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Polyacrylamide gel electrophoresis
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Ebook "Difference gel electrophoresis: Methods and protocols" provides a comprehensive update of this key method on gel-based proteomics. Chapters present an introduction into the development of methods on principles of differential protein labeling and two-dimensional gel electrophoresis, techniques on optimized proteomic workflows using advanced mass spectrometry for protein identification, and the application of those methods in basic biological research, pathobiology and applied biomarker discovery.
310p
lucchinguyen
28-12-2023
6
2
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Virus coat protein (CP) molecular weight was determined using sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE), giving a single band of 25 kDa within resolving gel. Immunocapture-reverse transcriptase-polymerase chain reaction
5p
schindler
30-12-2021
5
0
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In this study, some enzymology parameters and some antibiotics affecting the enzyme activity of 6- phosphogluconate dehydrogenase (6PGD) extracted from t the livers of fish from Lake Van (Chalcalburnus tarichi) were investigated because it is an important enzyme-producing NADPH, a reductive power, protecting the cell against the oxidative agents by producing reduced glutathione. The crude enzyme solution was obtained by using the affinity chromatography method.
9p
langthannam
29-12-2021
11
0
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Two-dimensional polyacrylamide gel electrophoresis (2D PAGE) is commonly used to identify differentially expressed proteins under two or more experimental or observational conditions.
14p
viwyoming2711
16-12-2020
18
1
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The protein bands in ostrich seminal plasma (OSP) were identified by using Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE) and Matrix Assisted Laser Desorption Ionization Mass Spectrometry (MALDI MS) proteomic analysis and compared with NCBI reference sequence.
6p
caygaocaolon8
07-11-2020
15
2
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Thirteen (Three gynoecious and ten monoecious) germplasm lines of cucumber (Cucumber sativus L.) were characterized by sodium dodecyl sulphate polyacrylamide vertical slab gel electrophoresis (SDS-PAGE). The seed protein could be resolved into total 11 bands distributed in 4 zones i.e. A, B, C and D. Zone A was divided into 5 subzones and 5 bands, zone B has 1 band C has 5 and zone D included 5 bands. Similarity index value ranged from 62% to 100% among all the genotypes. Pgyn-1 showed least similarity 68% with other genotypes.
9p
angicungduoc5
12-06-2020
9
0
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Habitat fragmentation is a serious threat to species survival. In Vietnam, Cycas balansae has been considered as threatened species because of the reduction and fragmentation of its habitats and over-exploitation. We assessed genetic variability and the pattern of population structure among six populations sampled in four provinces: Hoa Binh, Ha Nam, Ninh Binh and Quang Ninh. Polyacrylamide gel electrophoresis was performed on leaf tissues from 152 individuals representing 6 populations of C. balansae.
13p
12120609
01-06-2020
13
0
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Bovine dermatophilosis, also known as cutaneous streptothrichosis in cattle, is a skin infection which causes severe production losses in tropical countries. The present paper reports protein profile analysis of D. congolensis isolates from bovines in Kerala and identification of the immunodominant proteins using western blotting. Fifteen isolates of Dermatophilus congolensis obtained from dermatitis cases from 14 cattle and one buffalo were included in the study.
6p
caygaocaolon5
27-05-2020
11
2
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Six isolates of chickpea collar rot fungus of Sclerotium rolfsii were collected from different locations of Maharashtra were investigated for genetic diversity under present experiment. We employed five SSR of MB- series to construct a genotype-specific DNA fragment profile of field isolates of this fungus. The PCR amplified product of each primer was resolved on 10 % Polyacrylamide gel electrophoresis. The 5 SSR primers screened produced a total of 60 reproducible and scorable amplicons. The size of amplicons produced ranged from 115bp to 800bp.
8p
chauchaungayxua5
05-05-2020
17
2
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In the present study, SDS-Polyacrylamide gel electrophoresis was used to separate protein sub units of albumin and globulin under reducing conditions to study the induction or disappearance during inhibition of germination. The molecular masses of the albumin and globulin protein subunits were estimated in relation to the motilities of the standard molecular mass proteins (Bio-Rad). However the SDS PAGE resolution of seed albumin in Pusa vishal and K851 showed 9-13 band corresponding to 110-5.
12p
angicungduoc4
26-04-2020
16
2
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Isozyme analysis/isozyme patterns of esterases and peroxidase of twenty eight isolates of the pathogen R. solani collected from maize (27) and one isolate from rice were used to study the variability among the isolates of R. solani through Polyacrylamide Gel Electrophoresis (PAGE) ) and polyphenol oxidases by Spectrophotometer.
18p
cothumenhmong4
23-03-2020
13
1
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The objective of the research was to study the protein content and electrophoretic profile of specific fractions of Large White Yorkshire (LWY) boar semen. Sperm rich fractions (SRF) of semen were collected from four sexually mature LWY boars as fractions of 10 mL each, by gloved hand method. For analysis, the first 10 mL of the SRF, rest of the SRF and sperm free fraction (SFF) were collected separately.
6p
cothumenhmong3
22-02-2020
13
1
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Serum immunoinflammation-related protein complexes (IIRPCs) and diabetes-related protein complexes (DRPCs) in 1537 serum samples including 504 healthy controls, 320 patients with prediabetes, and 713 patients with T2DM were analyzed using an optimized native polyacrylamide gel electrophoresis (native-PAGE).
7p
vinasaki2711
12-11-2019
7
1
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Polyacrylamide gel electrophoresis was employed to study the isoenzyme variation of esterase and acid phosphatase in natural populations of Dasypyrum villosum (L.) P.Candargy, Elytrigia repens (L.) Nevski and Elymus caninus (L.) L. Four similarity indices (SI, S, D, Ih) were calculated in an attempt to evaluate quantitatively genetic affinities among the species examined.
6p
viminotaur2711
30-10-2019
14
0
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The present study was undertaken to determine the relationship between MethicillinResistant Staphylococcus aureus (MRSA) strains isolated from various clinical specimens in Gülhane Military Medical Academy, in Ankara, Turkey.
8p
caplock2711
19-02-2019
23
1
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Native-polyacrylamide gel electrophoresis (N-PAGE) was used to characterize Candida albicans strains isolated from vaginal specimens of patients suffering from vaginitis in Ankara hospitals, Turkey.
6p
caplock2711
19-02-2019
28
0
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There are no reports in the literature dealing with gram-positive cocci typing by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of extracellular proteins. In this study, Staphylococcus aureus, Staphylococcus warneri, Staphylococcus chromogenes, Micrococcus lysodeikticus and Micrococcus ssp. of the Micrococcaceae family, and Pediococcus halophilus, Streptococcus ssp. and Enterococcus faecalis of the Streptococcaceae family were characterized by SDS-PAGE of whole cell proteins (WCPs) and total extracellular proteins (TEPs).
7p
caplock2711
19-02-2019
23
0
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In this study, 18 live specimens collected from six localities in the distribution areas of Mesocricetus brandti and Mesocricetus auratus were examined using SDS-PAGE (sodium dodecyl sulphate-polyacrylamide gel electrophoresis). The findings showed that there is no difference in globulin and albumin proteins of M. brandti and M. auratus, and supported the claim that M. auratus is very closely related to M. brandti.
4p
caplock2711
19-02-2019
16
0
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The blood serum proteins of 35 live specimens of Rattus rattus and Rattus norvegicus collected from ten localities in Turkey were examined by SDS-PAGE (sodium dodecyl sulphate- polyacrylamide gel electrophoresis).
6p
caplock2711
19-02-2019
20
0
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Blood serum proteins of Apodemus flavicollis and Apodemus hermonensis (Mammalia: rodentia) in Turkey
The globulin, albumin, postalbumin, and prealbumin proteins of Apodemus flavicollis and Apodemus hermonensis were examined by SDS-PAGE. In A. flavicollis and A. hermonensis respectively, 6-9 and 7-9 electrophoretic bands were determined in the globulin region, respectively.
4p
caplock2711
19-02-2019
18
0
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