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Polyclonal antibody
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Tetracycline (TC) is a broad-spectrum antibiotic used in the treatment of several infectious diseases. The excessive use of tetracycline antibiotics, especially tetracyclines in livestock, has raised concerns about residues of tetracycline antibiotics in food that may affect consumers' health.
12p
viamancio
04-06-2024
2
0
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In this study, new polyclonal antibodies detecting four TGM2 variants encoding protein from ENSEMBL database are produced and their specificities are confirmed by western blot analysis with E.coli overexpressing TGM2-002 protein, HEK293T cells overexpressing TGM2-S protein and human colon cancer samples.
11p
vimarillynhewson
02-01-2024
6
2
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The aim of this work was to develop a competitive enzyme immunoassay technique, to detect the presence of traces of soy in meat products. Specific rabbit polyclonal antiserum against soy protein was used as primary antibody.
6p
viisac
23-09-2023
3
1
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Enhanced Green Fluorescent Protein (eGFP) shows much stronger fluorescence than its ancestor, Green Fluorescent Protein (GFP), thus has been widely applied as a reporter for biomedical research. In this study, we reported the expression of a synthetic codon optimized gene encoding eGFP in Escherichia coli (E. coli).
10p
vimulcahy
18-09-2023
4
3
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Southern rice black-streaked dwarf virus (SRBSDV) causes severe epidemical disease on rice with the infected area up to millions of hectares in South China and North and Central of Vietnam. So far, there are no effective, cheap, quick, and practicable methods for diagnosing SRBSDV. The conventional RT-PCR technique is the most popular method for detecting SRBSDV with high accuracy.
9p
viengels
25-08-2023
2
2
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Klebsiella pneumonia, a Gram-negative bacterium belonging to the genus Enterobacter, causes many human and livestock diseases. Notably, infected goats may develop pneumonia, septicemia, which can lead to occasional death, resulting in great economic losses in goat-farming industry. However, there are little systematic methods for detection of goat Klebsiella pneumoniae in livestock production.
10p
vidarwin
23-02-2022
12
1
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The possible use of oxytocin in saliva as an indicator of positive emotions in bovine species has been poorly investigated. In the present study, two new assays (one using a monoclonal antibody and the other using a polyclonal antibody) for the measurement of oxytocin in bovine saliva were developed and validated. Also, the changes in oxytocin in saliva were explored in two different situations.
8p
vidarwin
23-02-2022
12
1
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In this study, an anti-MSA polyclonal antibody (pAb) was generated in rabbit with titers of 1:2 700 000 and purified by nProtein A affinity chromatography with purity greater than 90%. The purified antibody was conjugated to horseradish peroxidase (HRP) by the m-periodate method with an optimal working dilution of 1:64 000 in direct ELISA.
5p
mudbound
10-12-2021
8
1
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The present study was taken up in order to develop polyclonal antibodies against both the pathogens, for the serological detection of their presence in fruits before fruit ripening.
8p
chauchaungayxua11
23-03-2021
7
1
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Listeriosis caused by Listeria monocytogenes is an infectious and zoonotic disease affecting wide range of animals including ruminants. An outbreak of listeriosis was investigated in migratory flock of sheep and goat in Punjab. Outbreak of encephalitic listeriosis in sheep was diagnosed by characteristic clinical signs, histopathology and confirmed by Immunohistochemistry (IHC). Microscopically, there were multifocal areas of aggregation of neutrophils forming microabscesses a pathognomic lesion of the disease.
4p
nguaconbaynhay7
15-08-2020
9
1
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Cloning and expression of recombinant VP2 capsid protein gene of canine parvovirus in E. coli System
In the present study, new CPV-2a field strain (KLD3) isolated in cell culture was selected and the whole CPV VP2 gene was used for the expression in the E. coli expression system. Prokaryote expressing monocistronic DNA cassette containing open reading frame of whole CPV capsid gene (VP2) downstream of T7 promoter was synthesized. Analysis of the expression in E. coli cells showed the presence of capsid protein. Recombinant capsid protein showed immunoreactivity similar to the whole CPV virus antigen, when reacted with polyclonal antibodies against the whole CPV virus particles.
10p
angicungduoc5
12-06-2020
5
1
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Purified viral protein was obtained from Potato virus Y (PVY) pure culture maintained on potato (Solanum tuberosum L.) plants. The average concentration of the purified materials was found to be 153.1 ng/µl. PVY purified virus preparation was used for immunizing rabbit for production of polyclonal antisera against the virus. Good quality antisera were collected one week post boosters (AS4b, AS5b, AS6b and AS7b) and the IgG fractions were separated through ammonium sulphate precipitation.
6p
cothumenhmong5
17-05-2020
14
1
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Dehydration-responsive element-binding (DREB) proteins play a critical role in the plant’s droughttolerance mechanism despite their presence in minor amounts in the cell. In this study, a maize-derived transcription factor protein, ZmDREB2.7, was overexpressed in the Escherichia coli strain Rosetta 1. The interested gene conjugating with the thioredoxin gene (TrxA) and his6 tag in the pET-32a vector encoded a 55.7 kDa fusion protein. The optimum condition for inducing the thioredoxin-his6 -ZmDREB2.7 expression was five hours of induction with 0.05 mM IPTG at 300 C. The Tris-HCl 20 mM pH 8.
7p
caygaocaolon1
13-11-2019
11
0
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The antibody titer and specificity were determined by ELISA and western blot analysis, respectively. The tissue distribution of pSix1 was determined by western blot using the prepared polyclonal antibody.
8p
vikimsa
22-02-2019
17
1
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Moroctocog alpha is a human B-domain deleted recombinant factor VIII (BDDrFVIII), which represents a new generation of pure antihemophilic products. We describe here an optimized procedure for polyclonal anti-FVIII-antibody production with the use of BDDrFVIII as an immunogen.
11p
nutifooddau
27-01-2019
23
1
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In this study, the direct tissue blot immunoassay (DTBIA) procedure was tested for the detection of 10 different Turkish isolates of the citrus tristeza virus (CTV) by employing monoclonal and polyclonal antibodies.
7p
aquaman27
15-01-2019
15
1
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The properties of recombinant VP6 protein were characterized by Western blot and ELISA methods. Pure recombinant VP6 protein was used as an antigen to inject into the rabbits. The recombinant VP6 protein was highly immunogenic in rabbits. Anti-VP6 antibodies were purified from the rabbit serum using the Protein A affinity chromatography.
9p
cumeo3000
01-08-2018
23
0
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In this study, we present the development of a lateral flow immunoassay for detection of rotavirus using polyclonal antibodies.Rotavirus is the most common cause of severe, dehydrating diarrhea in children worldwide.
7p
jangni2
19-04-2018
36
1
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Polyclonal antibody against the DPV UL46M protein can be a diagnostic candidate
10p
dauphong3
26-12-2011
40
4
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Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Production, purification and characterization of polyclonal antibody against the truncated gK of the duck enteritis virus
7p
dauphong2
23-12-2011
46
3
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