Potentiometry

A comparative study of thermodynamic and kinetic aspects of Cu(II) and Ni(II) binding at the N-terminal binding site of human and bovine serum albumins (HSA and BSA, respectively) and short peptide analogues was performed using potentiometry and spectroscopic techniques. It was found that while qualitative aspects of interaction (spectra and structures of complexes, order of reactions) could be reproduced, the quantitative parameters (stability and rate constants) could not.

9p research12 01-06-2013 38 4   Download

Human novel reductase 1 (NR1) is an NADPH dependent diflavin oxidoreductase related to cytochrome P450reduc-tase (CPR). The FAD/NADPH- and FMN-binding domains of NR1 have been expressed and purified and their redox properties studied by stopped-flow and steady-state kinetic methods, and by potentiometry.

12p tumor12 20-04-2013 41 2   Download

This study on human cytochrome P450 reductase (CPR) presents a com-prehensive analysis of the thermodynamic and kinetic effects of pH and solvent on two- and four-electron reduction in this diflavin enzyme. pH-dependent redox potentiometry revealed that the thermodynamic equilibrium between various two-electron reduced enzyme species (FMNH • ,FADH • ; FMN,FADH2; FMNH2 ,FAD) is independent of pH.

18p galaxyss3 07-03-2013 45 3   Download

Oxidation/Reduction Reactions Reactions in which electrons are transferred from one reactant to another. Example I-1 Cu2+ + Zn (s) Cu (s) + Zn2+ Cu2+ has undergone reduction and is reduced. Oxidizing reagent: Cu2+. Reducing agent: Zn. Zn has undergone oxidation and is oxidized. .

91p doilan 26-01-2013 66 10   Download