Poisoned food technique
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To study the effect of different fungicides against Alternaria leaf spot of ber, fungus was isolated from the infected leaves of ber plant and cultural and morphological study was done through Poison Food Technique to find that the fungus was Alternaria alternata having branched and septate mycelium with light brown to blackish conidiophores in acropetal chain and the conidia having olive green to dark brown colour with transverse and longitudinal septa.
6p trinhthamhodang11 27-04-2021 15 1 Download
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Effect of metarhizium anisopliae on rhizoctonia solani and meloidogyne graminicola in rice seedlings
An experiment was conducted in the laboratory of Department of Plant Pathology, SHUATS, Prayagraj, U.P. during 2019-2020 to observe the effect of 15 botanical extracts against Rhizoctonia solani by Poison Food Technique.
9p trinhthamhodang11 27-04-2021 7 1 Download
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The present in vitro study was carried out at the Department of Plant Pathology, University of Agricultural Sciences, Dharwad, Karnataka, India to evaluate the efficacy of different fungicides against Colletotrichum lindemuthianum (Sacc. & Magn.) Bri. & Cav. causing anthracnose of french bean using poisoned food technique.
7p caygaocaolon11 21-04-2021 12 1 Download
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In-vitro compatibility studies of B. bassiana (Bb-5a) with ten insecticides were examined on PDA media by poisoned food technique to develop suitable combinations for the control of whitefly in sunflower ecosystem. Out of ten insecticides, nine insecticides viz., imidacloprid, thiamethoxam, acetamiprid, clothianidin, acephate, fipronil, buprofezin, flonicamid and azadirachtin showed compatible with B. bassiana at recommended dose (RD), half of the recommended dose (0.5 × RD) and double the recommended dose (2 × RD) at 14th and 30th DAI.
11p caygaocaolon11 21-04-2021 13 1 Download
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Selected essential oils namely clove bud oil, lemongrass oil, grapefruit oil, lemon oil and rosemary oils studied for their composition and antifungal activity against the B. cinerea a significant postharvest pathogen. The compositional analysis by FT-IR revealed the several characteristic peaks corresponding to the several aromatic compounds of essential oils. Major compounds were identified for each essential oil as eugenol in of clove bud oil, citralin lemongrass oil, β-myrcene in grapefruit oil, limonene in lemon oil and camphor in rosemary oil.
10p chauchaungayxua10 19-03-2021 14 2 Download
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Rapeseed-mustard belongs to family Brassicaceae. It is the most important group of rabi oilseed crops and contribute a major share to the vegetable fat of the country. Effectiveness of the six fungicides i.e. Quintal (Iprodione 25% + Carbendazim 25%), Nativo (Tebuconazole 50% + Trifloxystrobin 25% 75GW), Tilt (Propiconazole 25 EC), Score (Difenoconazole 25 EC) and Propineb (Antracol 70 WP), Folicur (Tebuconazole 250 EC) were used at three concentrations of each @ 0.05, 0.1 and 0.2 per cent and one is no treatment (control) under in vitro against Alternaria brassicae by poison food technique.
5p trinhthamhodang9 16-12-2020 11 1 Download
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Pigeonpea wilt disease caused by Fusarium oxysporum f. sp. udum is one of most devasting seedborne disease. In this experiment total eight seed dressing fungicides at their recommended dosages were evaluated in-vitro by poisoned food technique, against Fusarium oxysporum f. sp. udum causing wilt.
5p trinhthamhodang9 16-12-2020 8 1 Download
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Six fungicides viz., captan 50 WP, copper oxychloride 50 WP, dodine 65 WP, mancozeb75 WP, propineb70 WP and zineb 80 WP were evaluated against rice sheath blight (Thanatephorus cucumeris). The in-vitro evaluation of these fungicides through poisoned food technique at five different concentrations viz., 50, 100, 200, 500 and 1000 µg a.i ml-1 indicated that mancozeb proved the most effective exhibiting mean mycelial growth inhibition of 95.26 per cent followed by captan and dodine with 93.80 and 93.66 per cent, respectively. Copper oxychloride proved least effective and resulted in only 44.
9p caygaocaolon8 07-11-2020 9 2 Download
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In vitro screening of different contact, systemic and combi-products against Alternaria tenuissima the fungus causing leaf blight of kodo millet was done employing poison food technique. The results revealed that, among the four contact fungicides, mancozeb at 0.15 and 0.20 per cent concentration gave the maximum inhibition (69.33 and 66.67%) of mycelial growth.
6p angicungduoc8 07-11-2020 16 1 Download
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An in vitro bioassay was undertaken to record the compatibility of local isolate of Trichoderma harzianum with fungicides used in coorg mandarin-pepper-coffee plantations. Eight non-systemic, 10 systemic and 9 combi products fungicides were evaluated against Trichoderma for radial growth inhibition on PDA medium using poisoned food technique. Contact fungicides at selected concentration were found to be safer than systemic and combi products except Chlorothalonil.
9p nguathienthan8 20-10-2020 14 2 Download
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Fusarium oxysporum f. Sp lycopersici is the causal organism that causes wilt disease in tomato all over the world. The antifungal activity of aqueous extract of neem leaf (5%), ginger bulb (5%), Lantana camara (5%), Trichoderma harzianum (5%), carbendazim (0.01%) and combination of carbendazim+T. harzianum (0.01+5%) was investigated against Fusarium oxysporum f. sp lycopersici in vitro.The least growth of pathogen was recorded in Carbendazim (treated control) (94.00) followed by Neem leaf (35.20) followed by Lantana camara (31.11), ginger bulb (26.
6p nguathienthan8 20-10-2020 17 1 Download
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Among the silkworm diseases, white muscardine caused by Beauveria bassiana is the most common disease. The seven different chemicals viz., BKC (90%), pine Oil (100%), bleach (12%), sodium chlorite (80%), bleaching powder (30%), sanitech (2000ppm) and hydrated lime (80%) were tested and screened against fungus (beauveria bassiana and bacteria (bacillus and staphyllococcus) based on poison food technique with different concentrations such as 0.05%, 0.5%, 1%, 2%, 2.5%, 3%, 5%and 10% with three replications for each chemical concentration along with control.
13p nguaconbaynhay8 13-10-2020 15 2 Download
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Now these days, Plant extracts are used to control plant diseases because of their eco-friendly nature and cost effectiveness. The present investigation focuses on the antifungal activity of eight plant extracts determined through in vitro following poisoned food technique. Maximum inhibition of P. infestans was obtained by Salix sp. leaf extract (59.80% reduction) followed by Datura (48.47%). The other tested plant extracts exhibited moderate activity.
6p trinhthamhodang1215 23-09-2020 8 1 Download
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The bacterial leaf blight of rice caused by Xanthomonas oryazae pv. oryzae is the disease of great economics importance in India especially in Bihar. Management of BLB disease can play a vital role in management of rice disease. In the present investigation evolution of different plant extract (botanical) and new chemical on growth of bacteria by poison food technique and sowing by best botanical and new chemical were conducted and antagonistic activity were tested.
7p trinhthamhodang7 31-08-2020 7 1 Download
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For this study, disease infected leaf samples were collected from pulse pathology block situated at Normon E. Borlaug Crop Research Centre, Pantnagar based on cultural, morphological and disease potential studies and then evaluated the pathogen against five botanicals viz. Calotropis, Ocimum, Aloevera, Neem and Bael at 5, 15, 25 and 35 per cent concentration through poison food technique and two bioagents viz.
10p nguaconbaynhay7 15-08-2020 31 1 Download
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In vitro evaluation of twelve phytoextracts (aqueous and methanolic extract) of locally available plants having antimicrobial properties were done against Rhizoctonia solani, Kuhn by sequentially highering the concentration of the extracts i.e. at 1%,2.5%, 5% and 10%. The poisoned food technique employed for the purpose.
9p nguaconbaynhay7 15-08-2020 13 2 Download
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Chickpea (Cicer arietinum L.) is one of the major legume pulse crops and it is majorly grown in India and other semi-arid regions of the world. Dry root rot of chickpea is the most destructive disease of chickpea. Therefore, in present studies fungicides (six systemic, seven non systemic and six ready mix fungicides) were tested in vitro at three different concentrations by poisoned food technique for evaluating their efficacy against M. phaseolina.
11p nguaconbaynhay7 15-08-2020 11 1 Download
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In the present investigation 30 Plant leaf extract were evaluated in in vitro condition against P. palmarum adopting poisoned food technique. The per cent inhibition of pathogen was 100 per cent by Dhatura, Anjwain and Tobacco at 10 per cent concentration followed by the turmeric (95.84), safed musli (90.62), garlic (87.5), hathjodh (86.5), kalmegh (86.46), jetropha (83.34), neem bark (79.18), satavar (78.12), lemongrass (69.81), laung (67.71), ashoka (65.62), aadusa (63.68), karanj bark (63.06), bhringraj (62.5), karanj (62.5), dalchini (61.15), beshram (60.43), brijdanti (57.
7p angicungduoc6 22-07-2020 10 1 Download
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Six fungicides viz., Tebuconazole + Trifloxystrobin 75 WG (Nativo), Hexaconazole 5% + Captan 70 % WP (Taqat), Carboxin 37.5% + Thiram 37.5% WP (Vitavax power), Iprovalicarb 5.5% + Propineb 61.25%WP (Melody duo), Carbendazim 50WP (Hycarb), Mancozeb 63% W.P. + Carbendazim 12% WP (Top too) were evaluated in vitro to test their efficacy against Macrophomina phaseolina by poisoned food technique. Results revealed that all the fungicides were effective in inhibiting mycelial growth of M. phaseolina to varying degrees.
7p angicungduoc6 22-07-2020 7 1 Download
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To manage the disease an investigation at the Department of Plant Pathology, SHIATS-DU, Allahabad was carried out to evaluate the effect of bio-agents viz. Trichoderma viride @ 2%, Pseudomonas fluorescens @ 2%, T. harzianum @ 2%, botanical viz. neem leaf extract @ 5%, neem oil @ 5% and fungicide viz. carbendazim @ 0.2% against R. solani under invitro condition by dual culture and poisoned food technique. All the bioagents and botanical were evaluated singly. All the treatments significantly inhibited the mycelial growth of R. solani as compared to untreated check.
5p chauchaungayxua6 26-06-2020 11 2 Download