method large scale production is possible in short time.

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A frequent problem associated with the tissue culture of Compositae species such as chicory (Cichorium intybus L.) and lettuce (Lactuca sativa L.) is the premature bolting to in vitro flowering of regenerated plants.

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Plant tissue culture is the method of culturing the plant cells or tissues under controlled aseptic conditions on a solid or liquid medium. It has value in basic research for the production of true to type, disease free and micro-propagated plants. Besides this also has commercial application. This review work outlines the work done on pertaining to various aspects of in vitro micro-propagation, regeneration, rooting, hardening, in potato.

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Cucurbitaceae is the one of the elite family in the plant kingdom and have importance in its daily utilization for cuisine preparation as a source of vegetable and medicinal plant. This family consists of hundreds of edible species, qualitative and quantitative improvement plays a vital role in the processing industry and Indian medicine system of Ayurveda (AYUSH). Plant tissue culture techniques have been used extensively for propagation of cucurbitaceae by using various explants and methods from last few decades.

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Genetic transformation technology relies on the technical aspects of plant tissue culture and molecular biology for production of improved crop varieties, production of disease-free plants (virus), genetic transformation, production of secondary metabolites, production of varieties tolerant to salinity, drought and heat stresses

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Since the publication of this book, in 1983, several new and exciting developments have taken place in the field of Plant Tissue Culture, and it now forms a major component of what is popularly called Plant Biotechnology. Many of the important crop plants which were then regarded as recalcitrant are now amenable to regeneration from cultured protoplasts, cells, and calli, enabling subjection of these crops to improvement by biotechnological methods of cell manipulation.

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SPB2 2/27/2003 4:06 PM Page 35 2 Plant tissue culture Introduction Most methods of plant transformation applied to GM crops require that a whole plant is regenerated from isolated plant cells or tissue which have been genetically transformed. This regeneration is conducted in vitro so that the environment and growth medium can be manipulated to ensure a high frequency of regeneration. In addition to a high frequency of regeneration, the regenerable cells must be accessible to gene transfer by whatever technique is chosen (gene transfer methods are described in Chapter 3).

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Tissue culture techniques were applied for inducing callus of the red alga Kappaphycus alvarezii. The objective of this study was to obtain the optimal medium for callus induction from thallus explants of Kappaphycus alvarezii. Seaweed was collected from the Van Phong Bay (Khanh Hoa Province) and then was acclimatized in greenhouse and in semi-sterile culture in the laboratory.

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Plant tissue culture via somatic embryogenesis plays a key role in wheat genetic transformation studies. Therefore the production of embryogenic calli with a high regeneration capacity is a prerequisite for efficient plant regeneration.

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An experiment was conducted at Banana Research Centre, Mondouri under Bidhan Chandra Krishi Viswavidyalaya, Mohanpur, Nadia, West Bengal with five treatments and four replication at randomized block design. Two consecutive plant crop of cv. Grand Naine was planted during September of 2010 and 2011. Observations were recorded on various plant growth characters, yield and yield contributing characters.

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A field experiment was conducted on “Evaluation of Elite Tissue Culture Raised Banana Varieties for Growth, Yield and Quality under Bengaluru condition” during 2018 to 2020. The pseudostem continues to grow in height as the leaves emerge one after the other and reaches its maximum height when the the flower emerges at the top of the plant. It is a monocarpic and bears male, female and hermaphrodite type of flowers.

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The field experiment was conducted at College of Horticulture, Mudigere, to study on influence of planting density and fertilizers on growth and development of tissue culture banana cv. Ney poovan (AB) under hill zone of Karnataka during 2017-2018 with Factorial Randomized Block Design.

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The purpose of this book is to provide the advances in plant in vitro culture as related to perennial fruit crops and medicinal plants. Basic principles and new techniques, now available, are presented in detail. The book will be of use to researchers, teachers in biotechnology and for individuals interested to the commercial application of plant in vitro culture.

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In the present study, the effects of indole-3-butyric acid (IBA) separately and simultaneously with L-arginine on the morphogenic and biochemical responses in the cherry rootstocks CAB-6P (Prunus cerasus L.) and Gisela 6 (Prunus cerasus × Prunus canescens) were investigated.

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