Plate assay
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The thin-layer chromatography technique (TLC) is a simple and inexpensive analysis commonly used to identify qualitatively the presence of carbohydrates in food samples such as mono- di and oligosaccharides particularly. TLC assay could be improved using image processing software for the semiquantitative determination of this type of compound. In the present work, TLC-image analysis with Silica Gel 60 TLC plates was used for the semiquantitative determination of 6 standards of carbohydrates (glucose, fructose, sucrose, 1-kestose, nystose, and fructofuranosylnystose).
9p lyhuyenthu 31-01-2023 8 2 Download
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This study aimed to screen the sera of goats and sheep from flocks suspected of Mycobacterium avium subsp. paratuberculosis (MAP) infection by a newly standardized Mce-truncated ELISA (Mt-ELISA) kit for the detection of antibodies against MAP. Four diagnostic applied tests were evaluated including Indigenous plate-ELISA (IP-ELISA), Mt-ELISA, fecal Polymerase Chain Reaction (f-PCR) and fecal culture (FC).
9p vidarwin 22-02-2022 9 1 Download
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In the present work, the reaction of Cr(VI) with 1,5-diphenylcarbazide in microplates (enzyme-linked immunosorbent assay microtiter plate) and a subsequent determination in a plate reader at 540 nm were performed. The final volume utilized for each determination was 200 µL, and the cost and waste were reduced for hexavalent chromium [Cr(VI)] quantification. The use of microplates allowed for the analysis of a large number of samples, reducing analysis time.
6p langthannam 29-12-2021 5 0 Download
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The efficacy of different microbial strains for potassium dissolution from insoluble soil mineral (mica) were evaluated in vitro (both plate and broth media assays). Nine microbial strains and 0.1% of chemical source (mica) in three replications were used. Colony and halo diameters were measured after incubating the plates for 72h in incubator.
8p cothumenhmong9 04-01-2021 10 1 Download
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The study was conducted in Department of Microbiology RIMS, Raichur. Blood samples were collected from the patients attending orthopedic OPD of RIMS teaching hospital with complaints of fever, joint pain and backache. RBPT, MAT and ELISA IgM and IgG were carried out on serum samples.
9p gaocaolon9 22-12-2020 14 1 Download
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Staphylococcus epidermidis is a common commensal of the skin and mucous membranes of humans. However, it is a common cause of nosocomial infection especially infection associated with the insertion of biomedical devices. Glycans have been shown to mediate vast majority of biological processes and its alterations have been reported to be used as biomarkers and applied in diagnosis of malignancies and infections. The absolute mean Optical Density (OD450nm) of the biofilm was calculated as 0.248± 0.044SD and the biofilm was observed to strongly adhere to the microtitre plate surface.
10p caygaocaolon8 07-11-2020 9 2 Download
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The aim of this study was to detect biofilm formation by study isolates of Pseudomonas aeruginosa qualitatively and quantitatively. Ten isolates were taken from diseased animal in veterinary clinics of RAJUVAS Bikaner. Qualitative biofilm formation assays (Pellicle formation assay and Congo red agar method) and quantitative assay by Microtitre plate assay used for biofilm production.
6p nguaconbaynhay7 15-08-2020 13 1 Download
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Replant disease is a major problem of apple orchards, which is a main cash crop in TransHimalayan region. The cause of this replant problem includes both biotic and abiotic factors, such as low nutrients, phytotoxins, actinomycetes, fungal complexes and nematodes. Fungi and oomycetes belonging to the well-known root rot complex are important factor of replant problems in apple.
7p angicungduoc6 22-07-2020 9 1 Download
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Cellulose constitutes bulk of the plant cell wall materials and the most abundant and renewable non-fossil carbon source on earth. Cellulose degrading fungi play an important role in recycling of cellulosic materials. Thus, this study was designed to screen endophytic fungi isolated from Cameroonian medicinal plants for their cellulase activity. The plate-clearing assay and enzyme assay methods were used for screening of potent cellulolytic endophytic fungi. The promising was chosen for filter paper cellulase (FPCase) and carboxymethyl cellulase (CMCase) assay.
8p nguaconbaynhay6 24-06-2020 14 0 Download
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Objective of the research was to validate the potential of Syzygium aromaticum and Kaempheria galanga against biofilm formation and plasmid borne drug resistance exhibited by Pseudomonas spp. Antimicrobial property of S.aromaticum and K.galanga on P.aeruginosa culture was assessed using the agar well diffusion method. Crystal violet assay was employed to determine the percentage inhibition of P.aeruginosa biofilm by the extracts following generation of biofilm on multiwell plates.
7p nguaconbaynhay6 23-06-2020 30 0 Download
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Fibrinolytic enzymes constitute one of the most important groups of industrial enzymes Aspergillus japonicum KSS 05 strain were used for fibrinolytic enzyme production. The potential strain were kept for mutational studies. UV radiation is used as mutagenic agent. The mutant obtained i.e Aspergillus japonicum KSS 05 mu were used for screening of fibrinolytic enzyme production by plate assay and it showed 6 mm diameter clear zone around the colony.
4p nguaconbaynhay6 23-06-2020 9 0 Download
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An experiment was conducted for isolation of cellulolytic microorganisms from diverse environments. Samplings were done from 8 locations representing 27 samples viz. forest ecosystem, composting units, incorporation experiments and agricultural fields. A total of 653 microorganisms including bacteria, actinomycetes and fungi were isolated using serial dilution plate assay. All the cultures were initially screened for cellulolytic potential using carboxy methyl cellulose medium on the basis of zone of hydrolysis obtained.
11p cothumenhmong5 17-05-2020 14 0 Download
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The aim of the study was to access the anti-bacterial and anti-fungal activity on the growth of Alternaria porri under in vitro condition. The antagonistic activity of bacterial and fungal antagonists (Pseudomonas and Trichoderma spp.) was determined by the dual culture technique both on King’s B and PDA media respectively, Paper disc assay and Agar well mathod. Plates were incubated at 28ºC for 7 days and 2 days. The antagonistic activity of these antagonists was evaluated by mycelial growth and mycelial dry weight of test fungus (Alternaria porri).
6p chauchaungayxua5 08-05-2020 16 0 Download
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Grass pea (Lathyrus sativus) is an underutilised crop with high tolerance to drought and flooding stress and potential for maintaining food and nutritional security in the face of climate change.
14p viharuno2711 21-04-2020 15 2 Download
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Sixty one out of 200 isolates, isolated from chickpea rhizospheric soils, were found to be effective to control the mycelial proliferation of the test pathogen Fusarium oxysporum f. sp. ciceris, maximum being with Ps16b (45.7%) and Ps45 (87.3%) in dual culture plate and liquid broth assay. Growth inhibition of the phytopathogen was also recorded by diffusible and volatile antifungal metabolites produced by the isolates. All the isolates showed ammonia production, whereas only nine isolates were recorded for Hydrogen cyanide production.
22p caygaocaolon4 01-04-2020 10 1 Download
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The present study was undertaken to ascertain the presence of anticancer phyto-constituent in the Indian Combretum species and its further utilization in the treatment of tumor. The phyto-chemical constituents of the leaf and bark extracts were recognized with reference to standard on the same HPTLC plate. The leaf extracts exhibited higher quantity of combretastatins than the bark samples. The combretastatin content was recorded the highest (182 µg/g) in the leaf extract of C. albidum. Antibacterial activity was studied by disk diffusion assay against seven bacterial strains.
13p trinhthamhodang4 22-03-2020 9 0 Download
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Brucellosis is a zoonotic disease with epidemiological global effect. It has been found to affect the cattle industry worldwide and India is not an exception to it. The current sero survey was carried to illuminate the status of bovine brucellosis in an organised dairy farm from Nasik with past history of abortions. Total of 65 serum samples were collected from cattle and tested for the presence of brucella antibodies using Rose Bengal Plate Test (RBPT), Standard Tube Agglutination Test (STAT) and Indirect Enzyme Linked Immnuno-Sorbent assay (I-ELISA).
6p caygaocaolon3 09-03-2020 8 0 Download
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In recent decades the global emergence the development of multidrug-resistant and pan drug resistance in Pseudomonas aeruginosa has been observed. This opportunistic pathogen is responsible for causing various infections especially in the intensive care units, resulting for almost 10% of nosocomial infections. The resistance to Metallo betalactamase in P. aeruginosa strains has been related with bacterial biofilm formation. So, the aim of this study was to determine the rates of biofilm production and metallo betalactamase production (MBL) among the strains of Pseudomonas aeruginosa.
6p trinhthamhodang3 22-02-2020 8 0 Download
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Metallo beta-lactamase (MBL) and biofilm production is the most worrisome resistant mechanisms observed in Pseudomonas aeruginosa. Increased resistance of Pseudomonas to antibiotic therapy is an emerging global health concern which can be overcomed by Silver nanoparticles (AgNPs). Therefore this study aims to evaluate the antibacterial and antibiofilm activity of Silver nanoparticles on MBL and biofilm producing P. aeruginosa isolates using Transmission Electron microscope (TEM) and Confocal laser scanning microscope (CLSM) respectively. A total 40 isolates of p.
18p chauchaungayxua3 07-02-2020 11 0 Download
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In this study, antagonistic effect of 6 isolates of Bacillus subtilis (Bs-1, Bs-2, Bs-3, Bs-4, Bs-5,Bs-6), Pseudomonas fluorescens (Pf-1, Pf-2, Pf-3, Pf-4, Pf-5, Pf-6), Saccharomyces cerevisiae (Sc-1, Sc-2, Sc-3, Sc-4, Sc-5, Sc-6) and Trichoderma spp. (Ts-1, Ts-2, Ts-3, Ts4, Ts-5, Ts-6) were evaluated against Colletotrichum capsici, the causal agent of chilli fruit rot, as potential biocontrol agents under in vitro conditions. Fungal and bacterial biocontrol agents were tested against chilli fruit rot pathogen (C.capsici) by dual culture plate assay.
9p quenchua2 16-12-2019 7 0 Download