Plant tissue culture

Plant tissue cultures were obtained from the seeds of five varieties on the basis of petal colour) of Papaver somniferum and alkaloidal extracts were screend for the presence of secondary metabolites using thin layer chromatography and mass spectroscopy.

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"plant propagation by tissue culture 3rd edition" volume 1 - the background for what you are learning about cell technology, a standard document in english, the opportunity to increase their ability to read english in biology.

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Since the publication of this book, in 1983, several new and exciting developments have taken place in the field of Plant Tissue Culture, and it now forms a major component of what is popularly called Plant Biotechnology. Many of the important crop plants which were then regarded as recalcitrant are now amenable to regeneration from cultured protoplasts, cells, and calli, enabling subjection of these crops to improvement by biotechnological methods of cell manipulation.

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SPB2 2/27/2003 4:06 PM Page 35 2 Plant tissue culture Introduction Most methods of plant transformation applied to GM crops require that a whole plant is regenerated from isolated plant cells or tissue which have been genetically transformed. This regeneration is conducted in vitro so that the environment and growth medium can be manipulated to ensure a high frequency of regeneration. In addition to a high frequency of regeneration, the regenerable cells must be accessible to gene transfer by whatever technique is chosen (gene transfer methods are described in Chapter 3).

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Tissue culture techniques were applied for inducing callus of the red alga Kappaphycus alvarezii. The objective of this study was to obtain the optimal medium for callus induction from thallus explants of Kappaphycus alvarezii. Seaweed was collected from the Van Phong Bay (Khanh Hoa Province) and then was acclimatized in greenhouse and in semi-sterile culture in the laboratory.

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Plant tissue culture via somatic embryogenesis plays a key role in wheat genetic transformation studies. Therefore the production of embryogenic calli with a high regeneration capacity is a prerequisite for efficient plant regeneration.

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The purpose of this book is to provide the advances in plant in vitro culture as related to perennial fruit crops and medicinal plants. Basic principles and new techniques, now available, are presented in detail. The book will be of use to researchers, teachers in biotechnology and for individuals interested to the commercial application of plant in vitro culture.

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In the present study, the effects of indole-3-butyric acid (IBA) separately and simultaneously with L-arginine on the morphogenic and biochemical responses in the cherry rootstocks CAB-6P (Prunus cerasus L.) and Gisela 6 (Prunus cerasus × Prunus canescens) were investigated.

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Silver nanoparticles (SNPs) are one of metallic nanoparticles widely applied in many fields. Research of SNPs application in plant tissue culture has been gaining attention in recent years. Moreover, novel plant tissue culture systems have been researched and developed for improving SNPs uptake capability in medium. In this study, we investigated effects of SNPs on Chrysanthemum morifolium ramat. cv. "JIMBA" growth and its ability in 3 culture systems: in vitro solid medium system, in vitro liquid medium system and microponic (combined of micropropagation and hydroponic).

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It is my privilege to contribute the foreword for this unique volume entitled: “Plant Tissue Culture Engineering,” edited by S. Dutta Gupta and Y. Ibaraki. While there have been a number of volumes published regarding the basic methods and applications of plant tissue and cell culture technologies, and even considerable attention provided to bioreactor design, relatively little attention has been afforded to the engineering principles that have emerged as critical contributions to the commercial applications of plant biotechnologies.

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Free radicals are constantly formed in living cells and removed by antioxidant defenses. Antioxidant enzymes are the main line of defense against free radicals in animal and plant cells. Uncontrolled generation ROS are involved in a number of human disease states, including diabetes and cancer due to disturbance in cellular and molecular processes including cell growth, differentiation and proliferation.

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Plant tissue culture is an efficient technique for conserving endemic plant species. A reproducible in vitro regeneration protocol was developed for the endemic Iris sari and Iris schachtii in the present study.

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Adventitious shoots were obtained from the diploid Diyarbakir watermelon cultivar "Sürme" by culturing cotyledons on shoot regeneration medium for 3 weeks. The effects of two cytokinins, benzyl amino purine (BA) and kinetine (Kin), on shoot organogenesis were examined. The number of shoots per explant were approximately more than 50% higher when 0.5 mg/l BA was used compared to the most effective kinetin concentration (1 mg/l).

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Flow cytometry measurement confirmed the genetic stability of Primula veris L. (cowslip) plants obtained during the micropropagation process. The mean 2C DNA content was 0.97 pg/2C and P. veris can be classified in the group of plants of very small genomes.

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Nuôi cấy mô, tế bào thực vật ( plant tissue culture) là kỹ thuật đưa một mô, bộ phận hoặc tế bào của thực vật vào trong một hệ thống vô trùng có thể kiểm soát về: thành phần chất khoáng, điều hoà sinh trưởng, các chất hữu cơ cung cấp cho cây.

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The clinical microbiology laboratory is often a sentinel for the detection of drug resistant strains of microorganisms. Standardized protocols require continual scrutiny to detect emerging phenotypic resistance patterns. The timely notification of clinicians with susceptibility results can initiate the alteration of antimicrobial chemotherapy and improve patient care. It is vital that microbiology laboratories stay current with standard and emerging methods and have a solid understanding of their function in the war on infectious diseases. ...

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There are several other reasons why sponges are a model laboratory organism to explore the biological consequences of environmental pollution. For the purposes of ease of set-up, freshwater sponges represent a cost- and time-effective study organism. Gemmules grow relatively quickly (within 3-5 days) and require very little equipment to grow. All of our sponges were grown in 24- well tissue culture plates.

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Mutation breeding techniques in combination with tissue culture and molecular marker methods provide a powerful tool for improvement of vegetatively propagated plants.

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The effects of mineral salts on fiber cell thickness in flax (Linum usitatissimum L. cultivar Sarı 85) were investigated in plants grown in medium both rich and poor in mineral salts and vitamins. Three media were investigated: Control (MS medium), mineral salt and vitamin deficient MS medium, and mineral salt and vitamin rich MS medium.

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The effects of sodium nitroprusside (SNP) on micropropagation of CAB-6P and Gisela 6 cherry rootstocks were investigated. Shoot multiplication of CAB-6P was reduced by adding 4.4 µM 6-benzylaminopurine (BA) and SNP.

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