Precise genetic editing

Part 2 book "Medical genetics" includes contents: Disease gene identification, immunogenetics, cancer genetics, genetic basis of development, multifactorial inheritance and common diseases, genetic testing and gene therapy, genetics and precision medicine, clinical genetics and genetic counseling.

206p oursky01 17-07-2023 2 0   Download

Base editing installs a precise nucleotide change in specific gene loci without causing a double-strand break. Its efficiency in human embryos is generally low, limiting its utility in functional genetic studies.

7p vigalileogalilei 27-02-2022 11 1   Download

The CRISPR/Cas9 system has become an efficient gene editing method for generating cells carrying precise gene mutations, including the rearrangement and deletion of chromosomal segments. However, whether an entire chromosome could be eliminated by this technology is still unknown.

18p vialfrednobel 29-01-2022 14 0   Download

Large-scale genetic screening using CRISPR-Cas9 technology has emerged as a powerful approach to uncover and validate gene functions. The ability to control the timing of genetic perturbation during CRISPR screens will facilitate precise dissection of dynamic and complex biological processes.

15p visilicon2711 20-08-2021 4 1   Download

The recent advances in agricultural biotechnology and genetic engineering have brought numerous benefits to the food and agricultural sector by improving the essential characteristics of plant agronomic traits. Targeted genome editing using sequence specific nucleases (SSNs) provides a general method for inducing targeted deletions, insertions and precise sequence changes in a broad range of organisms and cell types.

15p chauchaungayxua11 23-03-2021 12 2   Download

Recently, the CRISPR/Cas9 system has been widely used to precisely edit plant genomes. Due to the difficulty in Agrobacterium-mediated genetic transformation of wheat, the reported applications in CRISPR/Cas9 system were all based on the biolistic transformation.

12p vishikamaru2711 29-04-2020 6 0   Download

CRISPR/Cas9 is widely used for precise genetic editing in various organisms. CRISPR/Cas9 editing may in many plants be hampered by the presence of complex and high ploidy genomes and inefficient or poorly controlled delivery of the CRISPR/Cas9 components to gamete cells or cells with regenerative potential.

12p vihamax2711 21-04-2020 11 0   Download

Reversal of carbapenem-resistance in Shewanella algae by CRISPR/Cas9 genome editing Antibiotic resistance in pathogens is a growing threat to human health. Of particular concern is resistance to carbapenem, which is an antimicrobial agent listed as critically important by the World Health Organization. With the global spread of carbapenem-resistant organisms, there is an urgent need for new treatment options. Shewanella algae is an emerging pathogen found in marine environments throughout the world that has increasing resistance to carbapenem.

9p caygaocaolon1 13-11-2019 17 0   Download

Ten years ago, as we put together the first edition of Men’s Health, we asked an important question: ‘Why do men die on average five years younger than women?’ A decade later, although we now have at least a partial answer, namely that men look after themselves less well than women, the so-called gender gap still persists. The key issue then for readers of the third edition of this book is what can be done to narrow this gap, thereby allowing men to live longer and healthier lives. This is precisely the problem that we have asked each of our authors to address.

585p crius75 07-01-2013 60 4   Download